In vivo and in vitro evaluation of pharmacological activities of Adenia trilobata (Roxb.)

Barua, Niloy; Aziz, Arfin Ibn; Tareq, Abu Montakim; Sayeed, Mohammed Aktar; Alam, Najmul; Alam, Nobi ul; Uddin, Mohammad Nazim; Lyzu, Chadni; Emran, Talha Bin

doi:10.1016/j.bbrep.2020.100772

Cited by 23 publications

(26 citation statements)

References 41 publications

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“…In this test, pain sensation is perceived by producing pro-inflammatory endogenous mediators (prostaglandins, cytokines, histamine, lipoxygenase, cyclooxygenase and serotonin) in the peripheral tissue which not only influence the stimulation of prostaglandin but also enhance the capillary permeability progresses of inflammatory ache [ 26 ]. Analgesics are the chemical substances that lower the amount of writhing by inhibiting prostaglandin synthesis [ 27 ]. In the acetic acid writhing test, the MEBPL exposed a significant and dose dependent inhibition in the nociceptive nerve.…”

Section: Resultsmentioning

confidence: 99%

Unravelling the Biological Activities of the Byttneria pilosa Leaves Using Experimental and Computational Approaches

et al. 2020

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Byttneria pilosa is locally known as Harijora, and used by the native hill-tract people of Bangladesh for the treatment of rheumatalgia, snake bite, syphilis, fractured bones, elephantiasis and an antidote for poisoning. The present study was carried out to determine the possible anti-inflammatory, analgesic, neuropharmacological and anti-diarrhoeal activity of the methanol extract of B. pilosa leaves (MEBPL) through in vitro, in vivo and in silico approaches. In the anti-inflammatory study, evaluated by membrane stabilizing and protein denaturation methods, MEBPL showed a significant and dose dependent inhibition. The analgesic effect of MEBPL tested by inducing acetic acid and formalin revealed significant inhibition of pain in both tests. During the anxiolytic evaluation, the extract exhibited a significant and dose-dependent reduction of anxiety-like behaviour in mice. Similarly, mice treated with MEBPL demonstrated dose-dependent reduction in locomotion effect in the open field test and increased sedative effect in the thiopental sodium induced sleeping test. MEBPL also showed good anti-diarrheal activity in both castor oil induced diarrheal and intestinal motility tests. Besides, a previously isolated compound (beta-sitosterol) exhibited good binding affinity in docking and drug-likeliness properties in ADME/T studies. Overall, B. pilosa is a biologically active plant and could be a potential source of drug leads, which warrants further advanced study.

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Section: Resultsmentioning

confidence: 99%

Unravelling the Biological Activities of the Byttneria pilosa Leaves Using Experimental and Computational Approaches

et al. 2020

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“…In such cases, medicinal plants should be applied and they give an effective result in pharmacologically and phytochemically [22]. In the current study, we mainly showed that the effects of the methanolic extract of Chukrasia velutina leaves on the central nervous system [6] through multiple numbers of animal models. Depression and anxiety are those mental problems that divert mental health in many pathways such as insomnia, Alzheimer disease, etc.…”

Section: Discussionmentioning

confidence: 66%

“…It creates depression and anxiety in the body by producing a free radical that turns the body into oxidative stress [3]. Moreover, anxiety is also a usual psychiatric problem caused by oxidative stress and affects a large number of people in this world [6]. When anxiety is in the severe stage, it is a huge CNS problem and a massive number of chronic diseases arise from it [7].…”

Section: Introductionmentioning

confidence: 99%

GC-MS Phytochemical Profiling, Pharmacological Properties, and In Silico Studies of Chukrasia velutina Leaves: A Novel Source for Bioactive Agents

et al. 2020

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Chukrasia velutina is a local medicinal plant commonly known as chikrassy in Bangladesh, India, China, and other South Asian countries. The leaves, bark, and seeds are vastly used as herbal medicine for fever and diarrhea, and its leaves essential oils are used for antimicrobial purposes. In this study, we discuss the neuropsychiatric properties of C. velutina leaves through several animal models, quantitative and qualitative phytochemical analysis, and computational approaches. Neuropsychiatric effects were performed in rodents on the methanolic extract of C. velutina leaves (MECVL). Antidepressant, anxiolytic, and sedative effects experimented through these rodent models were used such as the force swimming test (FST), tail suspension test (TST), hole board test (HBT), elevated plus maze test (EPMT), light/dark box test (LDBT), open field test (OFT), and hole cross test (HCT). In these rodent models, 200 and 400 mg/kg doses were used which exhibited a significant result in the force swimming and tail suspension test (p < 0.001) for the antidepressant effect. In the anxiolytic study, the results were significant in the hole board, elevated plus maze, and light/dark box test (p < 0.001) for doses of 200 and 400 mg/kg. The result was also significant in the open field and hole cross test (p < 0.001) for sedative action in the sake of similar doses. Moreover, qualitative and quantitative studies were also performed through phytochemical screening and GC-MS analysis, and fifty-seven phytochemical compounds were found. These compounds were analyzed for pharmacokinetics properties using the SwissADME tool and from them, thirty-five compounds were considered for the molecular docking analysis. These phytoconstituents were docking against the human serotonin receptor, potassium channel receptor, and crystal structure of human beta-receptor, where eight of the compounds showed a good binding affinity towards the respective receptors considered to the reference standard drugs. After all of these analyses, it can be said that the secondary metabolite of C. velutina leaves (MECVL) could be a good source for inhibiting the neuropsychiatric disorders which were found on animal models as well as in computational studies.

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“…Drugs are available on the market that convert plasminogen to plasmin and remove clot lysis [35,36]. Clot lysis is also useful for treating clot disorders, including myocardial infarction, thromboembolic stroke, deep vein thrombosis, and pulmonary embolism [37]. Both MEAM and streptokinase exhibited a moderate degree of clot lysis in comparison with in the control group as depicted by our recent findings.…”

Section: Discussionmentioning

confidence: 77%

Insightful Valorization of the Biological Activities of Pani Heloch Leaves through Experimental and Computer-Aided Mechanisms

et al. 2020

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Pani heloch (Antidesma montanum) is traditionally used to treat innumerable diseases and is a source of wild vegetables for the management of different pathological conditions. The present study explored the qualitative phytochemicals; quantitative phenol and flavonoid contents; in vitro antioxidant, anti-inflammatory, and thrombolytic effects; and in vivo antipyretic and analgesic properties of the methanol extract of A. montanum leaves in different experimental models. The extract exhibited secondary metabolites including alkaloids, flavonoids, flavanols, phytosterols, cholesterols, phenols, terpenoids, glycosides, fixed oils, emodines, coumarins, resins, and tannins. Besides, Pani heloch showed strong antioxidant activity (IC50 = 99.00 µg/mL), while a moderate percentage of clot lysis (31.56%) in human blood and significant anti-inflammatory activity (p < 0.001) was achieved with the standard. Moreover, the analgesic and antipyretic properties appeared to trigger a significant response (p < 0.001) relative to in the control group. Besides, an in silico study of carpusin revealed favorable protein-binding affinities. Furthermore, the absorption, distribution, metabolism, excretion, and toxicity analysis and toxicological properties of all isolated compounds adopted Lipinski’s rule of five for drug-like potential and level of toxicity. Our research unveiled that the methanol extract of A. montanum leaves exhibited secondary metabolites that are a good source for managing inflammation, pyrexia, pain, and cellular toxicity. Computational approaches and further studies are required to identify the possible mechanism which responsible for the biological effects.

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In vivo and in vitro evaluation of pharmacological activities of Adenia trilobata (Roxb.)

Cited by 23 publications

References 41 publications

Unravelling the Biological Activities of the Byttneria pilosa Leaves Using Experimental and Computational Approaches

Unravelling the Biological Activities of the Byttneria pilosa Leaves Using Experimental and Computational Approaches

GC-MS Phytochemical Profiling, Pharmacological Properties, and In Silico Studies of Chukrasia velutina Leaves: A Novel Source for Bioactive Agents

Insightful Valorization of the Biological Activities of Pani Heloch Leaves through Experimental and Computer-Aided Mechanisms

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