In vivo characterisation of a Nafion®-modified Pt electrode for real-time nitric oxide monitoring in brain extracellular fluid

Finnerty, Niall J.; O'Riordan, Saidhbhe L.; Brown, Finbar O.; Serra, Pier Andrea; O’Neill, Robert; Lowry, John

doi:10.1039/c2ay05924j

Cited by 24 publications

(54 citation statements)

References 70 publications

(94 reference statements)

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“…injection it was important to examine the effect of normal saline administrations (0.9%) on the oxidation current. We have previously reported a significant but short lived change (22 ± 3 pA, p < 0.001, n = 9) from baseline levels in NO sensor's implanted in the striatum of Wistar rats following saline injection (Finnerty et al, 2012). Similar initial and brief injection effects have also been observed for tissue O 2 (Bolger et al, 2011) and regional cerebral blood flow (rCBF) (Lowry and Fillenz, 2001) during the injection of saline, with a comparable return to baseline levels.…”

Section: Systemic Administrationssupporting

confidence: 58%

“…The other end of the wire acted as the active (disk) surface. The electrode was modified as previously described (Brown et al, 2009;Brown and Lowry, 2003;Finnerty et al, 2012).…”

Section: No Sensor Preparationmentioning

confidence: 99%

“…Constant potential amperometry was performed using previously described methods (Brown et al, 2009;Finnerty et al, 2012). All data presented had baselines normalised to zero to show the change in current ( I) and reported concentration changes are based on a previously reported protocol (Finnerty et al, 2012).…”

Section: Instrumentation and Softwarementioning

confidence: 99%

“…All data presented had baselines normalised to zero to show the change in current ( I) and reported concentration changes are based on a previously reported protocol (Finnerty et al, 2012). The significance of differences observed was estimated using the Student's t-test for paired or unpaired observations where appropriate.…”

Section: Instrumentation and Softwarementioning

confidence: 99%

“…This has also been highlighted recently by Garthwaite, who expressed a great need for a reliable method of directly measuring endogenously generated NO in tissues with the necessary sensitivity or spatial and temporal precision (Garthwaite, 2008). We have previously reported the in vitro (Brown et al, 2009;Brown and Lowry, 2003) and in vivo (Finnerty et al, 2012) characterisation of a Nafion ® -modified Pt sensor designed for real-time monitoring of brain extracellular NO. In vitro findings confirmed that the Nafion ® (5/2) sensor had a response time suitable for in vivo monitoring, linearity over the relevant concentration range for NO, freedom from protein and lipid fouling, and minimal interference from a variety of endogenous species, including ascorbic acid, dopamine and serotonin over physiologically relevant concentration ranges.…”

Section: Introductionmentioning

confidence: 95%

See 4 more Smart Citations

Brain nitric oxide: Regional characterisation of a real-time microelectrochemical sensor

Finnerty¹,

O'Riordan²,

Pålsson³

et al. 2012

Journal of Neuroscience Methods

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This article appeared in a journal published by Elsevier. The attached copy is furnished to the author for internal non-commercial research and education use, including for instruction at the authors institution and sharing with colleagues.Other uses, including reproduction and distribution, or selling or licensing copies, or posting to personal, institutional or third party websites are prohibited. a b s t r a c tA reliable method of directly measuring endogenously generated nitric oxide (NO) in real-time and in various brain regions is presented. An extensive characterisation of a previously described amperometric sensor has been carried out in the prefrontal cortex and nucleus accumbens of freely moving rats. Systemic administration of saline caused a transient increase in signal from baseline levels in both the prefrontal cortex (13 ± 3 pA, n = 17) and nucleus accumbens (12 ± 3 pA, n = 8). NO levels in the prefrontal cortex were significantly increased by 43 ± 9 pA (n = 9) following administration of l-arginine. A similar trend was observed in the nucleus accumbens, where an increase of 44 ± 9 pA (n = 8) was observed when compared against baseline levels. Systemic injections of the non-selective NOS inhibitor l-NAME produced a significant decrease in current recorded in the prefrontal cortex (24 ± 6 pA, n = 5) and nucleus accumbens (17 ± 3 pA, n = 6). Finally it was necessary to validate the sensors functionality in vivo by investigating the effect of the interferent ascorbate on the oxidation current. The current showed no variation in both regions over the selected time interval of 60 min, indicating no deterioration of the polymer membrane. A detailed comparison identified significantly greater affects of administrations on NO sensors implanted in the striatum than those inserted in the prefrontal cortex and the nucleus accumbens.

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Section: Systemic Administrationssupporting

confidence: 58%

“…The other end of the wire acted as the active (disk) surface. The electrode was modified as previously described (Brown et al, 2009;Brown and Lowry, 2003;Finnerty et al, 2012).…”

Section: No Sensor Preparationmentioning

confidence: 99%

Section: Instrumentation and Softwarementioning

confidence: 99%

Section: Instrumentation and Softwarementioning

confidence: 99%

Section: Introductionmentioning

confidence: 95%

See 3 more Smart Citations

Brain nitric oxide: Regional characterisation of a real-time microelectrochemical sensor

Finnerty¹,

O'Riordan²,

Pålsson³

et al. 2012

Journal of Neuroscience Methods

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Electrochemical Sensors for Nitric Oxide Detection in Biological Applications

Scafa

et al. 2014

Electroanalysis

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Nitric oxide (NO) plays an important role in physiological processes and it has been confirmed some human diseases are related to its biological function. Electrochemical sensors provide an efficient way to explore the NO function in biological processes. This review details different kinds of electrochemical sensors used for NO concentration detection between 2008 and 2013 together with their application in biological samples. Four commonly used electrodes and different assisted analysis membranes used for contributions towards the development of the novel sensors were summarized. Electrochemical sensors employed to measure NO concentration in a single cell, cell culture, tissue homogenate, organ, in vivo, human being, as well as in plant were also detailed. The trends of developing novel NO sensors were outlooked in the last part.

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In vitro development and in vivo application of a platinum-based electrochemical device for continuous measurements of peripheral tissue oxygen

Finnerty

Bolger

2018

Bioelectrochemistry

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Acute limb ischaemia is caused by compromised tissue perfusion and requires immediate attention to reduce the occurrence of secondary complications that could lead to amputation or death. To address this, we have developed a novel platinum (Pt)-based electrochemical oxygen (O) device for future applications in clinical monitoring of peripheral tissue ischaemia. The effect of integrating a Pt pseudo-reference electrode into the O device was investigated in vitro with an optimum reduction potential of -0.80V. A non-significant (p=0.11) decrease in sensitivity was recorded when compared against an established Pt-based O sensor operating at -0.65V. Furthermore, a biocompatible clinical sensor (ClinOX) was designed, demonstrating excellent linearity (R=0.99) and sensitivity (1.41±0.02nAμM) for O detection. Significant rapid decreases in the O current during in vivo ischaemic insults in rodent limbs were reported for Pt-Pt (p<0.001) and ClinOX (p<0.01) and for ClinOX (p<0.001) in porcine limbs. Ex vivo sensocompatibility investigations identified no significant difference (p=0.08) in sensitivity values over 14days of exposure to tissue homogenate. The Pt-Pt based O design demonstrated high sensitivity for tissue ischaemia detection and thus warrants future clinical investigation.

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In vivo characterisation of a Nafion®-modified Pt electrode for real-time nitric oxide monitoring in brain extracellular fluid

Cited by 24 publications

References 70 publications

Brain nitric oxide: Regional characterisation of a real-time microelectrochemical sensor

Brain nitric oxide: Regional characterisation of a real-time microelectrochemical sensor

Electrochemical Sensors for Nitric Oxide Detection in Biological Applications

In vitro development and in vivo application of a platinum-based electrochemical device for continuous measurements of peripheral tissue oxygen

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