In vivo bone formation by human marrow stromal cells in biodegradable scaffolds that release dexamethasone and ascorbate-2-phosphate

Kim, Hyongbum; Suh, Hwal; Jo, Sangmee Ahn; Kim, Hyun Woo; Lee, Jung Min; Kim, Eun Hae; Reinwald, Yvonne; Park, Sang Hyug; Min, Byoung Hyun; Jo, Inho

doi:10.1016/j.bbrc.2005.05.051

Cited by 83 publications

(43 citation statements)

References 49 publications

(55 reference statements)

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“…Bone marrow stromal cells (hBMSCs) were isolated as described previously [22] and were plated in T75 flasks for continuous passaging in DMEM medium supplemented with 20% FBS (Gibco) and 1% antibiotic-antimycotic solution (Gibco). Medium was changed twice weekly and cells detached by trypsin-EDTA and passaged into fresh culture flasks at a ratio of 1:4 upon reaching confluence.…”

Section: Matrix Mineralization Of Cultured Bone Marrow Stromal Cells mentioning

confidence: 99%

Assembly of collagen-binding peptide with collagen as a bioactive scaffold for osteogenesis in vitro and in vivo

et al. 2007

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Section: Matrix Mineralization Of Cultured Bone Marrow Stromal Cells mentioning

confidence: 99%

Assembly of collagen-binding peptide with collagen as a bioactive scaffold for osteogenesis in vitro and in vivo

et al. 2007

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“…Large doses of exogenous growth factors may cause unexpected physiological effects ranging from bone resorption (Giannoudis et al, 2007) to heterotopic ossification (Wysocki and Cohen, 2007;Axelrad et al, 2008). Hence, alternative methods for effective osteoinduction of MSCs are under investigation Kim et al, 2005;McCullen et al, 2010;Tirkkonen et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Osteogenic medium is superior to growth factors in differentiation of human adipose stem cells towards bone-forming cells in 3D culture

Tirkkonen¹,

Haimi²,

Huttunen³

et al. 2013

eCM

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Human adipose stem cells (hASCs) have been recently used to treat bone defects in clinical practice. Yet there is a need for more optimal scaffolds and cost-effective approaches to induce osteogenic differentiation of hASCs. Therefore, we compared the efficiency of bone morphogenetic proteins (BMP-2 and BMP-7), vascular endothelial growth factor (VEGF), and osteogenic medium (OM) for the osteoinduction of hASCs in 3D culture. In addition, growth factors were tested in combination with OM. Commercially available bioactive glass scaffolds (BioRestore) and biphasic calcium phosphate granules (BoneCeramic) were evaluated as prospective carriers for hASCs. Both biomaterials supported hASC-viability, but BioRestore resulted in higher cell number than BoneCeramic, whereas BoneCeramic supported more significant collagen production. The most efficient osteo-induction was achieved with plain OM, promoting higher alkaline phosphatase activity and collagen production than growth factors. In fact, treatment with BMP-2 or VEGF did not increase osteogenic differentiation or cell number significantly more than maintenance medium with either biomaterial. Moreover, BMP-7 treatment consistently inhibited proliferation and osteogenic differentiation of hASCs. Interestingly, there was no benefit from growth factors added to OM. This is the first study to demonstrate that OM enhances hASCdifferentiation towards bone-forming cells significantly more than growth factors in 3D culture.

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“…MSCs are multipotent cells that can replicate as undifferentiated cells with the potential to differentiate and produce mesenchymal tissue [35]. MSC-based bone tissue engineering has been studied as a therapy for bone defects, and MSCs must differentiate into osteoblasts for the formation of bone tissue [36].…”

Section: Cytotoxic Test Of Porous Scaffoldsmentioning

confidence: 99%

In vivo biocompatibility and mechanical properties of porous zein scaffolds

et al. 2007

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In vivo bone formation by human marrow stromal cells in biodegradable scaffolds that release dexamethasone and ascorbate-2-phosphate

Cited by 83 publications

References 49 publications

Assembly of collagen-binding peptide with collagen as a bioactive scaffold for osteogenesis in vitro and in vivo

Assembly of collagen-binding peptide with collagen as a bioactive scaffold for osteogenesis in vitro and in vivo

Osteogenic medium is superior to growth factors in differentiation of human adipose stem cells towards bone-forming cells in 3D culture

In vivo biocompatibility and mechanical properties of porous zein scaffolds

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