In Vivo Assessment of Stem Cells for Treating Neurodegenerative Disease: Current Approaches and Future Prospects

Song, Bomi

doi:10.1155/2017/9751583

Cited by 5 publications

(6 citation statements)

References 39 publications

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“…Recent progress in the field of cell therapies 1 , 2 and the increasing understanding of the complex interplay between different cell populations 3 – 5 have created a demand for novel in vivo methods to longitudinally study the fate of specific cell populations or even individual cells. Optical techniques such as confocal or two-photon microscopy are well established for cell tracking, but require invasive procedures such as installation of cranial windows or skin-fold chambers 6 , 7 .…”

Section: Introductionmentioning

confidence: 99%

Temporal window for detection of inflammatory disease using dynamic cell tracking with time-lapse MRI

Masthoff

Gran²,

Zhang³

et al. 2018

Sci Rep

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Time-lapse MRI was implemented for dynamic non-invasive cell tracking of individual slowly moving intravascular immune cells. Repetitive MRI acquisition enabled dynamic observation of iron oxide nanoparticle (ION) labelled cells. Simulations of MRI contrast indicated that only cells moving slower than 1 µm/s were detectable. Time-lapse MRI of the brain was performed after either IONs or ION-labelled monocytes were injected intravenously into naïve and experimental autoimmune encephalomyelitis (EAE) bearing mice at a presymptomatic or symptomatic stage. EAE mice showed a reduced number of slow moving, i.e. patrolling cells before and after onset of symptoms as compared to naïve controls. This observation is consistent with the notion of altered cell dynamics, i.e. higher velocities of immune cells rolling along the endothelium in the inflamed condition. Thus, time-lapse MRI enables for assessing immune cell dynamics non-invasively in deep tissue and may serve as a tool for detection or monitoring of an inflammatory response.

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Section: Introductionmentioning

confidence: 99%

Temporal window for detection of inflammatory disease using dynamic cell tracking with time-lapse MRI

Masthoff

Gran²,

Zhang³

et al. 2018

Sci Rep

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“…Near-infrared light (NIR) is another noninvasive imaging tool with ability of penetrating deep into tissues. To define the precise location of implanted hMSCs, NIR scanner was used to provide 3D images contain the contour, reconstruction and coronal section at cell-engrafted site of hMSCs [76].…”

Section: Nanotechnology Application For Monitoring Stem-cell Therapy mentioning

confidence: 99%

Application of Nanotechnology in Stem-Cell-Based Therapy of Neurodegenerative Diseases

et al. 2020

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In addition to adverse health outcomes, neurological disorders have serious societal and economic impacts on patients, their family and society as a whole. There is no definite treatment for these disorders, and current available drugs only slow down the progression of the disease. In recent years, application of stem cells has been widely advanced due to their potential of self-renewal and differentiation to different cell types which make them suitable candidates for cell therapy. In particular, this approach offers great opportunities for the treatment of neurodegenerative disorders. However, some major issues related to stem-cell therapy, including their tumorigenicity, viability, safety, metastases, uncontrolled differentiation and possible immune response have limited their application in clinical scales. To address these challenges, a combination of stem-cell therapy with nanotechnology can be a solution. Nanotechnology has the potential of improvement of stem-cell therapy by providing ideal substrates for large scale proliferation of stem cells. Application of nanomaterial in stem-cell culture will be also beneficial to modulation of stem-cell differentiation using nanomedicines. Nanodelivery of functional compounds can enhance the efficiency of neuron therapy by stem cells and development of nanobased techniques for real-time, accurate and long-lasting imaging of stem-cell cycle processes. However, these novel techniques need to be investigated to optimize their efficiency in treatment of neurologic diseases.

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“…Sodium thiosulfate pentahydrate (Proteomics grade, 99%) was purchased from Amresco, LLC (Solon, OH). 1,1,1,3,3, was purchased from Oakwood Products, Inc. (Estill, SC). Sodium sulfate anhydrous (ACS grade) and methanol (ACS grade), hydrochloric acid (36.5-38%, ACS Grade) were purchased from VWR International (Radnor, PA).…”

Section: Materials Used For Nanofiber Synthesis and Cell Studymentioning

confidence: 99%

“…With the potential to differentiate into a wide variety of lineages, ESCs can be used for tissue culture models of diseases or for therapeutic approaches for neurodegenerative diseases. 1,2 Soluble bioactive agents combined with a protein-coated surface are the typical strategy to support and direct differentiation. While use of adsorbed protein is convenient, this method suffers from batch-to-batch variation and the presentation of bioactive protein motifs is uncontrolled and highly variable.…”

Section: ■ Introductionmentioning

confidence: 99%

“…Embryonic stem cells (ESCs) have unique properties of self-renewal and differentiation, making them suitable choices for in vitro models to study various pathologies. With the potential to differentiate into a wide variety of lineages, ESCs can be used for tissue culture models of diseases or for therapeutic approaches for neurodegenerative diseases. , Soluble bioactive agents combined with a protein-coated surface are the typical strategy to support and direct differentiation. While use of adsorbed protein is convenient, this method suffers from batch-to-batch variation and the presentation of bioactive protein motifs is uncontrolled and highly variable.…”

Section: Introductionmentioning

confidence: 99%

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RGD-Functionalized Nanofibers Increase Early GFAP Expression during Neural Differentiation of Mouse Embryonic Stem Cells

et al. 2019

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Stem cell differentiation towards a specific lineage is controlled by its microenvironment. Polymer scaffolds have long been investigated to provide microenvironment cues; however synthetic polymers lack the specific signaling motifs necessary to direct cellular responses on their own. In this study, we fabricated random and aligned poly(ε-caprolactone) nanofiber substrates, surfacefunctionalized with RGD via strain-promoted azide-alkyne cycloaddition, that were used to investigate the role of a covalently tethered bioactive peptide (RGD) and nanofiber orientation on neural differentiation of mouse embryonic stem cells. Gene and protein expression showed neural differentiation progression over 14 days, with similar expression on RGD random and aligned nanofibers for neurons and glia over time. The high levels of glial fibrillary acidic protein expression at early time points were indicative of neural progenitors, and occurred earlier than on controls or in previous reports. These results highlight the influence of RGD binding versus topography in differentiation.

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In Vivo Assessment of Stem Cells for Treating Neurodegenerative Disease: Current Approaches and Future Prospects

Cited by 5 publications

References 39 publications

Temporal window for detection of inflammatory disease using dynamic cell tracking with time-lapse MRI

Temporal window for detection of inflammatory disease using dynamic cell tracking with time-lapse MRI

Application of Nanotechnology in Stem-Cell-Based Therapy of Neurodegenerative Diseases

RGD-Functionalized Nanofibers Increase Early GFAP Expression during Neural Differentiation of Mouse Embryonic Stem Cells

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