In vivo anti-salmonella activity of aqueous extract of Euphorbia prostrata Aiton (Euphorbiaceae) and its toxicological evaluation

Tala, Donald Sédric; Gatsing, Donatien; Fodouop, Siméon Pierre Chegaing; Fokunang, Charles; Kengni, Fabrice; Djimeli, Merline Namekong

doi:10.1016/s2221-1691(15)30350-6

Cited by 22 publications

(24 citation statements)

References 23 publications

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“…Alternatively, a 3-day regime of I/P cyclophosphamide can be used as an immunosuppressive agent before oral infection. 42 The pathologies induced by the infection of S. Enteritidis are mainly inflammatory, with the inflammatory findings in liver similar to those in previous studies on mice, 42,68 including congestion, edema, and inflammatory cells infiltrations. The necrotic changes in intestine are in line with Lee et al 69 The findings of this current study showed that in the groups treated with AgNPs, the severity of inflammatory response is greatly reduced.…”

Section: Dovepresssupporting

confidence: 79%

“…After the acclimatization period, mice were immunosuppressed by intraperitoneal injection with a dose of 30 mg/ kg/24 hours of cyclophosphamide (Endoxan®) for 3 successive days. 42 On the last day of immunosuppression, all mice were fasted overnight followed by oral administration of 0.1 mL of 5% solution of sodium bicarbonate (NaHCO 3 ) to counteract the stomach pH immediately before oral infection. 43 Infection with 1 mL of normal saline solution containing 1.5×10 8 CFU/mL of the isolated Salmonella Enteritidis strain (isolate No.1) given by oral gavage to all infected groups; the other non-infected groups received normal saline only to exclude the stress factor.…”

Section: In Vivo Antimicrobial Activity Assay Experimental Designmentioning

confidence: 99%

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The Role of Silver Nanoparticles in a Treatment Approach for Multidrug-Resistant Salmonella Species Isolates

Farouk

El-Molla

Salib

et al. 2020

IJN

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The main objective of this study is to investigate the antibacterial activity of silver nanoparticles (AgNPs) against multidrug-resistant Salmonella isolates recovered from diarrheic sheep and goats Methods: This study used chemical reduction synthesis of AgNPs to evaluate their antimicrobial effects by estimation of minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) for each isolate using the microplate dilution method and tetrazolium salt reduction test to detect the viability percentage. In vivo treatment efficacy was assessed in mice by determining the viable count of Salmonella Enteritidis recovered from feces and by hematologic, biochemical and histopathologic examinations to confirm that use of AgNPs has no toxic or pathologic effects and to evaluate its ability in tissue regeneration following treatment. Results: All recovered strains were identified as MDR with a prevalence of 4% and 3.6% in sheep and goats, respectively. The results of TEM, DLS, Zeta potential, and FTIR revealed typical characteristics of the synthesized AgNPs. Silver nanoparticles showed antibacterial activity against all recovered strains with MIC of ≤0.02-0.313 μg/mL (mean average 0.085 ±0.126 μg/mL) and MBC of 0.078-1.250 μg/mL (average 0.508±0.315 μg/mL). In vivo efficacy of AgNPs was observed by a reduction in the number of viable S. Enteritidis recovered from feces in an S. Enteritidis infected mouse model, with complete shedding stopping between treatment days 4 and 6. Hematologic, serum biochemical, and histopathologic analyses proved the ability of AgNPs to suppress inflammatory reaction caused by S. Enteritidis infection. Conclusion: The study proved the effective ability of AgNPs to fight MDR Salmonella spp. in vitro and in vivo without adverse effects.

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Section: Dovepresssupporting

confidence: 79%

Section: In Vivo Antimicrobial Activity Assay Experimental Designmentioning

confidence: 99%

The Role of Silver Nanoparticles in a Treatment Approach for Multidrug-Resistant Salmonella Species Isolates

Farouk

El-Molla

Salib

et al. 2020

IJN

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“…(40058/HNC)/AmaryllidaceaePneumonia, ovarian problems, hernia, wounds, dysentery, microbial infection, aphrodisiac, snake bite [24]Leaves, bulbsHippadine, pratorimine, β- d -glucopyranoside sitosterol [25]Antibacterial [25] Euphorbia prostrata Ait. (33585/HNC)/EuphorbiaceaeInfertility, menstrual pain, dysentery, typhoid fever [12]Leaves, whole plantProstratins A, B and C, rugosins A, D, E and G, quercetin 3- O -β-sambubioside, euphorbins G and H, tellimagradin I and II, kaempferol, cosmosiin, rhamnetin-3-galactoside, quercetin, β-amyrine acetate, β-sitosterol, campesterol, stigmasterol and cholesterol [26, 27]Antibacterial, antifungal, bleeding haemorrhoids [12, 28, 29] Markhamia tomentosa K. Schum. (1974/SRF-Cam)/BignoniaceaeEdema, cancer, gout, scrotal elephantiasis, pulmonary troubles, general body pain [30]Leavesβ-sitosterol, β-sitosterol-3- O -β- d -glucopyranoside, dehydroiso-α-lapachone, 2-acetylnaphtho[2,3-b]furan-4,9-dione, pomolic acid, 2-acetyl-6-methoxynaphtho[2,3-b]furan-4,9-dione, β-lapachone, tormentic acid, oleanolic acid, palustrine [31, 32]Antiprotozoal, antihelmintic, antibacterial, antifungal, antiviral, analgesic, anti-inflammatory antioxidant, anti-tumor [31–33] Viscum album L. (2974/HNC)/LoranthaceaeAtherosclerosis, hypertension, cancer, headache, dizziness, palpitation [34, 35]LeavesConiferin, syringin, eleutheroside E, syringaresinol- O -glucoside, ligalbumosides A–E, alangilignoside C, kalopanaxin D, β-amyrin acetate, β-amyrin, lupeol, lupeol acetate oleanolic acid, betulinic acid, stigmasterol, β-sitosterol, trans -α-bergamotene, trans -β-farnesene, loliolide, vomifoliol, 5,7-dimethoxy-flavanone-4′- O -glucoside, 2′-hydroxy-4′,6′-dimethoxychalcone-4- O -glucoside, 5,7-dimethoxyflavanone-4′- O -[2′′- O -(5′′′- O - trans -cinnamoyl)-apiosyl]-glucoside), 2′-hydroxy-4′,6′-dimethoxychalcone-4- O -[2′′- O -(5′′′- O - trans -cinnamoyl)-apiosyl]-glucoside, 2′-hydroxy-3,4′,6′-trimethoxychalcone-4- O -glucoside, 2′-hydroxy-4′,6′-dimethoxychalcone-4- O -[apiosyl(1 → 2)]-glucoside, 5,7-dimethoxyflavanone-4′- O -[apiosyl-(1 → 2)]-glucoside, (2 S )-3′,5,7-trimethoxyflavanone-4′- O -glucoside, 4′- O -[β-apiosyl(1 → 2)]-β-glucosyl]-5-hydroxy-7- O -sinapylflavanone, 3-(4-acetoxy-3,5-dimethoxy)-phenyl-2 E -propenyl-β-glucoside, 3-(4-hydroxy-3,5-dimethoxy)-phenyl-2 E -propenyl-β-glucoside, 4′,5-dimethoxy-7-hydroxy flavanone, 5,7-dimethoxy-4′-hydroxy flavanone [36]Antihypertensive, cytotoxicity, vascular effects, antioxidant, antibacterial [34, 35, 37…”

Section: Methodsmentioning

confidence: 99%

Multidrug resistant bacteria are sensitive to Euphorbia prostrata and six others Cameroonian medicinal plants extracts

2017

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BackgroundMultidrug resistant (MDR) bacteria are responsible for therapeutic failure and there is an urgent need for novels compounds efficient on them.MethodsEleven methanol extracts from seven Cameroonian medicinal plants were tested for their antibacterial activity using broth micro-dilution method against 36 MDR bacterial strains including Escherichia coli, Enterobacter aerogenes, Enterobacter cloacae, Klebsiella pneumoniae, Providencia stuartii, Pseudomonas aeruginosa and Staphylococcus aureus.Results Euphorbia prostrata extract was found active against all the 36 tested bacteria including Gram-negative phenotypes over-expressing efflux pumps such as P. aeruginosa PA124, E. aerogenes CM64 and E. coli AG102. E. prostrata had minimal inhibitory concentrations values between 128 and 256 µg/mL on 55.55% of the studied microorganisms. Other plants extract displayed selective antibacterial activity.ConclusionsResults obtained in this study highlight the antibacterial potential of the tested plants and the possible use of E. prostrata to combat bacterial infections including MDR phenotypes.

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“…Hepatic necrosis and oxidation have always been assessed through the measurement of markers like ASAT and ALAT (Tala et al, 2015;Olaleye et al, 2014;Bidie et al, 2010). In case of hepatotoxicity, the ALAT and ASAT increased simultaneously in the serum (Tala et al, 2015).…”

Section: Toxicity Testmentioning

confidence: 99%

Chemical composition, antibiotic promotion and in vivo toxicity of Piper nigrum and Syzygium aromaticum essential oil

Ismail¹,

Kemegne²,

Njayou³

et al. 2017

Afr. J. Biochem. Res.

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The antimicrobial activity of spices of human daily diet has been widely demonstrated. Yet, their capacity of promoting antibiotic activity has not been explored adequately. In this work, the chemical composition of essential oils of Piper nigrum and Syzygium aromaticum from Chad were determined and their antimicrobial effect in combination with different antibiotics towards Staphylococcus aureus, Salmonella enteritidis and Salmonella typhi were assessed. Moreover, the in vivo toxicity of the essential oils alone and in combination with antibiotics was performed. Results indicate that the essential oil of P. nigrum was mostly composed of limonene (18.59%), beta-pinene (11.51%), linalool (10.17%), alpha-pinene (9.96%), while for S. aromaticum essential oil, only three compounds represented about 89% of the identified compounds. They were respectively beta caryophyllene (43.63%), eugenol (42.67%) and alpha-humulene (3.73%). Regarding the antimicrobial properties, Syzygium aromaticum essential oil (EO) used alone was more active than P. nigrum EO with a minimum inhibitory concentration (MIC) of 625 ppm towards all the strains. In combination with selected antibiotics, S. aromaticum gave better results with less indifference association, the best being with gentamicin. This association of the different EOs with gentamicin proved not to be acutely toxic to rats after 14 days of observation at doses ten times the MIC. These results indicated that spices used in human diets can be used for the production of hybrid drugs containing antibiotic and natural substances.Key words: Piper nigrum, Syzygium aromaticum, antibiotic promotion, Staphylococcus aureus, Salmonella, toxicity. INTRODUCTIONSpices are highly used in Chadian diet and many research works indicate that some of them have biological activities (Beuchat, 1994;Shan et al., 2007). In the continuous search for natural compounds with antimicrobial properties, a more safety approach is to use substances already belonging to human diet. Bacterial infection is one of the most current causes of illness worldwide (Kuete, 2013) and especially in developing countries (Adwan et al., 2008). According to Havelaar et al. (2015), food borne diarrheal disease agents, particularly non-typhoidal Salmonella enterica, cause about 23000 death worldwide, with a high percentage in Africa. In the same report, Salmonella Typhi was recognized as one of the major cause of food borne diseases. Regarding Staphylococcus aureus, Tong et al. (2015) stated that in the industrialized world, the population incidence of S. aureus bacteremia ranges from 10 to 30 per 100,000 person/year.Black pepper (Piper nigrum) has many medicinal properties among which, antibacterial, antifungal, antiinflamatory and antidiarrhoeal are mostly reported (Noumedem et al., 2013). Clove (Syzygium aromaticum) is an aromatic herb that has many useful purposes. Its essential oil has demonstrated several properties as reported by Noumedem et al. (2013). S. aromaticum is widely cultivated in Indonesia, Sri Lanka, Madagascar,...

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In vivo anti-salmonella activity of aqueous extract of Euphorbia prostrata Aiton (Euphorbiaceae) and its toxicological evaluation

Cited by 22 publications

References 23 publications

<p>The Role of Silver Nanoparticles in a Treatment Approach for Multidrug-Resistant <em>Salmonella</em> Species Isolates</p>

<p>The Role of Silver Nanoparticles in a Treatment Approach for Multidrug-Resistant <em>Salmonella</em> Species Isolates</p>

Multidrug resistant bacteria are sensitive to Euphorbia prostrata and six others Cameroonian medicinal plants extracts

Chemical composition, antibiotic promotion and in vivo toxicity of Piper nigrum and Syzygium aromaticum essential oil

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