In vivo Comet assay – statistical analysis and power calculations of mice testicular cells

DNA damage in mouse organs and in human sperm cells by bisphenol A

“…The cryotubes with the testes were each prepared as described in Hansen et al (2014). Thirty µL of the different tissue mixtures was applied onto one sample area of two gels on two different slides (one gel on one slide) consisting of 20 gels (CometSlide™ HT, Trevigen).…”

“…The positive control dose group was compared to the control dose group with an unpaired two-tailed t-test. Prior to statistical analysis the data were log transformed (natural logarithm) as recommended for in vivo comet assay data by Hansen et al 2014 andSmith et al 2008. The human sperm cells comet assay data were analyzed by means of one-way ANOVA with Dunnett's test to compare the dosed groups with the control group. The positive control group (exposed to EMS) was analyzed against the control dose group by use of a two-tailed, unpaired t-test.…”

Assessing testicular germ cell DNA damage in the comet assay; introduction of a proof‐of‐concept

“…The OECD suggests that protocol modifications and validation studies are necessary before TG 489 can be revised to include an analysis of testicular germ cells. Nevertheless, the comet assay has been used to measure DNA damage in gonadal cells in both research settings (Asare et al, 2016; Bjorge et al, 1995; 1996a; Brunborg et al, 2015; Olsen et al, 2001; 2003; Collins, 2004; Olsen et al, 2005; Olive & Banath, 2006; Speit & Hartmann, 2006; Dhawan et al, 2009; Hansen et al, 2010; 2014; Graupner et al, 2014; 2015; Gutzkow et al, 2016 Graupner et al, 2017; Sharma et al, 2018) and in a regulatory perspective (Brendler‐Schwaab et al, 2005; Vasquez, 2012; Graupner et al, 2014; Frotschl, 2015; Koppen et al, 2017).…”

Comet Assay on Toxicogenetics; Several Studies in Recent Years on Several Genotoxicological Agents

“…The monitoring of the surrounding environment for chemicals and compounds with possible genotoxic activity is of high priority [2]. Thus, the development of instruments for identifying risky chemicals and the understanding of their toxicity mecanism is a major objective for scientific research [3]. A number of assays exist for detection of genotoxicity in a variety of experimental systems, some of them with limited use due to complicated technical setup, the single cell gel electrophoresis assay also defines the Comet assay [3], discovered for the first time in 1984 by two Swedish researchers, Ostling and Johanson [4].…”