In Vivo Application of Optogenetics for Neural Circuit Analysis

Han, Xue

doi:10.1021/cn300065j

Cited by 82 publications

(75 citation statements)

References 68 publications

(124 reference statements)

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“…NpHR) and light-driven proton pumps (e.g. AR3) have been used as inhibitory optogenetics tools in the field of neuroscience [65][66][67]. Recently, Yawo's group and co-workers successfully expressed KR2 in rat cortical neurons, and demonstrated that the KR2-mediated outward Na þ current also inhibits neuronal activation [33].…”

Section: Future Perspectivesmentioning

confidence: 99%

The light‐driven sodium ion pump: A new player in rhodopsin research

Kato

Inoue

Kandori³

et al. 2016

BioEssays

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Rhodopsins are one of the most studied photoreceptor protein families, and ion-translocating rhodopsins, both pumps and channels, have recently attracted broad attention because of the development of optogenetics. Recently, a new functional class of ion-pumping rhodopsins, an outward Na þ pump, was discovered, and following structural and functional studies enable us to compare three functionally different ion-pumping rhodopsins: outward proton pump, inward Cl À pump, and outward Na þ pump. Here, we review the current knowledge on structure-function relationships in these three light-driven pumps, mainly focusing on Na þ pumps. A structural and functional comparison reveals both unique and conserved features of these ion pumps, and enhances our understanding about how the structurally similar microbial rhodopsins acquired such diverse functions. We also discuss some unresolved questions and future perspectives in research of ion-pumping rhodopsins, including optogenetics application and engineering of novel rhodopsins.

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Section: Future Perspectivesmentioning

confidence: 99%

The light‐driven sodium ion pump: A new player in rhodopsin research

Kato

Inoue

Kandori³

et al. 2016

BioEssays

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“…Based on these findings, it is proposed that the LPAG/VLPAG -NA monosynaptic pathway transmits fear-driven central signals, which elicit bradycardia through parasympathetic outflow (Koba et al, 2016). Recently, optogenetic approaches have been used to study the role of basic synaptic properties of specific neural circuits in physiology and behaviour (Britt et al, 2012;Han, 2012).…”

Section: Introductionmentioning

confidence: 99%

“…Channelrhodopsin-2 (ChR2), an algal protein from Chlamydomonas reinhardtii, is the most common light-sensitive protein used (Britt et al, 2012;Han, 2012). Assareh et al…”

Section: Introductionmentioning

confidence: 99%

Genotype-dependent responsivity to inflammatory pain: A role for TRPV1 in the periaqueductal grey

Madasu

Okine

Olango

et al. 2016

Pharmacological Research

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“…Also, when the recording electrodes are illuminated, they generate large artifacts through the Becquerel effect [38,39], complicating data evaluation. Therefore, the process of CGH generation needs to be calibrated.…”

Section: Calibration Of the Illumination Systemmentioning

confidence: 99%

Optogenetic Stimulation of Human Neural Networks Using Fast Ferroelectric Spatial Light Modulator—Based Holographic Illumination

et al. 2018

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Abstract:The generation and application of human stem-cell-derived functional neural circuits promises novel insights into neurodegenerative diseases. These networks are often studied using stem-cell derived random neural networks in vitro, with electrical stimulation and recording using multielectrode arrays. However, the impulse response function of networks is best obtained with spatiotemporally well-defined stimuli, which electrical stimulation does not provide. Optogenetics allows for the functional control of genetically altered cells with light stimuli at high spatiotemporal resolution. Current optogenetic investigations of neural networks are often conducted using full field illumination, potentially masking important functional information. This can be avoided using holographically shaped illumination. In this article, we present a digital holographic illumination setup with a spatial resolution of about 8 µm, which suffices for the stimulation of single neurons, and offers a temporal resolution of less than 0.6 ms. With this setup, we present preliminary single-cell stimulation recording of stem-cell derived induced human neurons in a random neural network. This will offer the opportunity for further studies on connectivity in such networks.

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In Vivo Application of Optogenetics for Neural Circuit Analysis

Cited by 82 publications

References 68 publications

The light‐driven sodium ion pump: A new player in rhodopsin research

The light‐driven sodium ion pump: A new player in rhodopsin research

Genotype-dependent responsivity to inflammatory pain: A role for TRPV1 in the periaqueductal grey

Optogenetic Stimulation of Human Neural Networks Using Fast Ferroelectric Spatial Light Modulator—Based Holographic Illumination

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