In Vitro α-Glucosidase Inhibition and Antioxidant Activity of Mulberry (Morus Alba L.) Leaf Ethanolic Extract

Danuri, Hasim; Lestari, Widia Ayu; Sugiman, Uci; Faridah, Didah Nur

doi:10.25182/jgp.2020.15.1.45-52

Cited by 5 publications

(6 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The antioxidant activity of red betel leaf extract compared to the antioxidant activity of noni and Physalis peruviana, ethanol, and n-hexane solvents of red betel leaf was better than noni (Morinda citrifolia L.) extracted with methanol 33 because red betel leaf extract showed no effect prooxidant. Another similar study showed no different results, namely mulberry ethanol extract at a concentration of 200 μg/mL (200 ppm) with an inhibition of 44.19% (Danuri et al, 2020). According to Alfarabi (2010), red betel leaf at a concentration of 200 mg/10 mL with water as a solvent was able to inhibit MDA formation by 81.78%; when compared to this study, 70% ethanol extract and n-hexane extract of red betel leaves had lower antioxidant activity.…”

Section: Discussionmentioning

confidence: 87%

Antioxidant Activity and Tyrosinase Inhibitor of Red Betel Leaf Extract

Weni,

Safithri

2023

IJAR

View full text Add to dashboard Cite

Red betel leaf (Piper Crocatum) contains flavonoids, tannins, steroids, and alkaloids, known to act as antioxidants. This study aimed to determine the antioxidant activity of red betel leaf extract. The solvents used for the extraction process are ethanol and n-hexane. The thiobarbituric acid (TBA) method was used to determine antioxidant activity. The results showed that the extract of 200 ppm inhibited fatty acid oxidation by 52.13%. No significant difference (α = 0.05) inhibition of unsaturated fatty acids oxidation between the sample and α-tocopherol at 200 ppm. The ethanol extract of red betel can inhibit the tyrosinase enzyme higher than the n-hexane extract of red betel, as seen from the IC50 value of the ethanol extract of red betel of 1655 ppm, while the IC50 value of n-hexane of red betel is 3090.56 ppm.

show abstract

Section: Discussionmentioning

confidence: 87%

Antioxidant Activity and Tyrosinase Inhibitor of Red Betel Leaf Extract

Weni,

Safithri

2023

IJAR

View full text Add to dashboard Cite

show abstract

“…White mulberry leaf and green apple peel are two natural ingredients, that have shown to reduce postprandial blood glucose levels by regulating the digestion of the carbohydrates and by modulating the uptake of glucose at the brush border of the intestine. The individual capabilities were demonstrated in various randomised clinical studies [11][12][13][14]16,18,19,[21][22][23][24][25][26] . In this randomised, placebocontrolled, crossover study, carried out in healthy normoglycaemic individuals, we have demonstrated the efficacy of a combined extract of white mulberry leaf and green apple peel (Glubloc™) on postprandial glucose and insulin levels.…”

Section: Discussionmentioning

confidence: 99%

“…Mulberry leaf extract not only directly affects α-glucosidase and related enzymes, and influence carbohydrate metabolism and absorption 13,24 . Its ability to moderate insulin spikes is noteworthy, given the direct correlation between elevated systemic insulin levels and increased whole-body glucose uptake 27 .…”

Section: Discussionmentioning

confidence: 99%

“…Several clinical studies have shown the positive benefit of mulberry leaf extracts on postprandial sugar spikes in healthy as well as diabetic individuals [10][11][12] . The active component of mulberry extract is 1-DNJ (imniosugars) and alkaloids, work by temporarily blocking the enzymes pancreatic alpha glucosidase and amylase 13,14 . Acarbose, an amino oligosaccharide isolated from the fermentation broth of Actinoplanes sp., is another product that has a similar mechanism of action i.e., inhibits brush-border α-glucosidases in humans.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

A Novel Nutritional Supplement Reduces Postprandial Glucose Response in Healthy Individuals in a Randomised, Placebo-Controlled, Crossover Clinical Study

Guntupalli,

Karpuram,

et al. 2024

Preprint

View full text Add to dashboard Cite

Background: Sugar laden foods and carbohydrate rich diets with high glycaemic index intake are associated with weight gain, increased incidence of diabetes and is linked with increased cardiovascular risk. A blend of mulberry leaf extract and apple peel extract (Glubloc™) may reduce postprandial blood glucose surge and insulin response following dietary carbohydrate intake by limiting its digestion and uptake from the gut. Methods: In this randomised, placebo-controlled, crossover study, thirty healthy male and female subjects were supplemented with either placebo or Glubloc™, 10 min before a carbohydrate and sugar rich meal intake. Changes in postprandial blood glucose (upto120 minutes) and insulin levels (at 60 minutes) were measured as the primary endpoints. Changes in blood glucose incremental area under the concentration curve (iAUC), maximum concentration (Cmax) and peak time (Tmax) were compared. Results: Thirty adults (mean age 31±7.2 years, BMI 24±3.8 kg/m2) completed the study and the Glubloc™ blend relative to placebo significantly reduced glucose iAUC at 0.5 h (− 38%, p < 0.0003), 1.0 h (− 30%, p = 0.0007), 1.5 h (− 29%, p = 0.0008), and 2.0 h (− 26%, p = 0.002). A statistically significant reduction in 1 h insulin levels (− 35%, p = 0.029) was observed. Conclusion: A single dose supplementation of Glubloc™ significantly reduced the postprandial blood glucose spike and serum insulin levels. The observation of a significant glucose reduction paralleled with a significant lower insulin response supports a reduced gastrointestinal glucose absorption. None of the subjects experienced any major gastrointestinal side effects. This study builds a body of evidence that the combination of white mulberry leaf and green apple peel extracts (Glubloc™) can be a useful supplement to manage postprandial glucose levels, further studies are necessary to evaluate its potential in type 2 diabetes and the regulation of dysglycaemia.

show abstract

“…Bitkilerde yaygın olarak bulunan diyet polifenollerinin antioksidan kapasitelerinin yanında, sözü geçen bu sindirim enzimlerine karşı inhibitör etkilerinin de olduğu bildirilmiştir (Xiao vd., 2013;Asgar, 2013). Literatürde farklı tür ve menşeili dut yapraklarının antioksidan ve hipoglisemik etkilerini inceleyen çalışmalar mevcuttur (Anwar vd., 2015;Hasim, Uci ve Didah, 2020;He vd., 2020;Iqbal vd., 2012;Levickiene vd., 2017;Thabti, Marzougui, Elfalleh ve Ferchichi, 2011;Yu vd., 2018). Bu çalışmalar incelendiğinde; dut yapraklarıyla yapılan antioksidan aktivite çalışmalarının toplam fenolik madde ve flavonoid tayinlerinin yanında özellikle DPPH veya ABTS radikallerini giderme aktivitesi ile sınırlı oldukları görülmektedir.…”

Section: Introductionunclassified

A Study on Antioxidant and Antidiabetic Activities of Mulberry Leaves Which Grown in Edirne Province

İşbilir

Çelik

2021

Journal of Advanced Research in Natural and Applied Sciences

View full text Add to dashboard Cite

Makale TarihçesiÖz − Dut yaprakları halk arasında çeşitli hastalıklarda geleneksel olarak kullanılan bir bitkidir. Bitkilerin gösterdikleri çok çeşitli biyolojik etkinlikleri içerdikleri bileşenlerden kaynaklanmakta olup, bitkinin fitokimyasal bileşimi ve biyolojik potansiyeli yetiştiği bölgeye göre değişiklik göstermektedir. Bu çalışmada Edirne ilinde yetişen beyaz ve siyah dut ağacı yapraklarından elde edilen su ve etanol ekstraktlarının antioksidan aktivitesinin ve in vitro enzim inhibisyon testleri ile antidiyabetik kapasitesinin belirlenmesi amaçlanmıştır. Beyaz ve siyah dut ağacı yaprak ekstraktlarının toplam fenolik madde miktarı 35.08-47.57 µg gallik asit eşdeğeri/mg, flavonoid miktarı 18.40-46.25 µg rutin eşdeğeri/mg ve tanen miktarı 4.59-7.53 µg tannik asit eşdeğeri/mg aralıklarında tayin edildi. Antioksidan aktivite testlerinde 1,1-difenil-2-pikrilhidrazil (DPPH) ve 2,2΄-azinobis-(3-etilbenzotiazolin-6-sülfonik asit) (ABTS) radikallerini gidermede (EC50 sırasıyla 0.31 mg/mL ve 0.79 mg/mL) ve CUPRAC metodunda (9.1 μmol troloks eşdeğeri antioksidan kapasite-TEAC) siyah dut yapraklarının etanol ekstraktı yüksek aktivite gösterirken, betakaroten ağartma metodunda beyaz dut yapraklarının etanol ekstraktı (EC50 0.47 mg/mL) daha iyi sonuç verdi. Dut yapraklarının sadece su ekstraktlarının metal iyonlarını şelatlama gücüne sahip olduğu görüldü. Antidiyabetik aktivite çalışmasında yaprakların su ekstraktları değişen oranlarda α-amilaz ve α-glukozidaz inhibisyonu gösterdi. Su ekstraklarının karbonhidrat sindirim enzimleri üzerine potansiyel inhibitör etkisi göstermesi, ülkemizde besin olarak tüketilmeyen dut yapraklarının ilaç hammaddesi kaynağı olarak değerlendirilebileceğini; siyah dut yapraklarının ise antioksidan katkı amaçlı olarak kozmetik uygulamalarda kullanılmak üzere ekonomiye kazandırılabileceğini düşündürmektedir.

show abstract

In Vitro α-Glucosidase Inhibition and Antioxidant Activity of Mulberry (Morus Alba L.) Leaf Ethanolic Extract

Cited by 5 publications

References 21 publications

Antioxidant Activity and Tyrosinase Inhibitor of Red Betel Leaf Extract

Antioxidant Activity and Tyrosinase Inhibitor of Red Betel Leaf Extract

A Novel Nutritional Supplement Reduces Postprandial Glucose Response in Healthy Individuals in a Randomised, Placebo-Controlled, Crossover Clinical Study

A Study on Antioxidant and Antidiabetic Activities of Mulberry Leaves Which Grown in Edirne Province

Contact Info

Product

Resources

About