In vivo cellular and plasma velocities in microvessels of the cat mesentery

Starr, Matthew C.; Frasher, Wallace G.

doi:10.1016/0026-2862(75)90024-2

Cited by 17 publications

(4 citation statements)

References 5 publications

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“…These results are similar to those obtained in previous studies between control rats and rats chronically treated with tobacco smoke [8]. Since RBC velocity and volume flow of plasma are significantly correlated in capillary vessels [10], these findings imply that whole blood flow at the capillary level is compromised by chronic tobacco smoke or nicotine administration.…”

Section: Discussionsupporting

confidence: 82%

Effects of Chronic Nicotine Administration on RBC Velocity in Mesenteric Capillaries of the Rat

Richardson¹,

Morton²,

Howard³

1977

J Vasc Res

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The purpose of this study was to determine the effects of chronic administration of nicotine on red blood cell (RBC) velocity in single capillaries of the mesentery. Ten male rats consumed an average of 2.38 mg nicotine/kg/day, an approximate ‘2 pack’ equivalent, in their drinking water for a period of 15–20 weeks. At the end of this time RBC velocity was measured in single capillaries of the mesentery. The data were compared to RBC velocities measured in a group of matched control rats. Red cell velocities in the control and nicotine groups averaged 1.22 ± 0.23 and 0.59 ± 0.10 mm/sec SEM, respectively. These values were significantly different (p < 0.01). It is concluded that chronic administration of nicotine to rats in levels equivalent to tobacco smoking reduces resting capillary blood flow in the mesenteric circulation as indicated by differences in erythrocyte velocity.

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Section: Discussionsupporting

confidence: 82%

Effects of Chronic Nicotine Administration on RBC Velocity in Mesenteric Capillaries of the Rat

Richardson¹,

Morton²,

Howard³

1977

J Vasc Res

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“…Additionally, ͑1͒ the shapes of the velocity profiles in venules and arterioles differ from the parabolic 5 ͑i.e., are asymmetric͒; ͑2͒ the resistance to blood flow in living microvessels is higher than that in uniform tubes of the same diameters; 6 and ͑3͒ the distribution of cells in flow depends on the flow velocity and the cell concentration. 4,7,8 Nevertheless, as we show in the following, in selected blood vessels with a relatively fast flow velocity, flow is close to laminar, with most red blood cells ͑RBCs͒ concentrated near vessel axes with a mean centerline velocity 1.6 times higher than that of the mean blood flow 9 ͑i.e., natural hydrodynamic focusing occurs in vivo as it does in in vitro FC͒.…”

Section: Introductionmentioning

confidence: 64%

Integrated photothermal flow cytometry in vivo

2005

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The capability of integrated flow cytometry to detect, in real time, moving cells in their natural states in vivo is demonstrated in a study of circulating red and white blood cells in lymph and blood flow of rat mesentery. This system combines dual pump-probe photothermal (PT) techniques, such as PT imaging, the PT thermolens method, and PT velocimetry, with high-resolution (up to 0.3 microm), high-speed (up to 1000 fps) transmission digital microscopy (TDM) and fluorescence imaging. All PT techniques are based on irradiation of cells in rat mesenteric microvessels with a spectrally tunable laser pulse (420 to 570 nm, 8 ns, 0.1 to 300 microJ) and on detection of temperature-dependent variations of the refractive index with a second continuous probe laser beam (633 nm, 1.4 mW). We focus on intravital monitoring of the integral PT response from single, moving, unlabeled cells (from 100 to 500 cells in one measurement). Potential in vivo applications of this new optical tool, called PT flow cytometry (PTFC), are discussed, including identification of selected cells with differences in natural absorptive properties and sizes, determination of laser-induced cell damage, estimation of flow velocity, and monitoring of circulating cells labeled with PT probes.

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“…In fact, Starr et al [8] found that the blood flow in cat mesenteric microvessels in vivo is characterized by a discrepancy between RBC and plasma velocities. This discrepancy is dependent on the elastic properties of RBCs and flow dynamics, including plasma shear and pressure field.…”

Section: Study Of Microlymphatic Dynamicsmentioning

confidence: 99%

Advances in small animal mesentery models for in vivo flow cytometry, dynamic microscopy, and drug screening

Galanzha¹

2007

WJG

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Using animal mesentery with intravital optical microscopy is a well-established experimental model for studying blood and lymph microcirculation in vivo . Recent advances in cell biology and optical techniques provide the basis for extending this model for new applications, which should generate significantly improved experimental data. This review summarizes the achievements in this specific area, including in vivo label-free blood and lymph photothermal flow cytometry, super-sensitive fluorescence image cytometry, light scattering and speckle flow cytometry, microvessel dynamic microscopy, infrared (IR) angiography, and high-speed imaging of individual cells in fast flow. The capabilities of these techniques, using the rat mesentery model, were demonstrated in various studies; e.g., real-time quantitative detection of circulating and migrating individual blood and cancer cells, studies on vascular dynamics with a focus on lymphatics under normal conditions and under different interventions (e.g. lasers, drugs, nicotine), assessment of lymphatic disturbances from experimental lymphedema, monitoring cell traffic between blood and lymph systems, and high-speed imaging of cell transient deformability in flow. In particular, the obtained results demonstrated that individual cell transportation in living organisms depends on cell type (e.g., normal blood or leukemic cells), the cell's functional state (e.g., live, apoptotic, or necrotic), and the functional status of the organism. Possible future applications, including in vivo early diagnosis and prevention of disease, monitoring immune response and apoptosis, chemo-and radio-sensitivity tests, and drug screening, are also discussed. TOPIC HIGHLIGHT

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In vivo cellular and plasma velocities in microvessels of the cat mesentery

Cited by 17 publications

References 5 publications

Effects of Chronic Nicotine Administration on RBC Velocity in Mesenteric Capillaries of the Rat

Effects of Chronic Nicotine Administration on RBC Velocity in Mesenteric Capillaries of the Rat

Integrated photothermal flow cytometry in vivo

Advances in small animal mesentery models for in vivo flow cytometry, dynamic microscopy, and drug screening

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