In vivo 2D 1H NMR of mdx mouse muscle and myoblast cells during fusion: Evidence for a characteristic signal of long chain fatty acids

Gillet, Brigitte; Doan, Bich‐Thuy; Verre-Serrie, C.; Barbere, B.; Berenger, G.; Morin, S.; Koenig, Jeanine; Pérès, M.; Sebille, Alain; Belœil, Jean‐Claude

doi:10.1016/0960-8966(93)90091-w

Cited by 16 publications

(21 citation statements)

References 18 publications

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“…However, there are no lipid deposits intracellularly or interstitially in the mdx TA (judged by PAS staining and routine light microscopy), which still exhibits the lipid signal. Therefore, the greater lipid content could be due to membrane destruction 40 in mdx, an extra linolenic-like fatty acid peak in regenerating mdx myofibers 25 (that cross-peak was not observed by two-dimensional COSY experiments of our in vitro DIA or TA samples), mobile neutral lipid domains intercalated within the bilayer lipid, 41,42 or intracellular lipid droplets as observed by electron microscopy. 13,43 There was a clear relationship between spectral properties of the proton resonances in affected muscle and cytological markers of the disease.…”

Section: Growth/dystrophy Studymentioning

confidence: 78%

“…Mdx dystrophy has been studied by in vivo NMR, with the finding of an added lipid resonance in two-dimensional spectra of young mdx mice, suggested to originate during myoblast fusion. 25 However, the reported NMR studies have not examined spectroscopic features that might best track functional outcomes, namely the stages of muscle growth, the progression of dystrophy and the muscle repair sequelae. Additionally, no NMR studies have reported any treatment effects on dystrophy.…”

Section: Introductionmentioning

confidence: 99%

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Nuclear magnetic resonance spectroscopy study of muscle growth,mdx dystrophy and glucocorticoid treatments: correlation with repair

et al. 1998

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Section: Growth/dystrophy Studymentioning

confidence: 78%

Section: Introductionmentioning

confidence: 99%

Nuclear magnetic resonance spectroscopy study of muscle growth,mdx dystrophy and glucocorticoid treatments: correlation with repair

et al. 1998

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“…63 Duchenne muscular dystrophy has also been investigated in intact cardiac tissue using high resolution magic angle spinning (MAS) NMR, coupled with PR. 68 Changes in lipid resonances together with increases in lactate and threonine were observed. Changes in lipid composition in mdx skeletal muscle have previously been identified in vivo and in intact biopsy samples.…”

Section: Metabonomic Studies Of Physiological Variationmentioning

confidence: 96%

“…Changes in lipid composition in mdx skeletal muscle have previously been identified in vivo and in intact biopsy samples. 68,69 Given the extensive use of genetically-modified mice, it is important to be aware of the strain background in order to fully characterize the changes induced by genetic deletion or modification. 70,71 For instance, the Fcer1g mouse comes on both a Balbc and C57BL/6J background.…”

Section: Metabonomic Studies Of Physiological Variationmentioning

confidence: 99%

NMR-based metabonomic approaches for evaluating physiological influences on biofluid composition

et al. 2004

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Strategies such as genomics, proteomics and metabonomics are being applied with increasing frequency in the pharmaceutical industry. For each of these approaches, toxicological response can be measured by terms of deviation from control or baseline status. However, in order to accurately define drug-induced response, it is necessary to characterize the normal degree of physiological variation in the absence of stimuli. Here, 1 H NMR spectroscopic-based analyses of the metabolic composition of urine in experimental animals under various normal physiological conditions are reviewed. In particular, the effects of inter-animal and diurnal variation, gender, age, diet, species, strain, hormonal status and stress on the biochemical composition of urine are explored. Pattern recognition methods facilitate the comparison of urine NMR spectra over a given time-course, enabling the establishment of changes in profile and highlighting the dynamic metabolic status of an organism. Thus metabonomic approaches based on information-rich spectroscopic data sets can be used to evaluate normal physiological variation and for investigation of drug safety issues.

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“…These included changes in the lipid components of membranes (Gillet et al, 1993), pH and energy metabolism using proton and phosphorous NMR spectroscopy in muscle (Dunn et al, , 1992Radda, 1999) and/or brain (Rae et al, 1998;Tracey et al, 1996a,b), and treatment responses that were observed in skeletal muscle including the diaphragm, and in cardiac muscle (Anderson et al, 1998McIntosh et al, 1998aMcIntosh et al, ,b,c, 1994Skrabek and Anderson, 2001).…”

Section: A U T H O R ' S P E R S O N a L C O P Ymentioning

confidence: 99%

Methods and biomarkers for the diagnosis and prognosis of cancer and other diseases: Towards personalized medicine

Anderson

Hansen

Mooren

et al. 2006

Drug Resistance Updates

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The rapid development of new diagnostic procedures, the mapping of the human genome, progress in mapping genetic polymorphisms, and recent advances in nucleic acid-and protein chip technologies are driving the development of personalized therapies. This breakthrough in medicine is expected to be achieved largely due to the implementation of "lab-on-the-chip" technology capable of performing hundreds, even thousands of biochemical, cellular and genetic tests on a single sample of blood or other body fluid. Focusing on a few disease-specific examples, this review discusses selected technologies and their combinations likely to be incorporated in the "lab-on-the-chip" and to provide rapid and versatile information about specific diseases entities. Focusing on breast cancer and after an overview of single-nucleotide polymorphism (SNP)-screening methodologies, we discuss the diagnostic and prognostic importance of SNPs. Next, using Duchenne muscular dystrophy (DMD) as an example, we provide a brief overview of powerful and innovative integration of traditional immuno-histochemistry techniques with advanced biophysical methods such as NMR-spectroscopy or Fourier-transformed infrared (FT-IR) spectroscopy. A brief overview of the challenges and opportunities provided by protein and aptamer microarrays follows. We conclude by highlighting novel and promising biochemical markers for the development of personalized treatment of cancer and other diseases: serum cytochrome c, cytokeratin-18 and -19 and their proteolytic fragments for the detection and quantitation of malignant tumor mass, tumor cell turn-over, inflammatory processes during hepatitis and Epstein-Barr virus (EBV)-induced hemophagocytic lymphohistiocytosis and apoptotic/necrotic cancer cell death.

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In vivo 2D 1H NMR of mdx mouse muscle and myoblast cells during fusion: Evidence for a characteristic signal of long chain fatty acids

Cited by 16 publications

References 18 publications

Nuclear magnetic resonance spectroscopy study of muscle growth,mdx dystrophy and glucocorticoid treatments: correlation with repair

Nuclear magnetic resonance spectroscopy study of muscle growth,mdx dystrophy and glucocorticoid treatments: correlation with repair

NMR-based metabonomic approaches for evaluating physiological influences on biofluid composition

Methods and biomarkers for the diagnosis and prognosis of cancer and other diseases: Towards personalized medicine

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