In vivo burn diagnosis by camera-phone diffuse reflectance laser speckle detection

Abstract

Burn diagnosis using laser speckle light typically employs widefield illumination of the burn region to produce two-dimensional speckle patterns from light backscattered from the entire irradiated tissue volume. Analysis of speckle contrast in these time-integrated patterns can then provide information on burn severity. Here, by contrast, we use point illumination to generate diffuse reflectance laser speckle patterns of the burn. By examining spatiotemporal fluctuations in these time-integrated patterns along the radial direction from the incident point beam, we show the ability to distinguish partial-thickness burns in a porcine model in vivo within the first 24 hours post-burn. Furthermore, our findings suggest that time-integrated diffuse reflectance laser speckle can be useful for monitoring burn healing over time post-burn. Unlike conventional diffuse reflectance laser speckle detection systems that utilize scientific or industrial-grade cameras, our system is designed with a camera-phone, demonstrating the potential for burn diagnosis with a simple imager.

Quantifying tissue perfusion after peripheral endovascular procedures: Novel tissue perfusion endpoints to improve outcomes

“…DSCA has found several applications in many fields since its development. Clinical applications include cerebral blood flow assessment[ 111 ], peripheral vascular health assessment for the evaluation of burns[ 112 ], and monitoring of foot tissue blood perfusion in diabetic patients with non-healing wound lesions undergoing vascular interventions[ 113 ].…”

Diffuse Speckle Contrast Analysis (DSCA) for Deep Tissue Blood Flow Monitoring

“…Another important application of DSCA can be found on periphery, as peripheral vascular health is important in treatment of either burn or diabetes. Burn-re-lated study was performed by [57]. For diabetic foot, which increases incessantly in developed countries, monitoring of blood perfusion on foot tissue with unhealing lesion is very important, especially when vascular surgeons perform balloon angioplasty in order to open up clogged arteries in the leg.…”