In vivo characterization by means of digital cell image analysis of early-induced fractionated radiotherapy effects on the MXT mouse mammary tumor

El-Khattabi, Ouarda; Pauwels, Olivier S. G.; Simon, Stéphane; Gasperin, Plínio; Fruhling, Janos; Kiss, Róbert; Houtte, Paul Van

doi:10.1016/s0360-3016(96)00571-8

Cited by 3 publications

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“…This study has been the first study to demonstrate the potential of chromatin texture as a pretreatment biomarker of sensitivity to radiation. Although a number of previous studies have investigated posttreatment alterations in chromatin texture caused by radiation (25–27), none of these studies have quantitatively examined the pretreatment chromatin characteristics and their ability to predict response.…”

Section: Discussionmentioning

confidence: 99%

“…Cell cycle delay to allow for DNA repair may also alter the chromatin phenotype because of an accumulation of cells in the G2M phase. El‐Khattabi et al (25) in a study on chromatin modifications following the administration of fractionated irradiation to the MXT mouse mammary adenocarcinoma showed two phases of modification constituted by a decrease in chromatin condensation, followed by an increase. The authors suggest that modifications in the chromatin pattern level could be explained through DNA repair processes.…”

Section: Discussionmentioning

confidence: 99%

“…The value of chromatin phenotype in bladder cancer prognosis has also been confirmed by others (20, 21). Furthermore, nuclear texture analysis has also shown potential in assessing the relative sensitivity of bladder tumor cells to chemotherapeutic drugs (22, 23) and response to radiation (24–27). Taken together, these studies suggest that chromatin phenotype in bladder neoplasia, as measured using digital texture analysis, is a very strong biomarker.…”

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Prediction of radiosensitivity in human bladder cell lines using nuclear chromatin phenotype

et al. 2006

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Background: Nuclear texture analysis measures phenotypic changes in chromatin distribution within a cell nucleus, while the alkaline Comet assay is a sensitive method for measuring the extent of DNA breakage in individual cells. The authors aim to use both methods to provide information about the sensitivity of cells to ionizing radiation. Methods: The alkaline Comet assay was performed on six human bladder carcinoma cell lines and one human urothelial cell line exposed to γ‐radiation doses from 0 to 10 Gy. Nuclear chromatin texture analysis of 40 features was then performed in the same cell lines exposed to 0, 2, and 6 Gy to explore if nuclear phenotype was related to radiation sensitivity. Results: Comet assay results demonstrated that the cell lines exhibited different levels of radiosensitivity and could be divided into a radiosensitive and a radioresistant group at >6 Gy. Using stepwise discriminant analysis, a subset of important nuclear texture features that best discriminated between sensitive and resistant cell lines were identified A classification function, defined using these features, correctly classified 81.75% of all cells into their radiosensitive or radioresistant groups based on their pretreatment chromatin phenotype. Posttreatment chromatin changes also varied between cell lines, with sensitive cell lines showing a relaxed chromatin conformation following radiation, whereas resistant cell lines exhibited chromatin condensation. Conclusions: The authors conclude that the alkaline Comet assay and nuclear texture methodologies may prove to be valuable aids in predicting the response of tumor cells to radiotherapy. © 2006 International Society for Analytical Cytology

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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