In vivo 2D magnetic resonance spectroscopy of small animals

Méric, P.; Autret, Gwennhaël; Doan, Bich‐Thuy; Gillet, Brigitte; Sebrié, Catherine; Belœil, Jean‐Claude

doi:10.1007/s10334-004-0084-2

Cited by 18 publications

(12 citation statements)

References 128 publications

(212 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A recent review of the application to small animals is given by Méric et al (8). JPRESS has been applied to the human brain (4,(9)(10)(11)(12)(13)(14)(15)(16), muscle (3,17), prostate (18,19) and breast (20).…”

Section: Introductionmentioning

confidence: 99%

Improved two‐dimensional J‐resolved spectroscopy

et al. 2006

View full text Add to dashboard Cite

Localised two-dimensional J-resolved spectroscopy (JPRESS) is optimised for the in vivo detection of J-coupled metabolites using magnetic resonance spectroscopy at 3 T. The acquisition of echo signals starts as early as possible (i.e. maximum-echo sampling). This sampling scheme increases sensitivity and decreases overlap of peak tails, hence alleviating baseline problems. Reconstruction issues are discussed and the sensitivity is compared analytically with that of 1D PRESS. The qualitative behaviour of eddy currents in JPRESS is outlined and a 2D eddy current correction procedure based on the 1D phase deconvolution method is proposed.

show abstract

Section: Introductionmentioning

confidence: 99%

Improved two‐dimensional J‐resolved spectroscopy

et al. 2006

View full text Add to dashboard Cite

show abstract

“…2D MRS overcomes the sideband artifacts and is capable of resolving overlapping J-coupled resonances through the addition of a second spectral dimension that is indirectly detected through acquisition of multiple 1D MRS with incrementally longer TEs (25)(26)(27)(28). In a standard spin-echo based 1D MRS sequence there are three slice selective RF pulses (908-1808-1808).…”

mentioning

confidence: 99%

Combined DCE‐MRI and single‐voxel 2D MRS for differentiation between benign and malignant breast lesions

et al. 2010

View full text Add to dashboard Cite

Dynamic contrast-enhanced (DCE) magnetic resonance imaging (MRI) and proton (1H) magnetic resonance spectroscopy (MRS) provide structural and biochemical information, including vascular volume, vascular permeability and tissue metabolism. In this study, we performed analysis of the enhancement characteristic from DCE-MRI and the biochemical information provided by two-dimensional (2D) Localized Correlated Spectroscopy (L-COSY) MRS to determine the sensitivity and specificity of using DCE-MRI alone compared to the combination with 2D MRS. The metabolite ratios from the 2D MRS spectra were analyzed using multivariate statistical analyses to determine a method capable of automatic separation of the patient cohort into malignant and benign lesions. A total of 24 lesions were studied with 21 diagnosed accurately using the enhancement characteristics alone resulting in sensitivity and specificity of 100% and 73%, respectively. Analysis of the 2D MRS data demonstrated a significant difference (p < 0.05) in 12 of 18 metabolite ratios analyzed for malignant compared to benign lesions. Previous research focused on utilizing the choline signal to noise ratio (SNR) as a marker for malignancy has been verified using 2D MRS in this study. Using Fisher's linear discriminant test using water (WAT)/olefinic fat diagonal (UFD), choline (CHO)/fat (FAT), CHO/UFD, and FAT/methyl fat (FMETD) as predictors the sensitivity and specificity increased to 92% and 100%, respectively. Using the Classification and Regression Tree (CART) statistical analysis the resulting sensitivity and specificity were 100% and 91%, respectively, with the most accurate predictor for differentiating malignant and benign determined to be FAT/FMETD. The cases within the study that presented a indeterminate diagnosis using DCE-MRI alone were able to be accurately diagnosed when the metabolic information from 2D MRS was incorporated. The results suggest improved breast cancer detection through the combination of morphological and enhancement information from DCE-MRI and metabolic information from 2D MRS.

show abstract

“…To gain better spectral resolution and more rigorous metabolite assignment, various 2D NMR techniques have been applied to metabolite analysis in vivo in live animals [260]. In particular, 2D 1 H{ 13 C}-ge-HMQC was employed to investigate 13 C 1 -1-glucose metabolism in cat brain in vivo [261].…”

Section: Applications Of Nmr To Metabolomicsmentioning

confidence: 99%

“…The 13 C-edited 2D 1 H spectrum acquired in vivo from a cat brain resolves resonances of 13 C-4-Glu (4a) from 13 C-4-Gln (4b), which cannot be achieved with 1D 1 H methods. Also resolved are resonances of 13 C-3-Glu/Gln (5), 13 C-2-Glu/Gln (3), 13 C-1-α-glucose, and 13 C-1-β-glucose; the latter three would be difficult to detect without the better water suppression afforded by the 2D method [260]. Excerpted from MAGMA, 17 (2004) 317–338, In vivo 2D magnetic resonance spectroscopy of small animals, P. Meric, G. Autret, B.T.…”

Section: Figmentioning

confidence: 99%

Applications of NMR spectroscopy to systems biochemistry

Fan

Lane

2016

Progress in Nuclear Magnetic Resonance Spectroscopy

176

170

View full text Add to dashboard Cite

The past decades of advancements in NMR have made it a very powerful tool for metabolic research. Despite its limitations in sensitivity relative to mass spectrometric techniques, NMR has a number of unparalleled advantages for metabolic studies, most notably the rigor and versatility in structure elucidation, isotope-filtered selection of molecules, and analysis of positional isotopomer distributions in complex mixtures afforded by multinuclear and multidimensional experiments. In addition, NMR has the capacity for spatially selective in vivo imaging and dynamical analysis of metabolism in tissues of living organisms. In conjunction with the use of stable isotope tracers, NMR is a method of choice for exploring the dynamics and compartmentation of metabolic pathways and networks, for which our current understanding is grossly insufficient. In this review, we describe how various direct and isotope-edited 1D and 2D NMR methods can be employed to profile metabolites and their isotopomer distributions by stable isotope-resolved metabolomic (SIRM) analysis. We also highlight the importance of sample preparation methods including rapid cryoquenching, efficient extraction, and chemoselective derivatization to facilitate robust and reproducible NMR-based metabolomic analysis. We further illustrate how NMR has been applied in vitro, ex vivo, or in vivo in various stable isotope tracer-based metabolic studies, to gain systematic and novel metabolic insights in different biological systems, including human subjects. The pathway and network knowledge generated from NMR- and MS-based tracing of isotopically enriched substrates will be invaluable for directing functional analysis of other ‘omics data to achieve understanding of regulation of biochemical systems, as demonstrated in a case study. Future developments in NMR technologies and reagents to enhance both detection sensitivity and resolution should further empower NMR in systems biochemical research.

show abstract

In vivo 2D magnetic resonance spectroscopy of small animals

Cited by 18 publications

References 128 publications

Improved two‐dimensional J‐resolved spectroscopy

Improved two‐dimensional J‐resolved spectroscopy

Combined DCE‐MRI and single‐voxel 2D MRS for differentiation between benign and malignant breast lesions

Applications of NMR spectroscopy to systems biochemistry

Contact Info

Product

Resources

About