IN VIVO AND IN VITRO RECOMBINATION BETWEEN DNA MOLECULES IN OPTIMIZED CaCl2-DEPENDENT TRANSFECTION SYSTEMS – CLONING OF THE OCR GENE

Pfeifer, Marco; Betcke, A.; Pöhlmann, Ch.; Kurth, Marion; Liebscher, D.-H.

doi:10.1016/b978-0-08-025494-4.50056-5

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General Properties the Viruses And Their Growth Cycle1

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1981

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“…Campbell et al (50) cloned some late and early T7 genes on the plasmid pMB9; in addition, the origin ofDNA replication (51, 356, 423a) and promoter regions were cloned (346,347,435). Recently, the T7 gene 0.3 (ocr+) has been inserted into a bacteriophage lambda vector (363). Tsujimoto and co-workers took advantage of the fact that T7 wild-type DNA lacks EcoRI recognition sites to study its recombination with DNA of an EcoRI-sensitive T7 mutant (341,(508)(509)(510).…”

Section: General Properties the Viruses And Their Growth Cyclementioning

confidence: 99%