In Vivo and in Vitro Toxicity of Fractionated Fish Lipids, with Particular Regard to their Content of Chlorinated Organic Compounds

Håkansson, Helen; Sundin, Peter; Andersson, Tommy; Brunström, Björn; Dencker, Lennart; Engwall, Magnus; Ewald, Göran; Gilek, Michael; Holm, Gisela; Honkasalo, Sirpa; Idestam-Almquist, J.; Jonsson, Per R.; Kautsky, Nils; Lundberg, George D.; Lund-Kvernheim, A.; Martinsen, Kari; Norrgren, Leif; Personen, M.; Rundgren, Mats; Stalberg, M.; Tarkpea, Maria; Wesén, Clas

doi:10.1111/j.1600-0773.1991.tb01329.x

Cited by 41 publications

(30 citation statements)

References 27 publications

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“…Fish primary hepatocyte cultures have been used to detect P4501 A1-inducing compounds in organic fractions extracted from pulp and paper mill effluent (Pesonen and Andersson 1992) and in fractions extracted from Baltic salmon (Hakansson et al 1991). Gagne et al(1995) used trout hepatocytes to detect persistent genotoxin compounds in a sediment extract obtained in the vicinity of a creosote-treated wharf, and compared results with chemical analysis for PAHs in the same sediment.…”

Section: Discussionmentioning

confidence: 99%

Optimization of the isolation and cultivation of Cyprinus carpio primary hepatocytes

Yang¹,

He²,

Liu³

et al. 2008

Cytotechnology

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The aquatic environment is affected by numerous chemical contaminants. There is an increasing need to identify these chemicals and to evaluate their potential toxicity towards aquatic life. In this research we optimized techniques for primary cell culture of Cyprinus carpio hepatocytes as one adjunct model for ecotoxicological evaluation of the potential hazards of xenobiotics in the aquatic environment. In this study, Cyprinus carpio hepatocytes were isolated by mechanical separation, two-step collagenase perfusion, and pancreatin digestion. The hepatocytes or parenchymal cells could be separated from cell debris and from non-parenchymal cells by low-speed centrifugation (Percoll gradient centrifugation). The harvested hepatocytes were suspended in DMEM, M199 (cultured in 5% CO 2 ), or L-15 (cultured without 5% CO 2 ) medium then cultured at 17, 27, or 37°C. Cell yield was counted by use of a hemocytometer, and the viability of the cells was assessed by use of the Trypan blue exclusion test. Results from these studies showed that the best method of isolation was pancreatin digestion (the cell yield was 2.7 9 10 8 per g (liver weight) and the viability was 98.4%) and the best medium was M199 (cultured in 5% CO 2 ) or L-15 (cultured without 5% CO 2 ). The optimum culture temperature was 27°C. The primary hepatocytes culture of Cyprimus carpio grew well and satisfied requirements for most toxicological experiments in this condition.

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Section: Discussionmentioning

confidence: 99%

Optimization of the isolation and cultivation of Cyprinus carpio primary hepatocytes

Yang¹,

He²,

Liu³

et al. 2008

Cytotechnology

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“…13 This may explain the reproductive effects reported for fish fed diets high in EOC1. 9 The viability of 'first spawn' cod eggs is known to be lower than in successive spawnings. 14 Perhaps the high EOC1 content of the 'first spawn' eggs observed in this study reduces their viability.…”

Section: Resultsmentioning

confidence: 99%

“…EOC1 from wild fish has been demonstrated to reduce the number and viability of eggs laid by zebrafish as well as other toxicological effects. 9 Although lipids high in EOC1 have been shown to have biological effects, concentrations of EOC1 and EOBr in the lipids from different tissues in fish has previously not been determined. The present work was initiated to determine the distribution of EOC1 and EOBr + E-mail: Kiceniuk@is.dal.ca 0236 5731/98/USD 17.00 9 1998 Akaddmiai Kiad6, Budapest All rights reserved in various tissues, eggs, and bile of individual cod and thereby obtain an indication of the toxicological mechanisms and potential consequences of humans and other animals being exposed to these unidentified compounds.…”

Section: Introduction +mentioning

confidence: 99%

Determination of extractable organic bromine and chlorine in biological compartments of Atlantic cod (Gadus morhua) by neutron activation analysis

Kiceniuk¹,

Zwicker

Chatt

1998

J Radioanal Nucl Chem

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Tissues, eggs and bile of adult Atlantic cod raised in captivity and fed natural food with no additional halogenated compounds of any kind were analyzed by neutron activation. There was a large difference in the tissue distributions of extractable organic chlorine (EOC1) and bromine (EOBr). Liver lipid had the lowest levels of EOBr and EOC1, of all the components investigated, and eggs had the highest levels of EOC1. Heart contained the most EOBr and EOC1 per unit lipid, of the tissues. The presence of a larger proportion of EOBr than EOC1 in bile suggests a higher rate of excretion of EOBr. The EOC1 concentratios found in cod egg lipids were about 5 times higher than the highest values previously reported for EOC1 in lipid.

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“…Six AG isomères, 9-Cl-10-OH-16:0 (9-chloro-10-hydroxypalmitique), 10-Cl-9-OH-16:0, 9-Cl-10-OH-18:0, 10-Cl-9-OH-18:0, 11-Cl-12-OH-18:0 et 12-Cl-11-OH-16:0 y ont été identifiés pour la première fois dans cette classe d'invertébrés (White et Hager, 1977) (figure 11). L'acide 9,10-dichloro-octadécanoïque a été isolé des lipides de A. aurita (White, Hager, 1977) et aussi de ceux de l'anguille Anguilla anguilla (Hakansson et al, 1991). Ce dernier AG, les acides 5,6-dichlorotétradé-canoïque et 7,8-dichlorohexadécanoïque étaient présents sous les formes threo et erythro dans les lipides de plusieurs espèces de poissons comme l'anguille, le saumon, la perche, le hareng de la Baltique et de la mer du Nord (Mu et al, 1996(Mu et al, , 1997.…”

Section: Ag Chlorésunclassified

Acides gras inhabituels des organismes marins : une illustration de la biodiversité moléculaire marine

Barnathan

2010

OCL

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In addition to the common polyunsaturated fatty acids (FA) such as those of the n-3 and n-6 series with major interest in health and nutrition, marine organisms (invertebrates and algae) produce unique FA including branched, methoxylated, halogenated, acetylenic and non-methylene-interrupted structures. The biosynthetic pathways, and the structural and functional roles of these uncommon FA are often not yet known. This short overview wishes to be an outline on the particular FA classes possessing uncommon structures, especially those of increasing interest regarding their promising biological activities.

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In Vivo and in Vitro Toxicity of Fractionated Fish Lipids, with Particular Regard to their Content of Chlorinated Organic Compounds

Cited by 41 publications

References 27 publications

Optimization of the isolation and cultivation of Cyprinus carpio primary hepatocytes

Optimization of the isolation and cultivation of Cyprinus carpio primary hepatocytes

Determination of extractable organic bromine and chlorine in biological compartments of Atlantic cod (Gadus morhua) by neutron activation analysis

Acides gras inhabituels des organismes marins : une illustration de la biodiversité moléculaire marine

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