In Vivo and in Vitro SPRR1 Gene Expression in Normal and Malignant Keratinocytes

Yaar, Mina; Eller, Mark S.; Bhawan, Jag; Harkness, Daniel D; DiBenedetto, P.; Gilchrest, Barbara A.

doi:10.1006/excr.1995.1081

Cited by 39 publications

(25 citation statements)

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“…Therefore, we speculate that expression of SPRRs is an essential feature of SCD of distal bronchiolar cells and plays a very important role in providing a physical and chemical barrier against the environment. In support of this notion, a selective inducible expression of SPRRs, but not involucrin, was noticed in malignant keratinocytes that were induced to differentiate (32), underscoring an important role for SPRRs in epithelial cell differentiation. Moreover, Chinese hamster ovary cells, a nondifferentiating cell type, express SPRR1 at specific stages of the cell cycle indicating that SPRRs may play a novel role in cell cycle progression and growth arrest (26).…”

Section: Discussionmentioning

confidence: 66%

JNK1 and AP-1 regulate PMA-inducible squamous differentiation marker expression in Clara-like H441 cells

Vuong

Patterson

Adiseshaiah

et al. 2002

American Journal of Physiology-Lung Cellular and Molecular Phys

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Exposure of distal bronchiolar region to various toxicants and pollutants suppresses Clara cell differentiation marker expression and greatly enhances the induction of squamous cell differentiation (SCD). Here, we demonstrate for the first time phorbol 13-myristate 12-acetate (PMA)-inducible expression of SCD markers, SPRRs, in Clara-like H441 cells. The transcriptional stimulation of human SPRR1B expression is mainly mediated by a −150- to −84-bp region that harbors two critical activator protein (AP)-1 sites. In unstimulated cells, the −150- to −84-bp region is weakly bound by AP-1 proteins, mainly JunD and Fra1. However, PMA prominently induced the binding of JunB and Fra1. Consistent with this, overexpression of wild-type Jun proteins upregulated the SPRR1B promoter activity. Conversely, a c- jun mutant suppressed both basal and PMA-inducible reporter gene expression. Intriguingly, overexpression of fra2 suppressed PMA-inducible reporter activity, whereas fra1 significantly enhanced basal level activity, indicating an opposing role for these proteins in SPRR1B expression in a manner similar to that observed in proximal tracheobronchial epithelial cells (BEAS-2B clone S6). Interestingly, unlike in S6 cells, a catalytically inactive c-Jun NH2-terminal kinase (JNK) 1 mutant significantly reduced the PMA-inducible SPRR1B promoter activity in H441 cells. Thus either temporal expression and/or spatial activation of AP-1 proteins by JNK1 might contribute to the induction of SCD in Clara cells.

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Section: Discussionmentioning

confidence: 66%

JNK1 and AP-1 regulate PMA-inducible squamous differentiation marker expression in Clara-like H441 cells

Vuong

Patterson

Adiseshaiah

et al. 2002

American Journal of Physiology-Lung Cellular and Molecular Phys

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“…For example, SPR1a (cornifin α) and certain SPR2 proteins are expressed in dry epithelia such as the epidermis; distinctly different members of the SPR2 class are expressed in internal epithelia; and SPR3 is abundantly expressed in mucosal epithelia exposed to mechanical stress, such as the esophagus and rodent forestomach, while it is absent in the epidermis (Fujimoto et al, 1993;Hohl et al, 1995;Steinert et al, 1998b;Song et al, 1999). However, most members are induced in response to UV damage and phorbol esters (Kartasova and van de Putte, 1988;Kartasova et al, 1988b;Gibbs et al, 1990) or malignancy (Yaar et al, 1995). SPR1 and 2 transcription is induced by Ca 2 + through a complex array of interacting AP1, Sp1, ets and other transcription factors (Fischer et a l., 1996;Sak et al, 1998).…”

Section: Structural Protein Components Of Cesmentioning

confidence: 99%

Bricks and mortar of the epidermal barrier

Nemes

Steinert²

1999

Exp Mol Med

502

414

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“…Retinoic acid downregulates the expression of SPRR2 and 3 transcripts (26). Expression of SPRR1 protein or mRNA is induced by calcium, interleukin-1 and 3, but not by increasing cell density (24, 96,102). The effects of phorbol esters depends on JUN and TRE motifs (103,104).…”

Section: Components Of Cesmentioning

confidence: 99%

Structural organization of cornified cell envelopes and alterations in inherited skin disorders

Ishida‐Yamamoto

Iizuka

1998

Experimental Dermatology

107

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The cornified cell envelope is a highly insoluble and extremely tough structure formed beneath the cell membrane during terminal differentiation of keratinocytes. Its main function is to provide human skin with a protective barrier against the environment. Sequential crosslinking of several integral components catalyzed by transglutaminases leads to a gradual increase in the thickness of the envelope and underscores its rigidity. Key structural players in this cross-linking process include involucrin, loricrin, SPRRs, elafin, cystatin A, S100 family proteins, and

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In Vivo and in Vitro SPRR1 Gene Expression in Normal and Malignant Keratinocytes

Cited by 39 publications

References 0 publications

JNK1 and AP-1 regulate PMA-inducible squamous differentiation marker expression in Clara-like H441 cells

JNK1 and AP-1 regulate PMA-inducible squamous differentiation marker expression in Clara-like H441 cells

Bricks and mortar of the epidermal barrier

Structural organization of cornified cell envelopes and alterations in inherited skin disorders

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