In vivo behavior of complete human oral mucosa equivalents: characterization in athymic mice

Abstract

The present study shows the potential regenerative capacity of the CAOMEs by their ability to reach maturity similar to that seen in oral mucosa.

Sequential keratinocytic differentiation and maturation in a three‐dimensional model of human artificial oral mucosa

“…In our work, we employed a fibrin-agarose scaffold that overcomes the drawbacks of both the animal-derived and the synthetic biomaterials used in other studies, such as the biocompatibility, biodegradability, fibroblastic infiltration capacity, morphologic and mechanical stability, and feasibility of an autologous source (17). Moreover, in comparison with other fibrin-based scaffolds previously reported (2,3,20), the fibrin-agarose improves the rheological stability while maintaining the viscoelastic properties of the native tissue (21). The construction of a human oral mucosa substitute intended for clinical application should ideally employ cells and biomaterials derived from the patient to avoid immunological concerns.…”

“…The epithelial layer plays a major function as a barrier against external aggressions such a physical damage, bacterial infection, heat loss and ionizing radiation. The epithelial tissue consists of several layers of keratinocyte cells undergoing constant differentiation and self‐renewal, and recent studies focused on the role that key proteins and biological regulators such as cytokeratins, basement membrane proteins and intercellular junction proteins play in this process . However, these studies have not been carried out on artificial oral mucosa (AOM) substitutes developed by tissue engineering.…”

Tissue-engineered Oral Mucosa

“…In vivo animal studies have been carried out to investigate the role of grafted tissue-engineered oral mucosa in wound healing. Pena et al (2011) evaluated an autologous fibrin glue-based engineered oral mucosa by subcutaneous implantation in athymic mice. Although the results of their study showed that the epithelium remained viable and expressed a cytokeratin profile similar to that of native oral mucosa, it may be difficult to extrapolate the data to the clinical situation, where wounds are left open and the grafted material is air-exposed.…”

Tissue-Engineered Oral Mucosa for Mucosal Reconstruction in a Pediatric Patient with Hemifacial Microsomia and Ankyloglossia

“…Fibrin is an ideal matrix because it can stimulate fibroblast proliferation while preserving the epidermal cell “sternness” (Del Rio et al, 2002). Once it is transplanted, fibrin matrix is replaced by collagen fibers synthesized de novo by the embedded fibroblast population and forms tissue histologically similar to a natural lamina propria (Peña et al, 2011).…”