In vivo Antioxidant Potential of Raphanus sativus
Seeds in Rat Kidney Against CCl4-Induced Toxicity

Shehzadi, Irum; Shah, Naseer Ali; Khan, Muhammad Rashid; Shuaib, Muhammad; Shah, Muzammil; Khan, Aimal; Ali, Hani S. H. Mohammed; Anwar, Yasir; Hussain, Firasat; Khan, Hayat Muhammad; Khan, Imtiaz; Kaleem, Imdad; Shabbir, Asghar; Riaz, Naveeda; Ul-Haq, Asad; Rasheed, Yaseer; Khan, M. A.; Sher, A.I; Ali, Ijaz; Shaheen, Ghazala

doi:10.15244/pjoes/94453

Cited by 3 publications

(2 citation statements)

References 27 publications

(30 reference statements)

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“…Antioxidant activities of R. sativus was previously demonstrated (Barillari et al, 2006;Beevi et al, 2012;Eveline & Pasau, 2019;Goyeneche et al, 2015;Kim et al, 2016;Noman et al, 2021;Shehzadi et al, 2020;Sonam, 2019). With an RSa50 value of 165.49 µg/mL, the methanol extract of R. sativus showed a better free-radical scavenging activity against DPPH than the 70% ethanol extract which presented an RSa50 value of 359.7 µg/mL in a previous study (Noman et al, 2021), but with a higher total phenol content of 95.8 GAE (compared to 6.17 GAE found in this study).…”

Section: Discussionmentioning

confidence: 91%

Radical Scavenging Activities, Total Reducing Power, Total Phenolic and Flavonoids Contents of Four Common Vegetables

Djeussi

Kenfack

Anangmo

et al. 2022

EJBIO

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Aim: This study aimed at evaluating the antioxidant activities of extracts from four vegetables namely: Beta vulgaris, Raphanus sativus, and two varieties (red and green) of Brassica oleracea. Method: The antioxidant properties of these extracts were assessed using five different methods including 2,2-diphényl 1-picrylhydrazyl (DPPH), Nitrogen Oxyde (NO), total reducing power, total phenolic and flavonoids content. Results: All the four vegetable extracts showed free radical-scavenging activity against DPPH· with RSa50 (Radical scavenging activity 50) ranging between 129.77 and 323.64 µg/ml, and inhibitory activity against NO radical (RSa50 ranging from 1454,52 to 4479,97 µg/ml). The four vegetable extracts also showed total reducing powers ranging between 2.41 and 9.37 AAE (mg ascorbic acid equivalents per gram of dried extract). These antioxidant activities can be justified by the presence of different antioxidant compounds like total phenol contents which were present in all studied vegetable extracts with quantities varying between 4.37 and 11.83 GAE (mg of garlic acid equivalents per gram of dried extract) of dry extract, or flavonoids which were also present in all the plants with total contents ranging between 0.1 and 0.25 RE (rutin equivalents per gram of dried extract). Conclusion: The different antioxidant activities demonstrated in this study provide scientific evidence that some vegetables commonly consumed in Cameroon including B. oleracea, R. sativus and B. vulgaris can serve as a dietary supplement or in preventive medicine in the management of oxidative stress and associated pathologies.

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Section: Discussionmentioning

confidence: 91%

Radical Scavenging Activities, Total Reducing Power, Total Phenolic and Flavonoids Contents of Four Common Vegetables

Djeussi

Kenfack

Anangmo

et al. 2022

EJBIO

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“…This plant is usually consumed as raw food (salad) in Indonesia as a cheap and common source of proteins, vitamins and minerals. Consumption of this plant, especially as fresh food (raw), is believed to be effective in overcoming various health problem such as hepatotoxicity [6], nephrotoxicity [7], cardiotoxicity [8,9], asthma [10], brain dysfunction [11], adrenal dysfunction [12], and oxidative stress [13].…”

Section: Background and Aimsmentioning

confidence: 99%

Evaluation of the anti-gout effect of Sonchus Arvensis on monosodium urate crystal-induced gout arthritis via anti-inflammatory action - an in vivo study

Parisa

Hidayat

Maritska

et al. 2021

Medicine and Pharmacy Reports

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Background and aims. Sonchus arvensis is an Indonesian plant with strong therapeutic effects. Various studies have shown that this plant is useful in treating kidney stone disorders, and recent studies have shown that S. arvensis extract can reduce inflammation caused by monosodium urate crystal deposition in the synovial tissue . This study was aimed to explore the extract of Sonchus arvensis, via fractionation, to optimize the specific content of S. arvensis with anti-inflammatory potential in gout arthritis. Methods. The study included 30 rats (Rattus norvegicus) Wistar strain obtained from the Eureka Research Laboratory (Palembang, Indonesia) weighing between 200 - 250 grams. After one week of acclimatization, the rats were randomly divided into six groups, each group containing five animals; normal control group, monosodium urate group (negative control), colchicine group, hexane fraction of S. arvensis group, ethyl-acetate fraction of S. arvensis group and water fraction group. Before monosodium urate administration, rats in the colchicine group, as a positive control group, were given orally for seven days with 0.28 mg/kg/day colchicine. IL-1β levels in joint synovial fluid were examined with Rat ELISA interleukin-1β. Results. S. arvensis water fraction showed the most significant reduction in inflammatory cells compared to the hexane or ethyl acetate fractions. The water fraction of S. arvensis group had an equal effect with positive control in reducing the infiltration of inflammatory cells in the synovial tissue. Conclusion. Sonchus arvensis water fraction has anti-gout effects in monosodium urate-induced gout arthritis in rats by decreasing the inflammatory response in the synovial joint.

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Toxicity of carbon tetrachloride, free radicals and role of antioxidants

Ünsal

Çiçek

Sabancılar

2020

Reviews on Environmental Health

138

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Several chemicals, including environmental toxicants and clinically useful drugs, cause severe cellular damage to different organs of our body through metabolic activation to highly reactive substances such as free radicals. Carbon tetrachloride is an organic compound of which chemical formula is CCl₄. CCl4 is strong toxic in the kidney, testicle, brain, heart, lung, other tissues, and particularly in the liver. CCl4 is a powerful hepatoxic, nephrotoxic and prooxidant agent which is widely used to induce hepatotoxicity in experimental animals and to create hepatocellular carcinoma, hepatic fibrosis/cirrhosis and liver injury, chemical hepatitis model, renal failure model, and nephrotoxicity model in recent years. The damage-causing mechanism of CCl4 in tissues can be explained as oxidative damage caused by lipid peroxidation which starts after the conversion of CCl4 to free radicals of highly toxic trichloromethyl radicals (•CCl₃) and trichloromethyl peroxyl radical (•CCl₃O2) via cytochrome P450 enzyme. Complete disruption of lipids (i.e., peroxidation) is the hallmark of oxidative damage. Free radicals are structures that contain one or more unpaired electrons in atomic or molecular orbitals. These toxic free radicals induce a chain reaction and lipid peroxidation in membrane-like structures rich in phospholipids, such as mitochondria and endoplasmic reticulum. CCl4-induced lipid peroxidation is the cause of oxidative stress, mitochondrial stress, endoplasmic reticulum stress. Free radicals trigger many biological processes, such as apoptosis, necrosis, ferroptosis and autophagy. Recent researches state that the way to reduce or eliminate these CCl4-induced negative effects is the antioxidants originated from natural sources. For normal physiological function, there must be a balance between free radicals and antioxidants. If this balance is in favor of free radicals, various pathological conditions occur. Free radicals play a role in various pathological conditions including Pulmonary disease, ischemia / reperfusion rheumatological diseases, autoimmune disorders, cardiovascular diseases, cancer, kidney diseases, hypertension, eye diseases, neurological disorders, diabetes and aging. Free radicals are antagonized by antioxidants and quenched. Antioxidants do not only remove free radicals, but they also have anti-inflammatory, anti-allergic, antithrombotic, antiviral, and anti-carcinogenic activities. Antioxidants contain high phenol compounds and antioxidants have relatively low side effects compared to synthetic drugs. The antioxidants investigated in CCI4 toxicity are usually antioxidants from plants and are promising because of their rich resources and low side effects. Data were investigated using PubMed, EBSCO, Embase, Web of Science, DOAJ, Scopus and Google Scholar, Carbon tetrachloride, carbon tetrachloride-induced toxicity, oxidative stress, and free radical keywords. This study aims to enlighten the damage-causing mechanism created by free radicals which are produced by CCl4 on tissues/cells and to discuss the role of antioxidants in the prevention of tissue/cell damage. In the future, Antioxidants can be used as a therapeutic strategy to strengthen effective treatment against substances with high toxicity such as CCl4 and increase the antioxidant capacity of cells.

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In vivo Antioxidant Potential of Raphanus sativus Seeds in Rat Kidney Against CCl4-Induced Toxicity

Cited by 3 publications

References 27 publications

Radical Scavenging Activities, Total Reducing Power, Total Phenolic and Flavonoids Contents of Four Common Vegetables

Radical Scavenging Activities, Total Reducing Power, Total Phenolic and Flavonoids Contents of Four Common Vegetables

Evaluation of the anti-gout effect of Sonchus Arvensis on monosodium urate crystal-induced gout arthritis via anti-inflammatory action - an in vivo study

Toxicity of carbon tetrachloride, free radicals and role of antioxidants

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