In Vivo Assessment of VCAM-1 Expression by SPECT/CT Imaging in Mice Models of Human Triple Negative Breast Cancer

Abstract

Recent progress in breast cancer research has led to the identification of Vascular Cell Adhesion Molecule-1 (VCAM-1) as a key actor of metastatic colonization. VCAM-1 promotes lung-metastases and is associated with clinical early recurrence and poor outcome in triple negative breast cancer (TNBC). Our objective was to perform the in vivo imaging of VCAM-1 in mice models of TNBC. The Cancer Genomic Atlas (TCGA) database was analyzed to evaluate the prognostic role of VCAM-1 in TNBC. MDA-MB-231 (VCAM-1+) and control HCC70 (VCAM-1-) TNBC cells were subcutaneously xenografted in mice and VCAM-1 expression was assessed in vivo by single-photon emission computed tomography (SPECT) imaging using 99mTc-cAbVCAM1-5. Then, MDA-MB-231 cells were intravenously injected in mice and VCAM-1 expression in lung metastasis was assessed by SPECT imaging after 8 weeks. TCGA analysis showed that VCAM-1 is associated with a poor prognosis in TNBC patients. In subcutaneous tumor models, 99mTc-cAbVCAM1-5 uptake was 2-fold higher in MDA-MB-231 than in HCC70 (p < 0.01), and 4-fold higher than that of the irrelevant control (p < 0.01). Moreover, 99mTc-cAbVCAM1-5 uptake in MDA-MB-231 lung metastases was also higher than that of 99mTc-Ctl (p < 0.05). 99mTc-cAbVCAM1-5 is therefore a suitable tool to evaluate the role of VCAM-1 as a marker of tumor aggressiveness of TNBC.

Proteomics Identification and Validation of Desmocollin‐1 and Catechol‐O‐Methyltransferase as Proteins Associated with Breast Cancer Cell Migration and Metastasis

“…Identification of cell surfaceome provides a proteomic fingerprint representing breast cancer aggressiveness, which is accompanied by the migration of triple negative MDA‐MB‐231 breast cancer cells. Metastatic potential of MDA‐MB‐231 cells has been well demonstrated in vivo, and the same is expected in the case of MDA‐MB‐231 MIG4 subclone.…”

“…In this study we implemented and applied new mass spectrometry tools to identify and verify cell surface proteins that are associated with the higher migration potential of a metastatic breast cancer cell line MDA‐MB‐231 . For this purpose, we selected a more migrating subpopulation of MDA‐MB‐231 cells named MDA‐MB‐231 MIG4.…”

Camouflaged Gold Nanodendrites Enable Synergistic Photodynamic Therapy and NIR Biowindow II Photothermal Therapy and Multimodal Imaging

“…30−32 In addition, the presence of α4 integrin in macrophage membranes allows for active binding to MDA-MB-231 cells, overexpressing vascular cell adhesion molecule (VCAM)-1. 33 As such, coating AuND with macrophage-derived cell membranes would be a viable approach to facilitating the accumulation of AuND in MDA-MB-231 cells.…”

What Is the Role of Imaging in Cancers?

“…Table 1 shows a summary of the topics and the papers included for each topic [ 1 , 2 , 3 , 4 , 5 , 6 , 7 , 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 ]. The majority of papers are focused on prostate cancer (PCa) and radiomics.…”

“…The residual papers focused on different topics by analyzing diverse imaging modalities from preclinical [ 17 ], to gold nanoparticles for CT imaging [ 3 , 4 ], to specific pathologies (i.e., breast cancer [ 1 , 2 ] or pancreas [ 13 , 14 ] or meningioma [ 10 ]), to multicenter trials [ 8 , 22 ]. The common conclusion of these contributions is the current and future support that medical imaging can add to clinical information.…”