In vivo characterization of neural crest-derived fibro/adipogenic progenitor cells as a likely cellular substrate for craniofacial fibrofatty infiltrating disorders

Paylor, Ben; Joe, Aaron W.; Rossi, Fábio; Lemos, Darío R.

doi:10.1016/j.bbrc.2014.07.089

Cited by 12 publications

(14 citation statements)

References 13 publications

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“…Moreover, NCFAP Brdu positivity increased from 0.81 ± 0.44% at baseline to 70.7 ± 12.2% 3 days after injury, while Brdu positivity in MFAPs only increased from 0.93 ± 0.92% to 48.3% ± 8.3 over the same time period, representing a significant difference in change of proliferation between the cell populations. Similar findings were observed in a study from Paylor et al Of note, in our study MA FAPs were more numerous than hindlimb FAPs, and SS FAPs exhibited significantly higher proliferation indices as demonstrated by Ki67 and Brdu staining. Due to the anatomic proximity of MA and RC muscles, it is possible they possess similar NCFAP:MFAP ratios, which may account for some of the cell behavior differences observed in our study.…”

Section: Discussionsupporting

confidence: 92%

Rotator Cuff Fibro‐Adipogenic Progenitors Demonstrate Highest Concentration, Proliferative Capacity, and Adipogenic Potential Across Muscle Groups

Lee

Agha

Liu

et al. 2019

Journal Orthopaedic Research

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Fatty infiltration (FI) of rotator cuff (RC) muscles is common in patients with RC tears. Studies have demonstrated that fibro-adipogenic progenitors (FAPs), a population of resident muscle stem cells, are the main contributors of FI, which adversely affects muscle quality and RC repair success. Although FI is common in RC injuries, it is not frequently reported after other musculotendinous injuries. Additionally, studies have shown the development of different pathology patterns across muscle groups suggestive of intrinsic differences in cellular composition and behavior. This study evaluates FAP distribution and differentiation properties across anatomic locations in mice. Muscles from seven different anatomic locations were harvested from PDGFRα-eGFP FAP reporter mice. FAPs were quantified using histology and FACS sorting with BD Aria II with CD31 − /CD45 − /Integrinα7 − /Sca-1 + and PDGFRα reporter signal (n = 3 per muscle). The cells were analyzed for adipogenesis using immunocytochemistry and for proliferation properties with Brdu-Ki67 staining. In a separate group of mice, RC and tibialis anterior muscles received glycerol injection and were harvested after 2 weeks for FI quantification (n = 4). One-way analysis of variance was used for statistical comparisons among groups, with significance at p < 0.05. FAPs from the RC, masseter, and paraspinal muscles were more numerous and demonstrated greater proliferative capacity and adipogenic potency than those from the tibialis anterior and gastrocnemius. The RC demonstrated significantly greater levels of FI than the tibialis anterior after glycerol-injection injury. Clinical Significance: This study suggests differences in FAP distribution and differentiation characteristics may account for the propensity to develop FI in RC tears as compared with other musculotendinous injuries.

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Section: Discussionsupporting

confidence: 92%

Rotator Cuff Fibro‐Adipogenic Progenitors Demonstrate Highest Concentration, Proliferative Capacity, and Adipogenic Potential Across Muscle Groups

Lee

Agha

Liu

et al. 2019

Journal Orthopaedic Research

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“…Magnetic cell sorting and fluorescent activated cell sorting (FACS) have been developed to sort subpopulations of stem cells on the basis of the cell surface marker profiles with some degree of success2021222324. It has been shown that PDGFRα, Sca1, CD34 or CD15 are typically used as specific cell surface markers for adipogenic cells2122, while CD56 is extensively used as a marker for identification and isolation of myogenic cells from human skeletal muscle, although CD56 + cell subpopulation was reported to exhibit both myogenic and adipogenic potential23.…”

Section: Discussionmentioning

confidence: 99%

A potential regulatory network underlying distinct fate commitment of myogenic and adipogenic cells in skeletal muscle

Sun

Qin

et al. 2017

Sci Rep

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Mechanism controlling myo-adipogenic balance in skeletal muscle is of great significance for human skeletal muscle dysfunction and myopathies as well as livestock meat quality. In the present study, two cell subpopulations with particular potency of adipogenic or myogenic differentiation were isolated from neonatal porcine longissimus dorsi using the preplate method to detect mechanisms underlying distinct fate commitment of myogenic and adipogenic cells in skeletal muscle. Both cells share a common surface expression profile of CD29+CD31−CD34−CD90+CD105+, verifying their mesenchymal origin. A total of 448 differentially expressed genes (DEGs) (FDR < 0.05 and |log2 FC| ≥ 1) between two distinct cells were identified via RNA-seq, including 358 up-regulated and 90 down-regulated genes in myogenic cells compared with adipogenic cells. The results of functional annotation and enrichment showed that 42 DEGs were implicated in cell differentiation, among them PDGFRα, ITGA3, ITGB6, MLCK and MLC acted as hubs between environment information processing and cellular process, indicating that the interaction of the two categories exerts an important role in distinct fate commitment of myogenic and adipogenic cells. Particularly, we are first to show that up-regulation of intracellular Ca2+-MLCK and Rho-DMPK, and subsequently elevated MLC, may contribute to the distinct commitment of myogenic and adipogenic lineages via mediating cytoskeleton dynamics.

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“…A large number of cell types identified within LSL specimens may be derived from or associated with neural crest cells. A number of recent publications have demonstrated the ability of neural crest cells to differentiate into an even larger range of cell types than thought previously, including adipocytes and bone marrow . This raises the possibility that neural crest differentiation may have a role in the embryogenesis of LSL and casts doubt on the previous notion of premature dysjunction.…”

Section: Discussionmentioning

confidence: 95%

“…recent publications have demonstrated the ability of neural crest cells to differentiate into an even larger range of cell types than thought previously, including adipocytes and bone marrow. [17][18][19][20] This raises the possibility that neural crest differentiation may have a role in the embryogenesis of LSL and casts doubt on the previous notion of premature dysjunction. 'Complex' LSLs (transitional and chaotic) are related intimately to the conus; indeed, the precise position of the conus may be difficult to identify on MRI in these types.…”

Section: Discussionmentioning

confidence: 99%

“…26 In addition, our understanding of the cell biology of neural crest cells is expanding rapidly, with neural crest stem cells being identified in a range of different tissue types. [17][18][19][20] These cells have the potential to differentiate and even transdifferentiate into the range of cell types seen within the majority of samples within our series.…”

Section: Cerebellar Tissue Ganglion Cells Ependymal Structuresmentioning

confidence: 95%

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The pathology of lumbosacral lipomas: macroscopic and microscopic disparity have implications for embryogenesis and mode of clinical deterioration

et al. 2018

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AimsLumbosacral lipomas (LSL) are congenital disorders of the terminal spinal cord region that have the potential to cause significant spinal cord dysfunction in children. They are of unknown embryogenesis with variable clinical presentation and natural history. It is unclear whether the spinal cord dysfunction reflects a primary developmental dysplasia or whether it occurs secondarily to mechanical traction (spinal cord tethering) with growth. While different anatomical subtypes are recognised and classified according to radiological criteria, these subtypes correlate poorly with clinical prognosis. We have undertaken an analysis of surgical specimens in order to describe the spectrum of histological changes that occur and have correlated the histology with the anatomical type of LSL to determine if there are distinct histological subtypes.Methods and resultsThe histopathology was reviewed of 64 patients who had undergone surgical resection of LSL. The presence of additional tissues and cell types were recorded. LSLs were classified from pre‐operative magnetic resonance imaging (MRI) scans according to Chapman classification. Ninety‐five per cent of the specimens consisted predominantly of mature adipocytes with all containing thickened bands of connective tissue and peripheral nerve fibres, 91% of samples contained ectatic blood vessels with thickened walls, while 22% contained central nervous system (CNS) glial tissue. Additional tissue was identified of both mesodermal and neuroectodermal origin.ConclusionsOur analysis highlights the heterogeneity of tissue types within all samples, not reflected in the nomenclature. The diversity of tissue types, consistent across all subtypes, challenges currently held notions regarding the embryogenesis of LSLs and the assumption that clinical deterioration is due simply to tethering.

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In vivo characterization of neural crest-derived fibro/adipogenic progenitor cells as a likely cellular substrate for craniofacial fibrofatty infiltrating disorders

Cited by 12 publications

References 13 publications

Rotator Cuff Fibro‐Adipogenic Progenitors Demonstrate Highest Concentration, Proliferative Capacity, and Adipogenic Potential Across Muscle Groups

Rotator Cuff Fibro‐Adipogenic Progenitors Demonstrate Highest Concentration, Proliferative Capacity, and Adipogenic Potential Across Muscle Groups

A potential regulatory network underlying distinct fate commitment of myogenic and adipogenic cells in skeletal muscle

The pathology of lumbosacral lipomas: macroscopic and microscopic disparity have implications for embryogenesis and mode of clinical deterioration

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