In vivo bone formation by and inflammatory response to resorbable polymer-nanoclay constructs

Baker, Kevin C.; Maerz, Tristan; Saad, H. M.; Shaheen, Philip E.; Kannan, Rangaramanujam M.

doi:10.1016/j.nano.2015.06.012

Cited by 11 publications

(7 citation statements)

References 64 publications

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“…Here, we studied CLN/PCEC scaffolds in a unicortical tibial defect model, and CLN was shown to induce bone regeneration in vivo for the first time (histological scores can be seen in Table S1). In a similar study by Baker, Maerz, Saad, Shaheen, and Kannan (), MNT was studied in a poly( d ‐lactic acid)‐based composite scaffold for bone regeneration capacity (Baker et al, ). Although MNT was shown to support bone growth, it was observed that growth factor and/or HA presence was required to achieve osteogenesis.…”

Section: Resultsmentioning

confidence: 99%

Composite clinoptilolite/PCL‐PEG‐PCL scaffolds for bone regeneration: In vitro and in vivo evaluation

Pazarçeviren

Dikmen

Altunbaş

et al. 2019

J Tissue Eng Regen Med

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In this study, clinoptilolite (CLN) was employed as a reinforcement in a polymer-based composite scaffold in bone tissue engineering and evaluated in vivo for the first time.Highly porous, mechanically stable, and osteogenic CLN/PCL-PEG-PCL (CLN/PCEC) scaffolds were fabricated with modified particulate leaching/compression molding technique with varying CLN contents. We hypothesized that CLN reinforcement in a composite scaffold will improve bone regeneration and promote repair. Therefore, the scaffolds were analyzed for compressive strength, biodegradation, biocompatibility, and induction of osteogenic differentiation in vitro. CLN inclusion in PC-10 (10% w/w) and PC-20 (20% w/w) scaffolds revealed 54.7% and 53.4% porosity, higher dry (0.62 and 0.76 MPa), and wet (0.37 and 0.45 MPa) compressive strength, greater cellular adhesion, alkaline phosphatase activity (2.20 and 2.82 mg/g DNA /min), and intracellular calcium concentration (122.44 and 243.24 g Ca/mg DNA ). The scaffolds were evaluated in a unicortical bone defect at anterior aspect of proximal tibia of adult rabbits 4 and 8 weeks postimplantation. Similar to in vitro results, CLN-containing scaffolds led to efficient regeneration of bone in a dose-dependent manner. PC-20 demonstrated highest quality of bone union, cortex development,and bone-scaffold interaction at the defect site. Therefore, higher CLN content in PC-20 permitted robust remodeling whereas pure PCEC (PC-0) scaffolds displayed fibrous tissue formation. Consequently, CLN was proven to be a potent reinforcement in terms of promoting mechanical, physical, and biological properties of polymer-based scaffolds in a more economical, easy-to-handle, and reproducible approach.

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Section: Resultsmentioning

confidence: 99%

Composite clinoptilolite/PCL‐PEG‐PCL scaffolds for bone regeneration: In vitro and in vivo evaluation

Pazarçeviren

Dikmen

Altunbaş

et al. 2019

J Tissue Eng Regen Med

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“…Notably, the authors observed a higher bone volume in PDLA‐93A‐BMP‐2 compared to PDLA‐BMP‐2 scaffolds after 6 weeks. [ 87 ] Likewise, Kapusetti et al. performed a study to show the ability of polymeric bone cement (BC) reinforced with MMT to regenerate bone in a rabbit tibia model.…”

Section: In Vivo Studiesmentioning

confidence: 99%

“…Although the results of these studies are generally positive, the outcomes here vary greatly (Table 1); with a lot of diversity in the conclusions. Regarding possible complications in a postimplantation scenario, some studies have reported inflammatory reactions, [ 87 ] but even still, the majority still demonstrate that nanoclay reinforced hydrogels or scaffolds are biocompatible. [ 61,72,66 ] Almost all studies highlight that the inclusion of nanoclays improves mechanical and even biological in vitro properties of created systems, but there is still a gap when it comes down to really assessing what role nanomaterials play .…”

Section: In Vivo Studiesmentioning

confidence: 99%

“…Therefore, multidisciplinary research efforts have been directed toward developing tissue‐engineered constructs that could display sufficient mechanical properties, bioactivity, and resorbability. [ 6,91–104 ] Amongst the different materials explored in this direction, nanoclay reinforced materials have recently opened new perspectives for T/L and cartilage repair [ 24,32,87,92,97,104–113 ] mainly due to their improved mechanical performance and capacity to stimulate new tissue formation. For example, in vivo studies using a rat‐based artificial cartilage‐ligament reconstruction model observed that unidirectional‐braided silk‐based hybrid fibers with bioactive nanoclay Laponite RD displayed better cartilage remodeling compared with pristine counterparts ( Figure ).…”

Section: In Vivo Studiesmentioning

confidence: 99%

“…Despite these encouraging results, there are unfortunately still some studies that show a certain degree of toxicity. Baker et al, for example, excluded scaffolds containing Cloisite 30B (modified MMT) because of their inflammatory response, [ 87 ] and Boyer et al. observed that Laponite RD prepared as a suspension can show some toxicity at concentrations around and above 10 µg L −1 .…”

Section: In Vivo Studiesmentioning

confidence: 99%

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Nanoclay‐reinforced biomaterials have sparked a new avenue in advanced healthcare materials that can potentially revolutionize treatment of musculoskeletal defects. Native tissues display many important chemical, mechanical, biological, and physical properties that engineered biomaterials need to mimic for optimal tissue integration and regeneration. However, it is time‐consuming and difficult to endow such combinatorial properties on materials via feasible and nontoxic procedures. Fortunately, a number of nanomaterials such as graphene, carbon nanotubes, MXenes, and nanoclays already display a plethora of material properties that can be transferred to biomaterials through a simple incorporation procedure. In this direction, the members of the nanoclay family are easy to functionalize chemically, they can significantly reinforce the mechanical performance of biomaterials, and can provide bioactive properties by ionic dissolution products to upregulate cartilage and bone tissue formation. For this reason, nanoclays can become a key component for future orthopedic biomaterials. In this review, we specifically focus on the rapidly decreasing gap between clinic and laboratory by highlighting their application in a number of promising in vivo studies.

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Multi-factor Optimization for Joining of Polylactic Acid-Hydroxyapatite-Chitosan Based Scaffolds by Rapid Joining Process

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Singh

Ahuja

2022

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In vivo bone formation by and inflammatory response to resorbable polymer-nanoclay constructs

Cited by 11 publications

References 64 publications

Composite clinoptilolite/PCL‐PEG‐PCL scaffolds for bone regeneration: In vitro and in vivo evaluation

Composite clinoptilolite/PCL‐PEG‐PCL scaffolds for bone regeneration: In vitro and in vivo evaluation

Nanoclay Reinforced Biomaterials for Mending Musculoskeletal Tissue Disorders

Multi-factor Optimization for Joining of Polylactic Acid-Hydroxyapatite-Chitosan Based Scaffolds by Rapid Joining Process

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