2008
DOI: 10.1016/j.ultrasmedbio.2007.07.002
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In Vivo Cardiac Imaging of Adult Zebrafish Using High Frequency Ultrasound (45-75 MHz)

Abstract: The zebrafish has emerged as an excellent genetic model organism for studies of cardiovascular development. Optical transparency and external development during embryogenesis allow for visual analysis in the early development. However, to understand the cardiovascular structures and functions beyond the early stage requires a high-resolution, real-time, noninvasive imaging alternative due to the opacity of adult zebrafish. In this research, we report the development of a high frequency ultrasonic system for ad… Show more

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Cited by 97 publications
(80 citation statements)
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“…14,28 Under normal conditions most of the blood will fill the ventricle during early diastole in the human heart. Thus, the E-wave peak velocity is larger than the A-wave velocity, which means that the E/A ratio is larger than 1 for the normal human heart.…”
Section: Fig 5 Typical B-mode Image Of a Normal Zebrafish Heart Obtmentioning
confidence: 99%
See 2 more Smart Citations
“…14,28 Under normal conditions most of the blood will fill the ventricle during early diastole in the human heart. Thus, the E-wave peak velocity is larger than the A-wave velocity, which means that the E/A ratio is larger than 1 for the normal human heart.…”
Section: Fig 5 Typical B-mode Image Of a Normal Zebrafish Heart Obtmentioning
confidence: 99%
“…Although such an array system can provide a high imaging frame rate for the cardiac imaging of small animals, the use of higher frequency ultrasound would improve the image resolution. 14 The heart of the adult zebrafish is smaller than 1.5 · 1.5 · 1.5 mm 3,15 and so the ultrasound frequency needs to be higher than 75 MHz to provide sufficient resolution for imaging the detailed heart structures according to the suggestions from previous studies. 14,16 Array transducers operating at frequencies higher than 50 MHz are not available, but a singleelement transducer system based on mechanical scanning is still an option for zebrafish heart imaging.…”
Section: Introductionmentioning
confidence: 99%
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“…They are either optimised for diagnosing pathologies in humans (computed tomography (CT), magnetic resonance imaging (MRI), ultrasound (US), single photon emission computed tomography (SPECT), positron emission tomography (PET)), for in vivo analysis of -often transparent -model animals [23][24][25][26][27][28][29][30][31][32], or for post-mortem analyses of entire biological specimens and biological tissue samples, on the molecular, subcellular, cellular, tissue, and organ system level (atomic force microscopy, electron microscopy, confocal imaging, optical projection tomography (OPT), episcopic imaging techniques, histological sectioning, micro-MRI, micro-CT, near infrared imaging techniques, polarised light spectroscopy, optical coherence tomography (OCT)). Not all of these techniques permit 3D imaging of the developing cardiovascular system of unborn mice.…”
Section: Modern 3d Imagingmentioning
confidence: 99%
“…Recently, high-frequency ultrasonic imaging system has been used in the zebrafish heart study, and the results significantly improved delineation of detailed cardiac structures and accurate estimation of cardiac dimensions [4].…”
Section: Introductionmentioning
confidence: 99%