In vivo degradation and neovascularization of silk fibroin implants monitored by multiple modes ultrasound for surgical applications

Li, Shouqiang; Yu, Dan; Ji, Huan; Zhao, Baocun; Ji, Lili; Leng, Xiaoping

doi:10.1186/s12938-018-0478-4

Cited by 17 publications

(23 citation statements)

References 34 publications

(31 reference statements)

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“…In the later stage, the echo of the sample was lower than that of the muscle. The echo of the SF scaffold during ultrasound in this study was different from the ndings by Li et al [22]. This is possible because SF can be processed into different constructs (e.g., porous scaffolds, lms, hydrogels, and nano/microspheres).…”

Section: Discussioncontrasting

confidence: 80%

“…It has been used in research that involves degrading biological materials; however, it is rarely used when researching SF scaffolds. Li et al [22] investigated the neovascularization and biodegradation of an SF gel in vivo using multiple mode ultrasound by quantifying the echo intensity, volume, and contrast enhancement of the SF gel implants. It showed that the silk gel implants appear as hypoechoic nodules in the early stages of the degradation, and there are clear boundaries under the skin.…”

Section: Discussionmentioning

confidence: 99%

“…In the later stage, the echo is lower than that of the muscle. Recent studies [22] have shown that the different forms of SF materials (e.g., gels) have different ultrasound performances. Therefore, future studies should focus on determining the ultrasonic performance for the different forms of SF materials.…”

Section: Discussionmentioning

confidence: 99%

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Application of an Ultrasound Semi-Quantitative Assessment in the Degradation of Silk Fibroin Scaffolds In Vivo

Li-hui

Gao

Sun

et al. 2021

Preprint

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Background: Research on the degradation of silk fibroin (SF) scaffolds in vivo lacks uniform and effective standards and experimental evaluation methods. This study aims to evaluate the application of ultrasound in assessing the degradation of SF scaffolds.Methods: Two groups of three-dimensional regenerated SF scaffolds (3D RSFs) were implanted subcutaneously into the backs of Sprague-Dawley rats. B-mode ultrasound and hematoxylin and eosin (HE) staining were performed on days 3, 7, 14, 28, 56, 84, 112, 140, and 196. The cross-sectional areas for two groups of 3D RSFs that were obtained using these methods were semi-quantitatively analyzed and compared to evaluate the biodegradation of the implanted RSFs.Results: Semi-quantitative analysis of the cross-sectional areas detected using B-mode ultrasound revealed that the degradations of the two 3D RSF groups were significantly different. The degradation rate of the SF-B group was found to be higher than that of the SF-A group. This was consistent with the semi-quantitative detection results for HE staining. Regression analysis showed that the results of the B-mode ultrasound and HE staining were correlated in both groups, indicating that B-mode ultrasound is a reliable method to evaluate the SF scaffold degradation in vivo. As the SF scaffold degraded, its echo gradually decreased. In the early stages of degradation, the echo of the SF scaffold was higher than that of the muscle. In the middle stage of degradation, the echo was equal to that of the muscle. In the later stage, the echo was lower than that of the muscle.Conclusions: This study suggests that B-mode ultrasound can clearly display the implanted SF scaffolds non-invasively and monitor the degradation of the different SF scaffolds after implantation in living organisms in real-time.

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Section: Discussioncontrasting

confidence: 80%

Section: Discussionmentioning

confidence: 99%

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Application of an Ultrasound Semi-Quantitative Assessment in the Degradation of Silk Fibroin Scaffolds In Vivo

Li-hui

Gao

Sun

et al. 2021

Preprint

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“…Silk fiber processing solvents can affect the secondary structure and biodegradability of silk. Organic solvent-based silk takes a year or more to get completely absorbed in vivo (Zhang et al, 2009 ), while aqueous solvent-based silk only requires a maximum of 6 months to get completely absorbed in vivo (Li S. et al, 2018 ). This property is helpful in repairing critical bone defects as silk scaffolds can provide sufficient structural support until native bone is completely regenerated (Mandal and Kundu, 2009 ).…”

Section: Natural Polymers: Organic Biomaterials Extracted From Organismentioning

confidence: 99%

Stem Cell-Friendly Scaffold Biomaterials: Applications for Bone Tissue Engineering and Regenerative Medicine

Zhang

Zhao

et al. 2020

Front. Bioeng. Biotechnol.

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Bone is a dynamic organ with high regenerative potential and provides essential biological functions in the body, such as providing body mobility and protection of internal organs, regulating hematopoietic cell homeostasis, and serving as important mineral reservoir. Bone defects, which can be caused by trauma, cancer and bone disorders, pose formidable public health burdens. Even though autologous bone grafts, allografts, or xenografts have been used clinically, repairing large bone defects remains as a significant clinical challenge. Bone tissue engineering (BTE) emerged as a promising solution to overcome the limitations of autografts and allografts. Ideal bone tissue engineering is to induce bone regeneration through the synergistic integration of biomaterial scaffolds, bone progenitor cells, and bone-forming factors. Successful stem cell-based BTE requires a combination of abundant mesenchymal progenitors with osteogenic potential, suitable biofactors to drive osteogenic differentiation, and cell-friendly scaffold biomaterials. Thus, the crux of BTE lies within the use of cell-friendly biomaterials as scaffolds to overcome extensive bone defects. In this review, we focus on the biocompatibility and cell-friendly features of commonly used scaffold materials, including inorganic compound-based ceramics, natural polymers, synthetic polymers, decellularized extracellular matrix, and in many cases, composite scaffolds using the above existing biomaterials. It is conceivable that combinations of bioactive materials, progenitor cells, growth factors, functionalization techniques, and biomimetic scaffold designs, along with 3D bioprinting technology, will unleash a new era of complex BTE scaffolds tailored to patient-specific applications.

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“…The development of silk fibroin properties is very important in order to meet various application requirements. Therefore, researchers have carried out a variety of studies aimed at extending the application of silk fibroin from hydrophobic to hydrophilic material [47,48], as well as from filaments to films, sheets, and scaffolds [46,49,50].…”

Section: Introductionmentioning

confidence: 99%

Degradation Behavior of Silk Fibroin Biomaterials - A Review

Purnomo¹,

Setyarini²,

Sulistyaningsih³

2019

JESTR

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Silk fibroin-based biomaterials have developed widely for biomedical applications including soft tissue engineering and bone implants. Controlling the degradation rate is very notable in maintaining the performance of biomaterials in carrying out their functions. This review was focused on the process of biodegradation of silk fibroin and its controllers including among other factors that influence the degradation process, mechanisms, behavior, the role of ultraviolet (UV) radiation, and the effect of molecular weight. All studies in this paper provide more in-depth knowledge about the biodegradation of silk fibroin enzymatically as well as UV radiation effect.

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In vivo degradation and neovascularization of silk fibroin implants monitored by multiple modes ultrasound for surgical applications

Cited by 17 publications

References 34 publications

Application of an Ultrasound Semi-Quantitative Assessment in the Degradation of Silk Fibroin Scaffolds In Vivo

Application of an Ultrasound Semi-Quantitative Assessment in the Degradation of Silk Fibroin Scaffolds In Vivo

Stem Cell-Friendly Scaffold Biomaterials: Applications for Bone Tissue Engineering and Regenerative Medicine

Degradation Behavior of Silk Fibroin Biomaterials - A Review

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