In vivo detection of free radicals induced by diethylnitrosamine in rat liver tissue

Yamada, Ken Ichi; Yamamiya, Ikuo; Utsumi, Hideo

doi:10.1016/j.freeradbiomed.2006.01.031

Cited by 71 publications

(56 citation statements)

References 35 publications

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“…41 The significant reduction in the specific activity of ALP in the liver of rats with a corresponding increase in the serum is an indication of loss of integrity in the liver plasma membrane. The decrease in the specific activity of ALP in the liver of rats might have resulted from the peroxidation of the polyunsaturated fatty acids of the plasma membrane by ROS (O 2 À , OH, RO 2 , H 2 O 2 and ONOO À ) generated during NDEA metabolism. 3 The capability of polyphenolic extract of B. sapida arilli to prevent NDEA-mediated alteration in ALP could be attributed to the free radical and ROS scavenging capability of B. sapida arilli made possible by flavonoids, polyphenol present in B. sapida arilli.…”

Section: Discussionmentioning

confidence: 99%

“…The capability of the extract to reverse this trend in a manner similar to vitamin C suggests antioxidant potential of the extract. This amelioration may be adduced to the capability of the extract to scavenge ROS (O 2 À , OH, RO 2 , H 2 O 2 and ONOO À ) generated during NDEA metabolism. 3 Oxidative damage to cellular macromolecules (lipids, proteins, DNA etc.)…”

Section: Discussionmentioning

confidence: 99%

“…1 Microsomal metabolism of NDEA by cytochrome P 450 monooxidase system leads to the generation of free radicals, lipid-derived and hydroxyl radical 2 and reactive oxygen species. 3 These reactive species causes oxidative damage leading to cytotoxicity, carcinogenicity and mutagenicity.…”

Section: Introductionmentioning

confidence: 99%

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N-nitrosodiethylamine induced redox imbalance in rat liver: Protective role of polyphenolic extract of Blighia sapida arilli

Oloyede

Ajiboye

Komolafe

2013

Free Radicals and Antioxidants

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a b s t r a c tAim: This study investigates the reactive oxygen species detoxification potentials of polyphenolic extract of Blighia sapida arilli in the liver of N-nitrosodiethylamine-treated rats. Methods: Male rats, weighing 158 AE 2.9 g were completely randomized into 7 groups (AeG) of five rats each. Rats in C, D, E and F were administered orally once daily at 24 h interval for 7 days with 1000, 250, 500 and 1000 mg/kg body weight of polyphenolic extract of B. sapida arilli respectively. Group G was given 100 mg/kg body weight of vitamin C. On the sixth day, group B, D, E, F and G were administered with 100 mg/kg body weight N-nitrosodiethylamine (NDEA). Group A, which served as the control was treated like the test groups except, that the animals received distilled water only. Results: Reactive oxygen species detoxifying enzymes (superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase and glucose 6-phosphate dehydrogenase) activities were significantly (P < 0.05) induced by B. sapida arilli. These inductions significantly (P < 0.05) attenuated the decrease in reactive oxygen species detoxifying enzymes mediated by NDEA treatment and compared favourably with vitamin C. NDEA-mediated elevation in the concentrations of oxidative stress biomarkers; malondialdehyde, conjugated dienes, lipid hydroperoxides, protein carbonyl and percentage DNA fragmentation were significantly (P < 0.05) lowered by polyphenolic extract of B. sapida arilli. Conclusion: Overall, the results obtained from this study revealed that the polyphenolic extract from B. sapida arilli enhanced the detoxification of reactive oxygen species in NDEA-treated rats. The polyphenols also prevented the peroxidation of lipid, oxidation of proteins as well as fragmentation of DNA component in the liver of rats and hence gave the evidence of possible prophylactic potentials of B. sapida arilli.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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N-nitrosodiethylamine induced redox imbalance in rat liver: Protective role of polyphenolic extract of Blighia sapida arilli

Oloyede

Ajiboye

Komolafe

2013

Free Radicals and Antioxidants

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show abstract

“…Nitroxides have several potential advantages as spin probes (Kuppusamy et al, 2002;Yamada et al, 2006), spin labels (Borbat et al, 2001), contrast agents (Soule et al, 2007) and antioxidants (Wilcox and Pearlman, 2008). These applications are based on the complementary nature of the radical moieties in nitroxides; paramagnetism allows them to react with free radicals and interact with nuclear spin.…”

Section: Common Reactivity Of Nitroxidesmentioning

confidence: 99%

Synthesis and Utilization of α-Substituted Nitroxides

Yamasaki

Mito

Matsuoka³

et al. 2012

Nitroxides - Theory, Experiment and Applications

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“…Applications of EPR for in vivo measurements has been difficult because 1) the absolute concentration of biological radicals in a steady condition is quite low and 2) microwaves used in the conventional X-band EPR system causes dielectric loss by body fluid. The recent development of a nitroxyl radical-spin probe method and low-frequency L-band EPR has circumvented these problems and in vivo EPR measurement is coming into use (4)(5)(6)(7)(8)(9)(10)(11). In the EPR spin probe method, a paramagnetically stable spin probe, which is EPR-positive in stable conditions, is injected into the animals.…”

Section: Introductionmentioning

confidence: 99%