In vivo biocompatibility of ultra-short single-walled carbon nanotube/biodegradable polymer nanocomposites for bone tissue engineering

Sitharaman, Balaji; Shi, Xinfeng; Walboomers, X. Frank; Liao, Hongyu; Cuijpers, Vincent M.J.I.; Wilson, Lon J.; Mikos, Antonios G.; Jansen, John A.

doi:10.1016/j.bone.2008.04.013

Cited by 236 publications

(171 citation statements)

References 43 publications

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“…Meng et al (10) reported improved fibroblast growth and collagen synthesis on a nanofibrous scaffold made of MWCNT/polyurethane compared to that of controls and polyurethane alone. Sitharaman et al (11) confirmed that the in vivo tissue response of ultra-short SWCNT/poly(propylene fumarate) and poly(propylene fumarate diacrylate) composite scaffolds was similar to that of the polymers alone.…”

supporting

confidence: 57%

In vitro studies of multiwalled carbon nanotube/ultrahigh molecular weight polyethylene nanocomposites with osteoblast-like MG63 cells

Reis

Kanagaraj

Fonseca

et al. 2010

Braz J Med Biol Res

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Carbon nanotubes are highly versatile materials; new applications using them are continuously being developed. Special attention is being dedicated to the possible use of multiwalled carbon nanotubes in biomaterials contacting with bone. However, carbon nanotubes are also controversial in regards to effects exerted on living organisms. Carbon nanotubes can be used to improve the tribological properties of polymer/composite materials. Ultrahigh molecular weight polyethylene (UHMWPE) is a polymer widely used in orthopedic applications that imply wear and particle generation. We describe here the response of human osteoblast-like MG63 cells after 6 days of culture in contact with artificially generated particles from both UHMWPE polymer and multiwalled carbon nanotubes (MWCNT)/UHMWPE nanocomposites. This novel composite has superior wear behavior, having thus the potential to reduce the number of revision hip arthroplasty surgeries required by wear failure of acetabular cups and diminish particle-induced osteolysis. The results of an in vitro study of viability and proliferation and interleukin-6 (IL-6) production suggest good cytocompatibility, similar to that of conventional UHMWPE (WST-1 assay results are reported as percentage of control ± SD: UHMWPE = 96.19 ± 7.92, MWCNT/UHMWPE = 97.92 ± 8.29%; total protein: control = 139.73 ± 10.78, UHMWPE = 137.07 ± 6.17, MWCNT/UHMWPE = 163.29 ± 11.81 µg/mL; IL-6: control = 90.93 ± 10.30, UHMWPE = 92.52 ± 11.02, MWCNT/UHMWPE = 108.99 ± 9.90 pg/mL). Standard cell culture conditions were considered as control. These results, especially the absence of significant elevation in the osteolysis inductor IL-6 values, reinforce the potential of this superior wearresistant composite for future orthopedic applications, when compared to traditional UHMWPE.

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supporting

confidence: 57%

In vitro studies of multiwalled carbon nanotube/ultrahigh molecular weight polyethylene nanocomposites with osteoblast-like MG63 cells

Reis

Kanagaraj

Fonseca

et al. 2010

Braz J Med Biol Res

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“…17 Considering their excellent mechanical properties, cytocompatibility, and electrical properties, they have received a lot of attention for bone tissue engineering. [18][19][20][21][22][23] Their nanofibrous geometry also simulates the extracellular matrix in bone. Carbon nanotubes can be synthesized through arc discharge, laser ablation, and chemical vapor deposition.…”

Section: Introductionmentioning

confidence: 99%

Biomimetic three-dimensional nanocrystalline hydroxyapatite and magnetically synthesized single-walled carbon nanotube chitosan nanocomposite for bone regeneration

Li²,

Wang³

et al. 2012

IJN

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Background: Many shortcomings exist in the traditional methods of treating bone defects, such as donor tissue shortages for autografts and disease transmission for allografts. The objective of this study was to design a novel three-dimensional nanostructured bone substitute based on magnetically synthesized single-walled carbon nanotubes (SWCNT), biomimetic hydrothermally treated nanocrystalline hydroxyapatite, and a biocompatible hydrogel (chitosan). Both nanocrystalline hydroxyapatite and SWCNT have a biomimetic nanostructure, excellent osteoconductivity, and high potential to improve the load-bearing capacity of hydrogels. Methods: Specifically, three-dimensional porous chitosan scaffolds with different concentrations of nanocrystalline hydroxyapatite and SWCNT were created to support the growth of human osteoblasts (bone-forming cells) using a lyophilization procedure. Two types of SWCNT were synthesized in an arc discharge with a magnetic field (B-SWCNT) and without a magnetic field (N-SWCNT) for improving bone regeneration. Results: Nanocomposites containing magnetically synthesized B-SWCNT had superior cytocompatibility properties when compared with nonmagnetically synthesized N-SWCNT. B-SWCNT have much smaller diameters and are twice as long as their nonmagnetically prepared counterparts, indicating that the dimensions of carbon nanotubes can have a substantial effect on osteoblast attachment. Conclusion: This study demonstrated that a chitosan nanocomposite with both B-SWCNT and 20% nanocrystalline hydroxyapatite could achieve a higher osteoblast density when compared with the other experimental groups, thus making this nanocomposite promising for further exploration for bone regeneration.

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“…These recent studies stress the need of careful re-evaluation and research before enlarging the field of CNT application. However, as Fraczek et al, Lacerda et al, Schipper et al and Sitharaman et al, among others, findings suggest, CNT behaviour in vivo depends on their length, functionalization and degree of agglutination (Fraczek et al, 2008;Lacerda et al, 2008;Schipper et al, 2008;Sitharaman et al, 2008). Up to now, several studies report low or undetected liver and systemic toxicity in mice, although CNT presence has been shown in the liver, lung and faeces after intravenous injection (Deng et al, 2007;Fraczek et al, 2008;Schipper et al, 2008).…”

Section: Biological Response and Mechanisms Of Toxicitymentioning

confidence: 96%

Carbon Nanotubes – Interactions with Biological Systems

Reis¹,

Capela-Silva²,

Potes³

et al. 2011

Carbon Nanotubes - Growth and Applications

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In vivo biocompatibility of ultra-short single-walled carbon nanotube/biodegradable polymer nanocomposites for bone tissue engineering

Cited by 236 publications

References 43 publications

In vitro studies of multiwalled carbon nanotube/ultrahigh molecular weight polyethylene nanocomposites with osteoblast-like MG63 cells

In vitro studies of multiwalled carbon nanotube/ultrahigh molecular weight polyethylene nanocomposites with osteoblast-like MG63 cells

Biomimetic three-dimensional nanocrystalline hydroxyapatite and magnetically synthesized single-walled carbon nanotube chitosan nanocomposite for bone regeneration

Carbon Nanotubes – Interactions with Biological Systems

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