In vivo and in vitro anti-obesity activities of dihydropyranocoumarins derivatives from Peucedanum japonicum Thunb

Dihydropyranocoumarins Exerted Anti-Obesity Activity In Vivo and its Activity Was Enhanced by Nanoparticulation with Polylactic-Co-Glycolic Acid

“…In this study, we investigated the effects of DPCs, such as PCIII and PTX isolated from PJT, on the development of obesity in HFD-fed mice. Although the results of our previous study did not remove the possibility that other ingredients in DPC concentrate (purity~60%) have anti-obesity effects [14], the results of this study suggested that dietary intake of pure DPCs prevents fat accumulation in WAT, and that DPCs are a significant factor in the anti-obesity effects of PJT. In addition, the results suggested that DPCs' anti-obesity properties are caused by an increase in energy expenditures resulting from increased UCPs, and that PLGA-based nanoparticulate system is a powerful tool to enhance DPC activities.…”

“…Thus, these results suggested that the dietary intake of DPCs can induce the conversion of hypertrophic into hyperplastic obesity in HFD-fed mice. It has also suggested that other ingredients in PJT can exert antilipogenic effects on WAT because our previous study observed significant decreases in mRNA levels of lipogenesis-related genes in the epididymal WAT of mice fed HFD containing DPC concentrates from PJT (DPC contents~62.2%) [14]. In addition, it should be noted that these phenomena were observed in the low dose group, in which mice were fed 1% of the amount of DPCs fed to the regular dose group (Table 1).…”

“…The PJT leaves used in this study were cultivated and harvested on Yonaguni, an island in Okinawa Prefecture, Japan. DPC concentrate was prepared from the dried PJT leaves according to the methods used in our previous study [14]. The primary concentrate was further concentrated using a Hi-Flash column ODS-MS (YANAZAN Corp., Osaka, Japan), and subsequently evaporated.…”

“…Following 1-week acclimation, mice were randomly divided into the following four groups (n = 6 for each group): control, regular dose DPC (regular dose), low dose DPC (low dose), and low dose nanoparticulated DPC (nano-DPC) groups (Table 1). Experimental diets and DPC doses were optimized according to our previous study [14]. The experimental HFD containing 20% fat was prepared as the AIN-76 formulation and its composition is summarized in Supplementary Table S1.…”

“…The leaves of this plant have been used as a leafy vegetable and garnish and are well-known as "Choumeisou" in Japanese, which denotes a long-life herb in Okinawa, Japan. The results of our previous studies [14][15][16] have suggested that dietary intake of PJT ameliorates obesity and diabetes symptoms in mice fed a high-fat diet (HFD) and revealed that several types of dihydropyranocoumarins (DPCs) from PJT have inhibitory effects on lipid accumulation of differentiated 3T3-L1 adipocytes. We have also shown that dietary intake of DPC concentrate (puritỹ 60%) prepared from PJT significantly decreases the relative weight of white adipose tissue (WAT) in HFD-fed mice; therefore, we suggested that DPCs could be an anti-obesity compound of PJT [14], although these findings have not excluded the possibility that other impurities in DPC concentrate also have anti-obesity effects.…”