In Vivo Bacteriophage Peptide Display to Tailor Pharmacokinetics of Biological Nanoparticles

Dickerson, Marie T.; Kumar, Sudeep; Smith, George P.; Quinn, Thomas P.; Deutscher, Susan L.

doi:10.1007/s11307-014-0762-z

Cited by 10 publications

(9 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Another investigation that also studied kidney targeting in vivo found that peptide specificity could be used for the tailoring of pharmacokinetics. 378 By directing clearance toward the kidneys and away from the reticuloendothelial system, more rapid clearance could be achieved. In vivo screening has also been applied in humans for the mapping of unique zip codes lining the vasculature.…”

Section: Biotechnologymentioning

confidence: 99%

Design of virus-based nanomaterials for medicine, biotechnology, and energy

2016

View full text Add to dashboard Cite

Virus-based nanomaterials are versatile materials that naturally self-assemble and have relevance for a broad range of applications including medicine, biotechnology, and energy. This review provides an overview of recent developments in “chemical virology.” Viruses, as materials, provide unique nanoscale scaffolds that have relevance in chemical biology and nanotechnology, with diverse areas of applications. Some fundamental advantages of viruses, compared to synthetically programmed materials, include the highly precise spatial arrangement of their subunits into a diverse array of shapes and sizes and many available avenues for easy and reproducible modification. Here, we will first survey the broad distribution of viruses and various methods for producing virus-based nanoparticles, as well as engineering principles used to impart new functionalities. We will then examine the broad range of applications and implications of virus-based materials, focusing on the medical, biotechnology, and energy sectors. We anticipate that this field will continue to evolve and grow, with exciting new possibilities stemming from advancements in the rational design of virus-based nanomaterials.

show abstract

Section: Biotechnologymentioning

confidence: 99%

Design of virus-based nanomaterials for medicine, biotechnology, and energy

2016

View full text Add to dashboard Cite

show abstract

“…The phage display technology described here allowed us to examine polypeptides that specifically bind to tumors. It could rapidly screen bioactive peptides, proteins, receptors, and other novel or lead compounds with high stability and specificity in the body [ 15 , 16 ]. However, the phage display technology has a limited ability to detect in vivo and in vitro differences in polypeptide metabolism or differences in affinity and clearance between polypeptides and targeted proteins.…”

Section: Discussionmentioning

confidence: 99%

Radiation-Guided Peptide Delivery in a Mouse Model of Nasopharyngeal Carcinoma

Lin

et al. 2016

BioMed Research International

View full text Add to dashboard Cite

Purpose. This study aimed to evaluate the characteristics of the HVGGSSV peptide, exploring radiation-guided delivery in a mouse model of nasopharyngeal carcinoma. Methods. Mice with CNE-1 nasopharyngeal carcinoma were assigned to two different groups treated with Cy7-NHS and Cy7-HVGGSSV, respectively. Meanwhile, each mouse received a single dose of 3 Gy radiation. Biological distribution of the recombinant peptide was assessed on an in vivo small animal imaging system. Results. The experimental group showed maximum fluorescence intensity in irradiated tumors treated with Cy7-labeled HVGGSSV, while untreated (0 Gy) control tumors showed lower intensity levels. Fluorescence intensities of tumors in the right hind limbs of experimental animals were 7.84 × 107 ± 1.13 × 107, 1.35 × 108 ± 2.66 × 107, 4.05 × 108 ± 1.75 × 107, 5.57 × 108 ± 3.47 × 107, and 9.26 × 107 ± 1.73 × 107 photons/s/cm2 higher compared with left hind limb values at 1, 2, 15, 24, and 48 h, respectively. Fluorescence intensities of tumor in the right hind limbs of the experimental group were 1.66 × 108 ± 1.71 × 107, 1.51 × 108 ± 3.23 × 107, 5.38 × 108 ± 1.96 × 107, 5.89 × 108 ± 3.57 × 107, and 1.62 × 108 ± 1.69 × 107 photons/s/cm2 higher compared with control group values at 1, 2, 15, 24, and 48 h, respectively. Fluorescence was not specifically distributed in the control group. Compared with low fluorescence intensity in the heart, lungs, and tumors, high fluorescence distribution was found in the liver and kidney at 48 h. Conclusions. HVGGSSV was selectively bound to irradiated nasopharyngeal carcinoma, acting as a targeting transport carrier for radiation-guided drugs that are mainly metabolized in the kidney and liver.

show abstract

“…This methodology has produced mixed results [ 74 , 75 , 76 ]. Thus, for the past ~20 years in vivo phage display selections have been more widely utilized to select for peptides that optimally target tissues and organs within the milieu of a live animal [ 77 , 78 , 79 , 80 , 81 ]. More recently, in vivo phage selections have been performed to identify peptides with the capability of modifying biodistribution and clearance of themselves and/or larger particles [ 78 , 82 , 83 , 84 ].…”

Section: Principles Of Phage Displaymentioning

confidence: 99%

Improving Pharmacokinetics of Peptides Using Phage Display

Asar,

Newton-Northup,

Soendergaard

2024

Viruses

View full text Add to dashboard Cite

Phage display is a versatile method often used in the discovery of peptides that targets disease-related biomarkers. A major advantage of this technology is the ease and cost efficiency of affinity selection, also known as biopanning, to identify novel peptides. While it is relatively straightforward to identify peptides with optimal binding affinity, the pharmacokinetics of the selected peptides often prove to be suboptimal. Therefore, careful consideration of the experimental conditions, including the choice of using in vitro, in situ, or in vivo affinity selections, is essential in generating peptides with high affinity and specificity that also demonstrate desirable pharmacokinetics. Specifically, in vivo biopanning, or the combination of in vitro, in situ, and in vivo affinity selections, has been proven to influence the biodistribution and clearance of peptides and peptide-conjugated nanoparticles. Additionally, the marked difference in properties between peptides and nanoparticles must be considered. While peptide biodistribution depends primarily on physiochemical properties and can be modified by amino acid modifications, the size and shape of nanoparticles also affect both absorption and distribution. Thus, optimization of the desired pharmacokinetic properties should be an important consideration in biopanning strategies to enable the selection of peptides and peptide-conjugated nanoparticles that effectively target biomarkers in vivo.

show abstract

In Vivo Bacteriophage Peptide Display to Tailor Pharmacokinetics of Biological Nanoparticles

Abstract: In vivo phage display can identify phage and corresponding peptides that rapidly clear the renal system. In the future, these peptides may be used to impart favorable pharmacokinetics onto a wide range of radioimaging or therapeutic macromolecules.

Cited by 10 publications

References 31 publications

Design of virus-based nanomaterials for medicine, biotechnology, and energy

Design of virus-based nanomaterials for medicine, biotechnology, and energy

Radiation-Guided Peptide Delivery in a Mouse Model of Nasopharyngeal Carcinoma

Improving Pharmacokinetics of Peptides Using Phage Display

Contact Info

Product

Resources

About