In Vivo and in Vitro Measurements of Protein Turnover

Bergen, Werner G.; Mulvaney, D. R.; Skjaerlund, D. M.; Johnson, Sheila; Merkel, R. A.

doi:10.1093/ansci/65.suppl_2.88

Cited by 10 publications

(33 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Since the turnover rate of muscle proteins in fast skeletal muscle is approximately half that of slow muscle, differences in individual muscles in synthesis and degradation rates can possibly be attributed to the differences in fiber type population (LAURENT et al 1978;MILLWARD, 1980). While the method for estimation of muscle protein synthesis and degradation used in this study allows examination of relative differences between treatments, direct comparisons of synthesis to degradation are not recommended (BERGEN et al 1987). In vitro protein degradation rates have been demonstrated to be higher and in vitro synthesis rates often lower than those estimated from in vivo methods (PALMER et al 1981;GOLDSPINK et al 1983).…”

Section: Discussionmentioning

confidence: 99%

Skeletal muscle infrastructure and protein turnover of lambs fed a diet low in selenium and vitamin E

Gore

McCarthy

Mulvaney

et al. 1990

Animal Physiology Nutrition

Self Cite

View full text Add to dashboard Cite

Zusammenfassung Der Proteinabbau bzw. die Proteinsynthese sowie die Ultrastruktur bei Skelettmuskeln wurde an 14 Suffolk‐Dorset‐Coopworth‐Lämmern bei unterschiedlicher Selen‐ bzw. Vitamin E‐Versorgung untersucht. Geprüft wurden Leber‐, Herz‐, Nieren‐ und Skelettmuskel hinsichtlich des Selengehaltes. Bei niedriger Se‐ und Vitamin E‐Versorgung wurden im Blut und in allen Geweben niedrigere Se‐Gehalte festgestellt. Obwohl keine Weiß‐Muskel‐Krankheit diagnostiziert werden konnte, zeigten sich im elektronenmikroskopischen Bild bei mangelhafter Se‐ und Vitamin E‐Versorgung charakteristische Degenerationserscheinungen bis zur Muskeldystrophy. Veränderungen im Proteinstoffwechsel der Muskel konnten nicht festgestellt werden. Aus den Ermittlungen muß abgeleitet werden, daß der Se‐ und Vitamin E‐Versorgung bei Lämmern größere Beachtung gewidmet werden muß. Darüberhinaus scheint das Lamm als Modelltier für Untersuchungen zur Muskeldegeneration geeignet zu sein.

show abstract

Section: Discussionmentioning

confidence: 99%

Skeletal muscle infrastructure and protein turnover of lambs fed a diet low in selenium and vitamin E

Gore

McCarthy

Mulvaney

et al. 1990

Animal Physiology Nutrition

Self Cite

View full text Add to dashboard Cite

show abstract

“…The pigs were weighed and blood samples were collected at weekly intervals for determination of serum concentrations of testosterone. Fractional protein synthesis rates ( FSR) were measured in skeletal muscle at 1, 2, and 4 wk by continuous infusion of radiolabeled tyrosine (Bergen et al, 1987). The pigs were removed from their dams during the 6-h infusion period and then immediately euthanatized ( 5 mL, 50% wt/vol phenobarbitol) and exsanguinated.…”

Section: Introductionmentioning

confidence: 99%

“…The left semitendinosus, longissimus, brachialis, and triceps brachii muscles were removed, dissected free from fat, and weighed. A subsample of the left semitendinosus muscle was frozen immediately in liquid nitrogen and stored at -80°C for determination of in vivo FSR (Mulvaney et al, 1985;Bergen et al, 1987).…”

Section: Introductionmentioning

confidence: 99%

“…At slaughter, muscle strips were teased free and removed from the right semitendinosus muscle for determination of in vitro protein synthesis rates according to the procedure described by Bergen et al (1987) and Skjaerlund et al (1988). The soft tissues of the left side were dissected free from skin and bone and then ground along with the muscles listed above for determination of total soft tissue protein.…”

Section: Introductionmentioning

confidence: 99%

“…Determination of In Vivo Fractional Protein Synthesis Rate. The FSR were determined according t o procedures described by Mulvaney et al (1985) and Bergen et al (1987). Twenty-four hours before infusion, pigs were anesthetized with halothane and catheters were surgically inserted into the right and left jugular veins.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Skeletal muscle growth and protein turnover in neonatal boars and barrows

Dr²,

Wg³

et al. 1994

Journal of Animal Science

Self Cite

View full text Add to dashboard Cite

The objective of this study was to determine the effect of castration, within 24 h after birth, on skeletal muscle growth and protein metabolism in neonatal pigs at 1, 2, and 4 wk of age. Four additional pigs were slaughtered at birth to obtain initial body composition. All other pigs were infused with [14C]tyrosine for 6 h before slaughter to determine in vivo fractional protein synthesis rates (FSR). At slaughter, muscle bundles were removed from the semitendinosus and incubated with [3H]tyrosine to determine in vitro protein synthesis rates. Nucleic acids and protein were determined on the semitendinosus muscle. Testosterone concentrations, determined at weekly intervals, peaked in boars at 3 wk of age. Castration at birth did not affect combined weights of the semitendinosus, longissimus, triceps brachii, and brachialis muscles. Likewise, neither in vitro protein synthesis rates nor in vivo FSR were affected by castration. However, a developmental decline in in vivo FSR and in vitro protein synthesis rates occurred from 1 to 4 wk. Neither concentrations nor total quantity of protein, RNA, or DNA in the semitendinosus muscle differed between neonatal boars and barrows at any age. Concentrations of DNA and RNA at 4 wk were two- and threefold lower, respectively, than at birth. Protein:DNA and protein:RNA ratios increased three- and sixfold, respectively, from birth to 4 wk. Testosterone concentrations had little effect on skeletal muscle growth and protein turnover rates during this neonatal period.

show abstract

Amino Acids in Beef Cattle Nutrition and Production

Bergen

2021

Advances in Experimental Medicine and Biology

View full text Add to dashboard Cite

In Vivo and in Vitro Measurements of Protein Turnover

Cited by 10 publications

References 0 publications

Skeletal muscle infrastructure and protein turnover of lambs fed a diet low in selenium and vitamin E

Skeletal muscle infrastructure and protein turnover of lambs fed a diet low in selenium and vitamin E

Skeletal muscle growth and protein turnover in neonatal boars and barrows

Amino Acids in Beef Cattle Nutrition and Production

Contact Info

Product

Resources

About