In vivo delivery of peptides and Toll-like receptor ligands by mannose-functionalized polymeric nanoparticles induces prophylactic and therapeutic anti-tumor immune responses in a melanoma model

Silva, Joana M.; Zupančič, Eva; Vandermeulen, Gaëlle; Oliveira, Vanessa G.; Salgado, Ana; Videira, Mafalda; Gaspar, Maria Manuela; Graça, Luís; Préat, Véronique; Florindo, Helena F.

doi:10.1016/j.jconrel.2014.11.033

Cited by 125 publications

(99 citation statements)

References 53 publications

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“…Previous studies have indicated that Toll-like receptor 4 (TLR4) plays a vital role in the pro-inflammatory interaction between cells and biomaterials [47]. TLR4 is involved in the uptake of particles, such as titanium dioxide nanoparticles (TiO 2 NPs), to promote inflammatory responses [48].…”

Section: Discussionmentioning

confidence: 99%

Exposure of the murine RAW 264.7 macrophage cell line to dicalcium silicate coating: assessment of cytotoxicity and pro-inflammatory effects

Chen

Liu

Wei

et al. 2016

J Mater Sci: Mater Med

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Inflammatory effects are significant elements of the immune response to biomaterials. Previously, we reported inflammatory effects in response to dicalcium silicate (Ca2SiO4, C2S) particles. However, the immunological effects of C2S coatings have not been studied. C2S often used as coatings materials in orthopedic and dentistry applications. It may have different effect from C2S particles. Further, it remains unclear whether C2S coating is equally biocompatible as 45S5 coating. The aim of this study was to test the cytotoxicity and pro-inflammatory effects of C2S coating on RAW 264.7 macrophages. C2S and 45S5 coatings were characterized using scanning electron microscopy (SEM), atomic force microscopy (AFM), energy dispersive analysis (EDS) and X-ray diffraction (XRD). inductively coupled plasma optical emission spectroscopy (ICP-OES) was used to detect ionic concentrations after soaking coated discs in medium. The cytotoxicity of C2S and 45S5 coatings against RAW 264.7 macrophages was measured using the LDH Cytotoxicity Assay Kit, Cell Counting Kit-8 (CCK-8) assays and flow cytometry for apoptosis assays. The gene and protein expression of TNF-α, IL-6 and IL-1β were detected using RT-q PCR and ELISA, respectively. The tested coating materials are not cytotoxic to macrophages. The C2S-coated surface stimulated macrophages to express pro-inflammatory mediators, such as TNF-α, IL-6 and IL-1β, and C2S coating caused less IL-6 but greater IL-1β production than the 45S5 coating. C2S coating have no cytotoxicity when directly cultured with macrophages. C2S and 45S5 coatings both have the potential to induce pro-inflammatory effects, and the biocompatibility of C2S is similar to that of 45S5.

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Section: Discussionmentioning

confidence: 99%

Exposure of the murine RAW 264.7 macrophage cell line to dicalcium silicate coating: assessment of cytotoxicity and pro-inflammatory effects

Chen

Liu

Wei

et al. 2016

J Mater Sci: Mater Med

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“…21 In a recent study, Silva et al investigated multiple factors that have an efficient impact on the therapeutic effect of a cancer vaccine. 84 These authors demonstrated that the multifunctional properties of NPs in terms of targeting, the synergy between TLR ligands and the relevance of combining multiple TAAs, including MHC class I-and class II-restricted peptides and co-entrapment of antigen/adjuvant have high cancer immunotherapeutic potential. For constructing this delivery system, two TLR ligands, Poly(I:C) and CpG, known to be Th1-immunopotentiators, were coentrapped along with melanoma-associated antigens in mannose- functionalized aliphatic polyester-based nanoparticles (NPs).…”

Section: Nanoparticle For Delivery Of Toll-like Receptor Ligandsmentioning

confidence: 99%

“…The nanoparticulate vaccines decreased the growth rate of murine B16F10 melanoma tumors in therapeutic and prophylatic settings. 84 In another study Roy et al used a TLR4 agonist as an immunostimlunat for enhancement the effect of chemotherapeutic agent. In this study paclitaxel as a cytotoxic drug was co-encapsulated with a TLR4 agonist through a PLGA based nanoparticle and evaluated its anticancer activity.…”

Section: Nanoparticle For Delivery Of Toll-like Receptor Ligandsmentioning

confidence: 99%

Multifunctional nanoparticles for cancer immunotherapy

Saleh

Shojaosadati

2016

Human Vaccines & Immunotherapeutics

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During the last decades significant progress has been made in the field of cancer immunotherapy. However, cancer vaccines have not been successful in clinical trials due to poor immunogenicity of antigen, limitations of safety associated with traditional systemic delivery as well as the complex regulation of the immune system in tumor microenvironment. In recent years, nanotechnology-based delivery systems have attracted great interest in the field of immunotherapy since they provide new opportunities to fight the cancer. In particular, for delivery of cancer vaccines, multifunctional nanoparticles present many advantages such as targeted delivery to immune cells, co-delivery of therapeutic agents, reduced adverse outcomes, blocked immune checkpoint molecules, and amplify immune activation via the use of stimuli-responsive or immunostimulatory materials. In this review article, we highlight recent progress and future promise of multifunctional nanoparticles that have been applied to enhance the efficiency of cancer vaccines.

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“…The spleens were removed aseptically, and splenocytes were isolated as previously described. 36 Then, 96-well plates were seeded with 1 × 10 6 cells in 100 μl of media per well. The cells were stimulated by adding 100 µl of 100 µg/ml of OVA solution (SigmaAldrich).…”

Section: Plasmidsmentioning

confidence: 99%

Coadministration of a Plasmid Encoding HIV-1 Gag Enhances the Efficacy of Cancer DNA Vaccines

et al. 2016

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In vivo delivery of peptides and Toll-like receptor ligands by mannose-functionalized polymeric nanoparticles induces prophylactic and therapeutic anti-tumor immune responses in a melanoma model

Cited by 125 publications

References 53 publications

Exposure of the murine RAW 264.7 macrophage cell line to dicalcium silicate coating: assessment of cytotoxicity and pro-inflammatory effects

Exposure of the murine RAW 264.7 macrophage cell line to dicalcium silicate coating: assessment of cytotoxicity and pro-inflammatory effects

Multifunctional nanoparticles for cancer immunotherapy

Coadministration of a Plasmid Encoding HIV-1 Gag Enhances the Efficacy of Cancer DNA Vaccines

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