In vivo characterisation of the human UCP3 gene minimal promoter in mice tibialis anterior muscles

Riquet, Franck B.; Rodriguez, Marianne; Guigal, Nolwen; Dromaint, Sandra; Naime, Isabelle; Boutin, Jean A.; Galizzi, Jean‐Pierre

doi:10.1016/j.bbrc.2003.10.034

Cited by 9 publications

(9 citation statements)

References 35 publications

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“…The 21 bp long MyoD-binding triple E-box only differs in three nucleotides of the first repeat and is otherwise identical. In contrast, two TATA-like boxes identified in the human promoter [26] are absent in the known rodent sequences. By in silico analysis of the hamster Ucp3 promoter we detected several putative nuclear receptor binding sites, among them 28 elements predicted to possibly bind Coup-TFII.…”

Section: Resultsmentioning

confidence: 99%

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et al. 2007

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Background: Ucp3 is an integral protein of the inner mitochondrial membrane with a role in lipid metabolism preventing deleterious effects of fatty acids in states of high lipid oxidation. Ucp3 is expressed in brown adipose tissue and skeletal muscle and controlled by a transcription factor complex including PPARalpha, MyoD and the histone acetyltransferase p300. Several studies have demonstrated interaction of these factors with chicken ovalbumin upstream promoter transcription factor II (Coup-TFII). This nuclear receptor is involved in organogenesis and other developmental processes including skeletal muscle development, but also co-regulates a number of metabolic genes. In this study we in silico analyzed the upstream region of Ucp3 of the Djungarian hamster Phodopus sungorus and identified several putative response elements for Coup-TFII. We therefore investigated whether Coup-TFII is a further player in the transcriptional control of the Ucp3 gene in rodents.

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Section: Resultsmentioning

confidence: 99%

“…The sequence alignment furthermore demonstrates that two TATA-like boxes present in the human promoter [26] are absent in rodent sequences including the hamster. This might prove to be crucial considering, that in the study of Riquet and coworkers (2003) the activities of human constructs were investigated in murine tissue.…”

Section: Discussionmentioning

confidence: 99%

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et al. 2007

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“…(19,26,65). Direct evidence of an interaction between PPAR␦ and UCP-3 is due to the finding that the UCP-3 gene promoter contains a PPAR response element (56). Specific PPAR␦ agonist treatment resulted in upregulation of UCP-3 that was mediated by fatty acids (65).…”

Section: Discussionmentioning

confidence: 97%

“…Gastrocnemius muscle was not investigated because Ucp-3 was not found to be differentially expressed in this tissue. Because UCP-3 is thought to be regulated by PPAR␦ (56) and is found primarily in muscle (11,23), we assessed expression of both proteins in soleus muscle after 24-h unloading. A representative Western blot of UCP-3 and PPAR␦ expression in soleus muscle of three ambulatory and five HLS animals is shown in Fig.…”

Section: Expression Of Ppars and Ppar-interacting And Ppar-regulated mentioning

confidence: 99%

Muscle unloading-induced metabolic remodeling is associated with acute alterations in PPARδ and UCP-3 expression

Mazzatti

Smith

Oita

et al. 2008

Physiological Genomics

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A number of physiological changes follow prolonged skeletal muscle unloading as occurs in spaceflight, bed rest, and hindlimb suspension (HLS) and also in aging. These include muscle atrophy, fiber type switching, and loss of the ability to switch between lipid and glucose usage, or metabolic inflexibility. The signaling and genomic events that precede these physiological manifestations have not been investigated in detail, particularly in regard to loss of metabolic flexibility. Here we used gene arrays to determine the effects of 24-h HLS on metabolic remodeling in mouse muscle. Acute unloading resulted in differential expression of a number of transcripts in soleus and gastrocnemius muscle, including many involved in lipid and glucose metabolism. These include the peroxisome proliferator-activated receptors (PPARs). In contrast to Ppar-alpha and Ppar-gamma, which were downregulated by acute HLS, Ppar-delta was upregulated concomitant with increased expression of its downstream target, uncoupling protein-3 (Ucp-3). However, differential expression of Ppar-delta was both acute and transient in nature, suggesting that regulation of PPARdelta may represent an adaptive, compensatory response aimed at regulating fuel utilization and maintaining metabolic flexibility.

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“…At this time, only a few target genes have been identified as having PPAR consensus elements within their specific promoters, although a number of metabolic genes are known to change with overexpression of PPARβ/δ, at this time, it is unclear if that is due to direct activation by PPARβ/δ or secondary effects of the overexpression. Specifically, two genes that are expressed in muscle, UCP3 and PDK4, are both known have PPAR elements in their promoter, with PDK4 specifically responding to activation of PPAR delta, while UCP3 appears to have the ability to respond to any of the three forms of PPARs [18–20]. Our data indicate that PDK4 expression was higher in the soleus muscle than the plantaris (data not shown), which corresponds with the increased PPARβ/δ expression in the soleus muscle.…”

Section: Discussionmentioning

confidence: 99%

Alterations in peroxisome proliferator-activated receptor mRNA expression in skeletal muscle after acute and repeated bouts of exercise

et al. 2009

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Peroxisome proliferator-activated receptors (PPAR) exist in three different forms, alpha (α), beta/ delta (β/δ), or gamma (γ), all of which are expressed in skeletal muscle and play a critical role in the regulation of oxidative metabolism. The purpose of this investigation was to determine the mRNA expression pattern of the different PPARs and peroxisome proliferator-activated receptor alpha coactivator-1 alpha (PGC-1α) in muscles that largely rely on either glycolytic (plantaris) or oxidative (soleus) metabolism. Further, we also examined the alterations in the PPARs mRNA expression after one bout of endurance exercise or after 12 weeks of exercise training in the different muscles. Female Sprague-Dawley rats (5-8 months) were either run on the treadmill once or exercised trained for 12 weeks. The muscles were removed 24 h after the last bout of exercise. The results demonstrated with the exception of PPAR β/δ, the PPAR mRNAs are expressed to a greater extent in the soleus muscle than in the plantaris muscle in sedentary animals. PPARγ was the least abundantly expressed PPAR in either the soleus or the plantaris muscle. With respect to exercise training, only PPARγ mRNA expression increased in the soleus muscle, while PPARβ/δ and γ mRNA levels increased in the plantaris muscle. Minimal changes were detected in any of the PPARs with one bout of exercise training. These results suggest that PPARγ mRNA levels are the lowest in skeletal muscle among all of the PPARs and PPARγ mRNA is the most responsive to changes in physical activity levels.

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In vivo characterisation of the human UCP3 gene minimal promoter in mice tibialis anterior muscles

Cited by 9 publications

References 35 publications

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Muscle unloading-induced metabolic remodeling is associated with acute alterations in PPARδ and UCP-3 expression

Alterations in peroxisome proliferator-activated receptor mRNA expression in skeletal muscle after acute and repeated bouts of exercise

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