In vivo biodistribution of stable spherical and filamentous micelles probed by high-sensitivity SPECT

Jennings, Laurence; Ivashchenko, Oleksandra; Marsman, I. J. C.; Laan, A.C.; Denkova, Antonia G.; Waton, Gilles; Beekman, Freek J.; Schosseler, F.; Mendes, Eduardo

doi:10.1039/c6bm00297h

Cited by 11 publications

(5 citation statements)

References 43 publications

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“…In this respect, calcium carbonate may be useful due to its biocompatibility and low cost. Particle biophysical properties affect drug biodistribution, selective targeting, and elimination from the body [23][24][25]. Therefore, extensive efforts have been invested in controlling the fabrication of particles, either as drug carriers or as templates for LbL formulations.…”

Section: Discussionmentioning

confidence: 99%

Controlling Calcium Carbonate Particle Morphology, Size, and Molecular Order Using Silicate

et al. 2021

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Calcium carbonate (CaCO3) is one of the most abundant substances on earth and has a large array of industrial applications. Considerable research has been conducted in an effort to synthesize calcium carbonate microparticles with controllable and specific morphologies and sizes. CaCO3 produced by a precipitation reaction of calcium nitrate and sodium carbonate solution was found to have high polymorphism and batch to batch variability. In this study, we investigated the polymorphism of the precipitated material and analyzed the chemical composition, particle morphology, and crystalline state revealing that the presence of silicon atoms in the precipitant is a key factor effecting particle shape and crystal state. An elemental analysis of single particles within a polymorphic sample, using energy-dispersive X-ray spectroscopy (EDS) conjugated microscopy, showed that only spherical particles, but not irregular shaped one, contained traces of silicon atoms. In agreement, silicon-containing additives lead to homogenous, amorphous nanosphere particles, verified by X-ray powder diffraction (XRD). Our findings provide important insights into the mechanism of calcium carbonate synthesis, as well as introducing a method to control the precipitants at the micro-scale for many diverse applications.

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Section: Discussionmentioning

confidence: 99%

Controlling Calcium Carbonate Particle Morphology, Size, and Molecular Order Using Silicate

et al. 2021

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“…Block copolymer micelles were prepared using an evaporation-induced self-assembly method, which you can find elsewhere. , Briefly, block copolymers were completely dissolved in chloroform to make the stock solutions with concentration of 10 mg/mL. To prepare the micelles with MA groups, PS- b -PEO-MA was mixed with PS- b -PEO at certain weight percentages and the corresponding samples were labeled as PS- b -PEO-MA micelles.…”

Section: Experimental Sectionmentioning

confidence: 99%

Two Robust Strategies toward Hydrogels from Quenched Block Copolymer Nanofibrillar Micelles

et al. 2018

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While the formation of (tri)block copolymer hydrogels has been extensively investigated, such studies mostly focused on equilibrium self-assembling whereas the use of preformed structures as building blocks such as out of equilibrium, quenched, nanofibrillar micelles is still a challenge. Here, we demonstrate that quenched, ultralong polystyrene-b-poly(ethylene oxide) (PS-b-PEO) micelles can be used as robust precursors of hydrogels. Two cross-linking strategies, (i) thermal fusion of micellar cores and (ii) chemical cross-linking of preformed micellar coronas were studied. The gelation process and the structure of the micellar networks were investigated by in situ rheological measurements, confocal microscopy and transmission electron microscopy. Direct observation of core fusion of preformed quenched micelles is provided validating this method as a robust gelation route. Using time sweep rheological experiments, it was found for both cross-linking methods that these 3D “mikado” gels are formed in three different stages, containing (1) initiation, (2) transition (growth), and (3) stabilization regimes.

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“…The self-assembly polymeric micelles are designed to prolong the circulation period in blood, possess tumor targeting capability, and improve drug therapies, and also reduce the side effects and increase the desired effects of drugs. 4,5 For these controlled drug delivery carriers, 6 most triggers are responsive in pH, 7,8 light, 9 temperature, 10 ultrasound, 11 and redox environments. [12][13][14] Despite the fact that a stimuli-triggered release system has greatly improved drug therapies, low drug loading content (DLC) of polymeric micelles and slow accumulation of drug carriers still limit the effectiveness of chemotherapy, leading consequently to low drug concentration on the cell surface, which reduces the effect of anti-tumor treatment.…”

Section: Introductionmentioning

confidence: 99%

High drug loading and pH-responsive targeted nanocarriers from alginate-modified SPIONs for anti-tumor chemotherapy

Peng

Wang

et al. 2016

Biomater. Sci.

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To increase the accumulation of nanocarriers at the tumor site and reduce premature drug leakage, we fabricated alginate modified superparamagnetic iron oxide nanoparticles (SPIONs) with magnetic targeting capability for pH-responsive release of the anticancer drug doxorubicin (DOX) in tumor-cell microenvironments. The drug loading content (DLC) of SPION-4 was as high as 48.98% with a stable size of 135 nm, whereas the DLC of SPION-2 (amine-functionalized SPIONs as the control) was 7.58%. The in vitro release studies revealed that the acidic environment (pH 6.5 and pH 5.0) triggered the effective release of DOX from DOX-loaded SPION-4 twice and thrice as much as the neutral condition (pH 7.4) after 10.7 h, respectively. These nanocarriers exhibited good cytocompatibility towards both normal cells (LO2) and cancer cells (HepG2), but higher cytotoxicity against HepG2 cells for DOX-loaded SPION-4 than that against LO2 cells could be observed due to the effects of an additional magnet and the acidic microenvironment of cancer cells, which greatly improved the cellular uptake of magnetic nanoparticles and released more DOX in the cytoplasm and nucleus. The in vivo biodistribution and anti-tumor efficacy demonstrated that DOX-loaded SPION-4 under an external magnetic field could obviously increase the DOX concentration in tumor tissues to remarkably inhibit tumor growth and significantly reduce side effects of free DOX. Therefore, this work suggested that these SPION-4 nanoparticles may be a potential carrier to deliver chemotherapeutic agents in anti-tumor treatment.

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In vivo biodistribution of stable spherical and filamentous micelles probed by high-sensitivity SPECT

Cited by 11 publications

References 43 publications

Controlling Calcium Carbonate Particle Morphology, Size, and Molecular Order Using Silicate

Controlling Calcium Carbonate Particle Morphology, Size, and Molecular Order Using Silicate

Two Robust Strategies toward Hydrogels from Quenched Block Copolymer Nanofibrillar Micelles

High drug loading and pH-responsive targeted nanocarriers from alginate-modified SPIONs for anti-tumor chemotherapy

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