In Vivo and in Vitro Immunomodulatory and Anti-Inflammatory Effects of Total Flavonoids of Astragalus

Guo, Zhendong; Xu, Hongyan; Xu, Lu; Wang, Shasha; Zhang, Xuemei

doi:10.21010/ajtcam.v13i4.10

Cited by 36 publications

(32 citation statements)

References 34 publications

(8 reference statements)

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“…A previous study also reported that LBE is capable of inhibiting NO production by inhibiting NF-κB in vitro [15]. As mentioned previously, genistein, quercetin, daidzein, and naringenin are affluent in LBE, and previous research showed that these compounds inhibited NF-κB activation along with decreased iNOS expression and NO production in vitro [41][42][43] and in vivo [44][45][46]. Therefore, it might be inferred that LBE supplementation might reduce hepatic oxidative stress along with NFκB associated inflammatory responses in T2DM.…”

Section: Discussionmentioning

confidence: 63%

Effects of Lespedeza Bicolor Extract on Regulation of AMPK Associated Hepatic Lipid Metabolism in Type 2 Diabetic Mice

et al. 2019

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Lespedeza bicolor (LB) is one of the ornamental plants used for the treatment of inflammation caused by oxidative damage. However, its beneficial effects on hyperglycemia-induced hepatic damage and the related molecular mechanisms remain unclear. We hypothesized that Lespedeza bicolor extract (LBE) would attenuate hyperglycemia-induced liver injury in type 2 diabetes mellitus (T2DM). Diabetes was induced by a low dosage of streptozotocin (STZ) injection (30 mg/kg) with a high fat diet in male C57BL/6J mice. LBE was administered orally at 100 mg/kg or 250 mg/kg for 12 weeks. LBE supplementation regardless of dosage ameliorated plasma levels of hemoglobin A1c (HbA1c) in diabetic mice. Moreover, both LBE supplementations upregulated AMP-activation kinase (AMPK), which may activate sirtuin1 (SIRT) associated pathway accompanied by decreased lipid synthesis at low dose of LBE supplementation. These changes were in part explained by reduced protein levels of oxidative stress (nuclear factor erythroid 2-related factor 2 (Nrf2) and catalase), inflammation (nuclear factor kappa B (NF-κB), interleukin-1β (IL-1β), interleukin-6 (IL-6), and nitric oxide synthases (iNOS)), and fibrosis (α-smooth muscle actin (α-SMA) and protein kinase C (PKC)) in diabetic liver. Taken together, LBE might be a potential nutraceutical to ameliorate hepatic damage by regulation of AMPK associated pathway via oxidative stress, inflammation, and fibrosis in T2DM.

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Section: Discussionmentioning

confidence: 63%

Effects of Lespedeza Bicolor Extract on Regulation of AMPK Associated Hepatic Lipid Metabolism in Type 2 Diabetic Mice

et al. 2019

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“…Previous studies have showed that FAC has the function of free radical scavenging, resistance to body mutations, the protection of the liver function, anti-myocardial ischemia, reduction of the inflammatory response, and immunity enhancement [ 3 , 13 , 14 ]. The beneficial biological function of FAC makes it possible to apply to clinical disease prevention and treatment.…”

Section: Discussionmentioning

confidence: 99%

Immune Regulation of RAW264.7 Cells In Vitro by Flavonoids from Astragalus complanatus via Activating the NF-κB Signalling Pathway

Ning

Zou

et al. 2018

Journal of Immunology Research

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The current study aimed at investigating the effects of flavonoids from Astragalus complanatus (FAC) on the proliferation, the contents, and gene expression levels of cytokines, secretion of surface stimulating factors, cell cycle, and the expression level of the NF-κB signalling pathway in RAW264.7 cells. Our results revealed that compared with control group, the contents of IL-6, IL-1β, TNF-α, and NO and the mRNA expression levels of IL-6, IL-1β, TNF-α, and iNOS in FAC-treated groups significantly increased (p < 0.01). Moreover, FAC induced macrophage activation to release the above-mentioned mediators partly involved in NF-κB/MAPK signalling pathways. Therefore, FAC regulates immune function in RAW264.7 cells via activating the NF-κB signalling pathway. FAC could be applicable for agriculture, drug research, and food industry as a potent immune-modulatory agent.

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“…Immunomodulatory functions of AR flavonoids were also well reported in vivo [ 60 ]. AR flavonoids could promote lymphocyte proliferation, increase T cell number, modulate T cell subsets disorders, and elevate LAK activity induced by IL-2 in immunosuppressed mice [ 60 , 61 , 62 , 63 ].…”

Section: The Pharmaceutical Value Of Ar Extract and Ar Major Ingrementioning

confidence: 99%

Evaluation of the Pharmaceutical Properties and Value of Astragali Radix

et al. 2018

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Astragali Radix (AR), a Chinese materia medica (CMM) known as Huangqi, is an important medicine prescribed in herbal composite formulae (Fufang) by Traditional Chinese medicine (TCM) practitioners for thousands of years. According to the literature, AR is suggested for patients suffering from “Qi”- and “Blood”-deficiencies, and its clinical effects are reported to be related to anti-cancer cell proliferation, anti-oxidation, relief of complications in cardiovascular diseases, etc. The underlying cell signaling pathways involved in the regulation of these various diseases are presented here to support the mechanisms of action of AR. There are two botanical sources recorded in China Pharmacopoeia (CP, 2015): Astragalus membranaceus (Fisch.) Bge. Var. mongohlicus, (Bge.) Hsiao, and Astragalus membranaceus (Fisch.) Bge. (Fam. Leguminosae), whose extracts of dried roots are processed via homogenization-assisted negative pressure cavitation extraction. Geographic factors and extraction methods have impacts on the pharmaceutical and chemical profiles of AR. Therefore, the levels of the major bioactive constituents of AR, including polysaccharides, saponins, and flavonoids, may not be consistent in different batches of extract, and the pharmaceutical efficacy of these bioactive ingredients may vary depending on the source. Therefore, the present review mainly focuses on the consistency of the available sources of AR and extracts and on the investigation of the biological functions and mechanisms of action of AR and of its major bioactive constituents. Furthermore, it will also include a discussion of the most popular AR composite formulae to further elucidate their chemical and biological profiles and understand the pharmaceutical value of AR.

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In Vivo and in Vitro Immunomodulatory and Anti-Inflammatory Effects of Total Flavonoids of Astragalus

Abstract: Background: Astragali Radix has long been used to improve immune function in traditional Chinese medicine. However, its main active

Cited by 36 publications

References 34 publications

Effects of Lespedeza Bicolor Extract on Regulation of AMPK Associated Hepatic Lipid Metabolism in Type 2 Diabetic Mice

Effects of Lespedeza Bicolor Extract on Regulation of AMPK Associated Hepatic Lipid Metabolism in Type 2 Diabetic Mice

Immune Regulation of RAW264.7 Cells In Vitro by Flavonoids from Astragalus complanatus via Activating the NF-κB Signalling Pathway

Evaluation of the Pharmaceutical Properties and Value of Astragali Radix

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