In Vivo Animal Models: Quantitative Models Used for Identifying Antibacterial Agents

Abstract

Determining the in vivo bactericidal potential of novel agents is critical for selecting drug candidates. Described in this unit are two protocols that measure the amount of bacterial killing under very different conditions. The mouse pouch protocol is a rapid assay that provides efficacy data in an acute infection, whereas the rabbit tissue cage protocol assesses the ability of a compound to eradicate bacteria in a chronic abscess infection. Data from these tests, along with those from other qualitative and quantitative assays, are important for defining antibacterial efficacy in vivo.

Effect of erythromycin‐doped calcium polyphosphate scaffold composite in a mouse pouch infection model

“…13,[33][34][35] This model has been used as a valuable alternative to conventional osteomyelitis model, because it preserves the sensitivity and reproducibility of the pouch tissue in response to bacterial infection under controlled experimental conditions. [36][37][38][39] This model allows us to not only measure the bactericidal activity of EM released but also to examine the morphological changes both in the implanted scaffolds and in the pouch tissues induced by the treatment. In addition, the effects of the combined presence of bacteria and bone graft substitutes in this pouch model has not been well investigated.…”