In Vivo Analysis of Rhizosphere Enzyme Activities by the Use of Plastic Syringes

Li, Ru-Jie; Wang, Yihan; Cai, Jie; Liu, Jie; Xie, Zhiping; Staehelin, Christian

doi:10.1007/978-981-13-5767-1_2

Cited by 1 publication

(2 citation statements)

References 15 publications

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“…Five-day-old M. truncatula seedlings grown on BNM agar plates were transferred to 1-mL plastic syringes ( Li et al., 2019 ). The syringes were filled with 300 μL BNM containing 0.5% (v/v) DMSO and 0.5 μM NodSm-IV(C16:2, S) to activate Nod factor signaling.…”

Section: Methodsmentioning

confidence: 99%

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The Medicago truncatula hydrolase MtCHIT5b degrades Nod factors of Sinorhizobium meliloti and cooperates with MtNFH1 to regulate the nodule symbiosis

Zhang

Fan

et al. 2022

Front. Plant Sci.

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Nod factors secreted by nitrogen-fixing rhizobia are lipo-chitooligosaccharidic signals required for establishment of the nodule symbiosis with legumes. In Medicago truncatula, the Nod factor hydrolase 1 (MtNFH1) was found to cleave Nod factors of Sinorhizobium meliloti. Here, we report that the class V chitinase MtCHIT5b of M. truncatula expressed in Escherichia coli can release lipodisaccharides from Nod factors. Analysis of M. truncatula mutant plants indicated that MtCHIT5b, together with MtNFH1, degrades S. meliloti Nod factors in the rhizosphere. MtCHIT5b expression was induced by treatment of roots with purified Nod factors or inoculation with rhizobia. MtCHIT5b with a fluorescent tag was detected in the infection pocket of root hairs. Nodulation of a MtCHIT5b knockout mutant was not significantly altered whereas overexpression of MtCHIT5b resulted in fewer nodules. Reduced nodulation was observed when MtCHIT5b and MtNFH1 were simultaneously silenced in RNA interference experiments. Overall, this study shows that nodule formation of M. truncatula is regulated by a second Nod factor cleaving hydrolase in addition to MtNFH1.

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Section: Methodsmentioning

confidence: 99%

“…Five-day-old M. truncatula seedlings grown on BNM agar plates were transferred to 1-mL plastic syringes (Li et al, 2019).…”

Section: Hydrolysis Of Nod Factors By M Truncatula Seedlingsmentioning

confidence: 99%

The Medicago truncatula hydrolase MtCHIT5b degrades Nod factors of Sinorhizobium meliloti and cooperates with MtNFH1 to regulate the nodule symbiosis

Zhang

Fan

et al. 2022

Front. Plant Sci.

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View full text Add to dashboard Cite

show abstract

In Vivo Analysis of Rhizosphere Enzyme Activities by the Use of Plastic Syringes

Cited by 1 publication

References 15 publications

The Medicago truncatula hydrolase MtCHIT5b degrades Nod factors of Sinorhizobium meliloti and cooperates with MtNFH1 to regulate the nodule symbiosis

The Medicago truncatula hydrolase MtCHIT5b degrades Nod factors of Sinorhizobium meliloti and cooperates with MtNFH1 to regulate the nodule symbiosis

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