In Vivo Characterization of Hyalonect, a Novel Biodegradable Surgical Mesh

Rhodes, Nicholas P.; Hunt, John A.; Longinotti, Cristina; Pavesio, Alessandra

doi:10.1016/j.jss.2010.09.015

Cited by 17 publications

(19 citation statements)

References 41 publications

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“…There were 21 studies using a small animal model (Table 4) [55,60,62,76,82,111-126], and only six studies using a large animal model (Table 5) [127-132]. As in maxillofacial animal studies, superior bone healing has also been observed in long bones treated with a barrier-membrane compared to the non-treated defects using bioabsorbable as well as non-resorbable membranes [111,117,118,121].…”

Section: Introductionmentioning

confidence: 99%

The role of barrier membranes for guided bone regeneration and restoration of large bone defects: current experimental and clinical evidence

Dimitriou

Mataliotakis

Calori

et al. 2012

BMC Med

302

280

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Treatment of large bone defects represents a great challenge in orthopedic and craniomaxillofacial surgery. Although there are several methods for bone reconstruction, they all have specific indications and limitations. The concept of using barrier membranes for restoration of bone defects has been developed in an effort to simplify their treatment by offering a sinlge-staged procedure. Research on this field of bone regeneration is ongoing, with evidence being mainly attained from preclinical studies. The purpose of this review is to summarize the current experimental and clinical evidence on the use of barrier membranes for restoration of bone defects in maxillofacial and orthopedic surgery. Although there are a few promising preliminary human studies, before clinical applications can be recommended, future research should aim to establish the 'ideal' barrier membrane and delineate the need for additional bone grafting materials aiming to 'mimic' or even accelerate the normal process of bone formation. Reproducible results and long-term observations with barrier membranes in animal studies, and particularly in large animal models, are required as well as well-designed clinical studies to evaluate their safety, efficacy and cost-effectiveness.

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Section: Introductionmentioning

confidence: 99%

The role of barrier membranes for guided bone regeneration and restoration of large bone defects: current experimental and clinical evidence

Dimitriou

Mataliotakis

Calori

et al. 2012

BMC Med

302

280

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“…One such biosynthetic material is Hyalonect ® , which has been used previously for chondral lesions and also has the potential to participate in periosteal regeneration due to its hyaluronate structure, which plays a role in extracellular matrix formation, angiogenesis, and osteogenesis [ 7 , 10 , 11 ]. Rhodes et al [ 5 ] reported that Hyalonect ® is capable of restoring the function of damaged connective tissues such as the periosteum without interfering with the natural tissue repair process.…”

Section: Discussionmentioning

confidence: 99%

“…Several studies have demonstrated osteoblastic activity-promoting effects of a hyaluronan-based mesh. In one such preclinical study, conducted by Rhodes et al, holes were drilled in the humerus of the canines [ 5 ]. The holes were then filled with bone graft materials and surrounded with a hyaluronan-based mesh.…”

Section: Discussionmentioning

confidence: 99%

“…Under such conditions, a material can play a role to cover fractured or defective bone. Innovations and technological advances have rendered this possible through the use of a hyaluronan-based mesh, which can be used to cover fractured or grafted bone and can also provide a scaffold to keep osteoprogenitor cells in place [ 5 – 7 ]. Hyalonect ® , a bio-resorbable knitted mesh used during orthopedic trauma and reconstructive surgery, is composed of HYAFF ® , a benzyl ester of hyaluronic acid (HA) and natural component of the extracellular matrix.…”

Section: Introductionmentioning

confidence: 99%

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Scintigraphic evaluation of the osteoblastic activity of rabbit tibial defects after HYAFF11 membrane application

et al. 2016

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BackgroundAn unfavorable condition for bone healing is the presence of bone defects. Under such conditions, a material can play a role to cover fractured or defective bone. Technological advances now allow for the use of such material. Hyalonect® (Fidia Advanced Biopolymers SLR, Italy), a novel membrane comprising knitted fibers of esterified hyaluronan (HYAFF11) can be used to cover fractured or grafted bone and can also serve as a scaffold to keep osteoprogenitor cells in place. The aim of this study was to compare osteoblastic activity by the use of scintigraphic methods in defective rabbit tibias during early-phase bone healing with or without a hyaluronan-based mesh.MethodsTwo groups (A and B) of New Zealand albino rabbits were used; each group included 10 animals. Operations on all rabbits were performed under general anesthesia. We also resected 10-mm bone segments from each animal’s tibial diaphysis. After resection, tibias with defects were fixed using Kirschner wires. In group A, no hyaluronan-based mesh was used. In group B, tibial segmental defects were enclosed with a hyaluronan-based mesh. The rabbits were followed up for 4 weeks postoperatively, after which bone scintigraphic studies were performed on each animal to detect and compare osteoblastic activity.ResultsThe mean count in the fracture side of the hyaluronan-based mesh group was significantly higher compared to that of the group A (p = 0.019). However, there was no significant difference between group B and control rabbits with respect to the mean count on the intact bone side (p = 0.437). The bone defect (fracture)/intact bone mean count ratio was significantly higher in group B compared to group A (p = 0.008).ConclusionsA hyaluronan-based mesh plays a role in promoting osteoblastic activity. Hyalonect® is suitable for restoring tissue continuity whenever the periosteal membrane is structurally impaired or inadequate. Our results demonstrated that, during early-phase bone healing, osteoblastic activity was increased in bone defect sites when a hyaluronan-based mesh was also used. The most important aspect of this study concerns its scintigraphy-based design. This study is the first to use a scintigraphic method to demonstrate the effectiveness of hyaluronic acid-based material for bone healing.

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“…Hyaluronic acid (HA)‐based materials have been used for the regeneration of skin (Tonello et al, 2005), cartilage tissue (Aigner et al, 1998), and bone (Mathews et al, 2012). Hyalonect, a marketed membrane comprised of knitted fibers of esterified hyaluronan, was found able to restore the function of damaged connective tissues, such as periosteum (Rhodes et al, 2011), and exhibited suitable features as a scaffold for myocardial tissue engineering (Gallina et al, 2012). Finally, recent studies proved HA‐based scaffolds as attractive substrates for skeletal muscle tissue engineering (Wang et al, 2009; Rossi et al, 2011; Monge et al, 2012).…”

mentioning

confidence: 99%

Human Ng2⁺ adipose stem cells loaded in vivo on a new crosslinked hyaluronic acid‐lys scaffold fabricate a skeletal muscle tissue

Desiderio

Francesco

Schiraldi³

et al. 2013

Journal Cellular Physiology

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Mesenchymal stem cell (MSC) therapy holds promise for treating diseases and tissue repair. Regeneration of skeletal muscle tissue that is lost during pathological muscle degeneration or after injuries is sustained by the production of new myofibers. Human Adipose stem cells (ASCs) have been reported to regenerate muscle fibers and reconstitute the pericytic cell pool after myogenic differentiation in vitro. Our aim was to evaluate the differentiation potential of constructs made from a new cross-linked hyaluronic acid (XHA) scaffold on which different sorted subpopulations of ASCs were loaded. Thirty days after engraftment in mice, we found that NG2(+) ASCs underwent a complete myogenic differentiation, fabricating a human skeletal muscle tissue, while NG2(-) ASCs merely formed a human adipose tissue. Myogenic differentiation was confirmed by the expression of MyoD, MF20, laminin, and lamin A/C by immunofluorescence and/or RT-PCR. In contrast, adipose differentiation was confirmed by the expression of adiponectin, Glut-4, and PPAR-γ. Both tissues formed expressed Class I HLA, confirming their human origin and excluding any contamination by murine cells. In conclusion, our study provides novel evidence that NG2(+) ASCs loaded on XHA scaffolds are able to fabricate a human skeletal muscle tissue in vivo without the need of a myogenic pre-differentiation step in vitro. We emphasize the translational significance of our findings for human skeletal muscle regeneration.

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In Vivo Characterization of Hyalonect, a Novel Biodegradable Surgical Mesh

Cited by 17 publications

References 41 publications

The role of barrier membranes for guided bone regeneration and restoration of large bone defects: current experimental and clinical evidence

The role of barrier membranes for guided bone regeneration and restoration of large bone defects: current experimental and clinical evidence

Scintigraphic evaluation of the osteoblastic activity of rabbit tibial defects after HYAFF11 membrane application

Human Ng2⁺ adipose stem cells loaded in vivo on a new crosslinked hyaluronic acid‐lys scaffold fabricate a skeletal muscle tissue

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In Vivo Characterization of Hyalonect, a Novel Biodegradable Surgical Mesh

Cited by 17 publications

References 41 publications

The role of barrier membranes for guided bone regeneration and restoration of large bone defects: current experimental and clinical evidence

The role of barrier membranes for guided bone regeneration and restoration of large bone defects: current experimental and clinical evidence

Scintigraphic evaluation of the osteoblastic activity of rabbit tibial defects after HYAFF11 membrane application

Human Ng2+ adipose stem cells loaded in vivo on a new crosslinked hyaluronic acid‐lys scaffold fabricate a skeletal muscle tissue

Contact Info

Product

Resources

About

Human Ng2⁺ adipose stem cells loaded in vivo on a new crosslinked hyaluronic acid‐lys scaffold fabricate a skeletal muscle tissue