In vivo and in vitro characteristics of six Paracoccidioides brasiliensis strains

Abstract

The yeast-like forms of six P. brasiliensis strains were characterized and compared using in vitro (growth curve determination) and in vivo (pathogenicity to sensitive inbred mice) criteria. Strains Pb 18 and Pb 265 which behaved similarly in vitro, showing low counts of fungi and long mean generation times, were respectively the most and the least pathogenic strains. Strains Pb 2052 and IVIC Pb 267, which grow abundantly in vitro were, respectively virulent and avirulent. Strains Pb SN and IVIC Pb 9 behaved similarly both in vitro and in vivo displaying an intermediate pattern of virulence and growing conditions.

Depletion of CD8+T Cells In Vivo Impairs Host Defense of Mice Resistant and Susceptible to Pulmonary Paracoccidioidomycosis

“…P. brasiliensis Pb18, a highly virulent isolate (21), was used throughout this investigation. To ensure the maintenance of its virulence, the isolate was used after three animal passages (22).…”

Alveolar macrophages from susceptible mice are more competent than those of resistant mice to control initialParacoccidioides brasiliensisinfection

“…brasiliensis Pb18, a highly virulent isolate [19], was used throughout this investigation. To ensure the maintenance of its virulence, the isolate was used after three animal passages [8].…”

Evidence for the Role of Calcineurin in Morphogenesis and Calcium Homeostasis during Mycelium-to-Yeast Dimorphism of Paracoccidioides brasiliensis

“…Paracoccidioides brasiliensis isolate 18, which is the prototype of a highly virulent isolate (13), was provided by Z. P. Camargo, UNIFESP, Sao Paulo, Brazil. The fungus was grown at either 25°C or 37°C under rotation at 120 rpm in liquid YPD medium (1% yeast extract, 2% peptone, 2% dextrose) for 5 to 6 days until near the end of the exponential phase.…”