In vivo ANTAGONISM OF Fusarium oxysporum f. sp. Phaseoli, AND GROWTH PROMOTION IN COMMON BEAN (Phaseolus vulgaris) (FABACEAE) BY Trichoderma spp.

Abstract

The aim of this study was to evaluate the pathogenic capacity of Fusarium oxysporum, the antagonistic capacity, and growth promotion of the strains of Trichoderma spp. in vivo in common beans. The experiment was carried in two steps. First, the pathogenicity evaluation test of the F. oxysporum strain was carried out in 200 mL pots, and the biometry of the bean plants was subsequently evaluated. In the second experiment, were used pots of 5L containing sterile soil. Four strains of Trichoderma, and one strain of F. oxysporum were used. The bean seeds were previously sterilized, followed by inoculation with the pathogen previously grown in Petri dishes with Mannitol, followed by planting the infected seeds. The antagonists were grown in petri dishes, and after five days scraping was performed obtaining a spore suspension (10-4 spores per ml), followed by direct inoculation in the soil after planting the seeds. In all experiments, a completely randomized design with three replications was adopted. After 57 days, their agronomic and biometric characters were evaluated, namely: number of leaves, plant height, basal diameter, root length, root weight, leaf area. The data obtained were tabulated, and submitted to analysis of variance (ANOVA), and the means compared by the Skott-Knott test (P = .05). Plants treated with Trichoderma inoculum show the best results regarding biometric parameters, can acting as plant growth promoters. Among these parameters, biomass and dry mass are the most significant for this crop. Trichoderma strains used in this study can inhibit the damage of F. oxysporum, and promote bean growth.

Evaluación de Trichoderma en tres variedades de maní (Arachis hypogaea L.)

“…Estos resultados indican que Trichoderma favorece el rendimiento de los cultivos incluso en dosis más bajas (1x10 9 ) según Chagas et al (2019). El efecto antagonista de Trichoderma independiente de la dosis (Tabla 4) y la cepa (Tabla 3) redujo al 15,6 % la incidencia de moho blanco y al 17,8 % la incidencia de roya, mostrando la acción preventiva y el control de fitopatógenos (Campanioni et al 2019;Chagas et al, 2019;Medeiros et al, 2022). Sig.…”