In vivo bone regenerative effect of low-intensity pulsed ultrasound in rat calvarial defects

Hasuike, Akira; Sato, Shuichi; Udagawa, Asami; Ando, Kazunari; Arai, Yoshinori; Ito, Koichi

doi:10.1016/j.tripleo.2010.09.061

Cited by 29 publications

(27 citation statements)

References 20 publications

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“…[7][8][9][10] Several studies explored potential treatments for OIIRR [11][12][13][14] but the only reported clinically acceptable modality is low-intensity pulsed ultrasound (LIPUS). 15 LIPUS is acoustic pressure waves that can stimulate a variety of cells including cementoblasts, 16 odontoblasts, 17 osteoblasts, 18 chondrocytes, 19 gingival cells, 20,21 and periodontal ligament cells. 22 Previous studies investigating the effects of LIPUS on OIRR have reported that LIPUS promotes cementogenesis by increasing the Alkaline phosphate (ALP) activity, 16,23 collagen-I synthesis, 16 and Runx-2 protein 16 in cementoblasts and inhibits cementoclastogenesis 24,25 In addition, a previous study 15 reported that LIPUS reduced the severity of OIRR due to tipping-type orthodontic tooth movement and promoted cementum repair.…”

Section: Introductionmentioning

confidence: 99%

Effect of low-intensity pulsed ultrasound on orthodontically induced root resorption caused by torque: A prospective, double-blind, controlled clinical trial

Raza

Major

Dederich

et al. 2015

The Angle Orthodontist

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Objectives: To evaluate the effects of low-intensity pulsed ultrasound (LIPUS) on orthodontically induced tooth root resorption caused by torque in human subjects. Materials and Methods: Ten healthy patients (12-35 years of age) who required extraction of all first premolars as a part of their routine orthodontic treatment were recruited. A 15u twist was applied in the arch wire using 0.019 3 0.025-inch TMA in a 0.022-inch bracket system (Synergy R) that produced a buccal root torque of approximately 5 N/mm at the bracket level. Using a split mouth design, randomization, and blinding, one side of the arch received LIPUS for 20 minutes per day for 4 weeks at an incident intensity of 30 mW/cm 2 of the transducers' surface area. The other side served as a self-control, which received a sham transducer. After 4 weeks, all first premolars were extracted and micro-computed tomographic analysis was performed on these extracted teeth. A linear mixed-model statistical analysis was used. Results: LIPUS-treated teeth showed significantly less total volume of resorption lacunae compared to control teeth by a mean difference of (0.54 6 0.09 mm 3 ) (P , .001) and percentage of root resorption by a mean difference of (0.33 6 0.05 mm 3 ) (P , .001). In addition, significantly fewer resorption lacunae were found on all root surfaces in the LIPUS group compared to the control except in the instance of the distal surface. Limitations: This study was performed on limited number of cases during a 4-week period. Conclusions: LIPUS minimizes root resorption when applied during torque tooth movement over a 4-week period. (Angle Orthod. 2016;86:550-557.)

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Section: Introductionmentioning

confidence: 99%

Effect of low-intensity pulsed ultrasound on orthodontically induced root resorption caused by torque: A prospective, double-blind, controlled clinical trial

Raza

Major

Dederich

et al. 2015

The Angle Orthodontist

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“…In the present study, considering the attenuation, the penetrative ability of LIPUS and the localization of the bone defect from the transducer, we used a setting of 5 MHz frequency 160 mW/cm 2 intensity of LIPUS in 5 mm critical size bone defects of rat calvarium. This setting varied from the condition of stimulating bone regeneration in the rat calvaria bone defects, which has been recently reported [5,20], yet agreed to be the safe usage and dosage of ultrasound reported by Warden, S.J., et al [21].…”

Section: Figurementioning

confidence: 85%

“…Bones in the maxillofacial area are flat and formed by intramembranous ossification whereas most bones in other parts of the body are long bones and produced by endochondral ossification [11]. It has been recently reported that LIPUS also stimulates bone regeneration in calvaria bone defects in rats [5,12]. Furthermore, some dentists started to apply LIPUS clinically to the bone augmentation site in the maxillofacial area, such as sinus floor augmentation in Japan.…”

Section: Open Accessmentioning

confidence: 99%

“…It has been reported that application of low-intensity pulsed ultrasound stimulation (LIPUS) promotes fracture healing and LIPUS has been clinically applied in the orthopaedic field [2,3], where positive effects of LIPUS on fracture healing of long bone were clearly concluded to reduce the time frame of clinical and radiographic healing by 38% [4]; however, effects of LIPUS on bone regeneration of flat bones in the cranial-maxillofacial area have not been abundantly documented although promotion of reossification ratio in rat noncritical-sized parietal bone defects by LIPUS or by low magnitude and high frequency mechanical stimuli has been recently reported [5,6] and although some clinicians have already performed LIPUS for bone augmentations in this area. Effects of LIPUS on bone regeneration in the cranial-maxillofacial should be carefully evaluated in animal experiments prior to its clinical trials.…”

Section: Introductionmentioning

confidence: 99%

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Effects of low intensity pulsed ultrasound stimulation on bone regeneration in rat parietal bone defect model

Chen¹,

Jia²,

Noritake³

et al. 2013

OJRM

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Purpose: Low intensity pulsed ultrasound stimulation (LIPUS) has been clinically applied to promote bone fracture healing in the orthopedic field. Thus, it is likely that LIPUS also stimulates bone regeneration in bone defects in the cranial-maxillofacial area. However, this has not been clearly proved. Furthermore, optimal time point and period of the application after the surgery has not been reported. The purpose of the present study was to evaluate the effect of LIPUS on bone regeneration in the rat parietal bone defects especially focusing on time and period of the application. Materials and Methods: Eighteen Wistar rats (14 weeks old) were divided into 6 groups: 5 experimental groups and a control group. Bone defect of 5 mm diameter was prepared on each side of the parietal bone and customized gelatin membranes were placed over the bone defects. LIPUS (160 mW/cm 2 , 15 min/day) was applied to the defect area with an active transducer externally in the experimental groups according to the schedules of the applications: Group 1 (day 6 -12), group 2 (day 13 -19), group 3 (day 20 -26), group 4 (day 6 -19) and group 5 (day 6 -26). All the animals were sacrificed at 28 days. The defects were analyzed with micro CT and then histologically. Results: In Group 1, new bone formation was significantly promoted and the newly-formed bone was thick and matured compared to the one of the control group. In other experimental groups there were tendencies of stimulation of new bone formation; however, they were not statistically significant. Discussion and Conclusion: The present study demonstrated that amount of new bone formation in the bone defect depended on the time and period of LIPUS application. It has been suggested that application of LIPUS at an early healing period, the second week after the surgery, effectively accelerated new bone formation.

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“…Low-intensity pulsed ultrasound (LIPUS) is a biophysical intervention used in the bone-repair process; typical applications use 1.6-MHz pulses, a 200-ìs pulse width, and 1-kHz repetitions for 20 min/day. LIPUS provides a very mild mechanical stimulation of bone, and its effect has been studied in vitro [5][6][7][8] , in animal models 9,10) , and in clinical settings 11,12) involving fractures and other healing responses. Our previous report used in vivo microfocus computed tomography (micro-CT) to demonstrate the bone regenerative effect of LIPUS treatment in non-critical 2.7-mm rat calvarial defects 9) .…”

Section: Introductionmentioning

confidence: 99%

The Potency of Low-Intensity Pulsed Ultrasound in a Rat Calvarial Guided Bone-Regeneration Model

Hasuike

Sato

Makino

et al. 2011

J. Hard Tissue Biology.

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Abstract:Low-intensity pulsed ultrasound (LIPUS) is a biophysical intervention used to repair bone. LIPUS may reduce the healing time of bone defects covered with osteoconductive membranes. The purpose of this study was to identify the required characteristics of membranes used with LIPUS in guided bone-regeneration (GBR) procedures. Two bioabsorbable membranes (Bio-Mend ® , GC membrane), one non-bioabsorbable membrane (GORE-TEX ® ), and one titanium membrane (FRIOS ® BoneShield) were evaluated with an oscilloscope in two settings: underwater and with an ultrasound gel. One side of the membrane was attached to the LIPUS transducer, and the other side was connected to the oscilloscope transducer. The commercial bioabsorbable and GORE-TEX ® membranes transmitted only 7-16% of the 30-and 60-mW/cm 2 LIPUS waves with ultrasound gel or while underwater, whereas the titanium membrane transmitted 81-90% of these waves in both settings. Based on results of propagation analysis, we also examined the potency of LIPUS used with GBR in critical bone defects in vivo in a rat calvarium model. Two 5.0-mm defects were trephined in rat calvarium and covered with a GC membrane and FRIOS ® BoneShield, respectively. Rats were exposed to LIPUS 30 min/day for 3 weeks. In vivo, more active bone regeneration was observed in defects covered with titanium membranes than in those covered with GC membrane in the combined use of LIPUS with GBR. These results suggest that titanium membranes perform better than other commercial membranes in the combined use of LIPUS with GBR.

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In vivo bone regenerative effect of low-intensity pulsed ultrasound in rat calvarial defects

Cited by 29 publications

References 20 publications

Effect of low-intensity pulsed ultrasound on orthodontically induced root resorption caused by torque: A prospective, double-blind, controlled clinical trial

Effect of low-intensity pulsed ultrasound on orthodontically induced root resorption caused by torque: A prospective, double-blind, controlled clinical trial

Effects of low intensity pulsed ultrasound stimulation on bone regeneration in rat parietal bone defect model

The Potency of Low-Intensity Pulsed Ultrasound in a Rat Calvarial Guided Bone-Regeneration Model

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