In vitro α-amylase and α-glucosidase Inhibition, Antioxidant, Anti- Inflammatory Activity and GC-MS Profiling of Avicennia alba Blume

Abstract

Background: Avicennia alba Blume, is a well-known mangrove plant used in traditional medicinal practices for several human ailments. Objective: The study aimed at evaluation of antidiabetic, antioxidant, anti-inflammatory and cytotoxic activities of A. alba ethanolic leaf (AAL) and bark (AAB) extract along with phytochemical investigation. Methods: In vitro antidiabetic study was done by α-amylase, α-glucosidase enzyme inhibition assay; antioxidant study by DPPH, ABTS, superoxide, and metal chelating assays, anti-inflammatory study by protein denaturation assay. The cytotoxicity study was done on TC1 murine cell line. Further, GCMS analysis was carried out for AAL extracts. Results: AAL exhibited better antidiabetic activities with IC50 values of 1.18 and 0.87 mg/ml against α-amylase and α-glucosidase enzymes respectively. The AAL exhibited better ABTS, superoxide scavenging and metal chelating potential with IC50 values of 0.095, 0.127 and 0.444 mg/ml. However, AAB showed higher DPPH scavenging potential with IC50 value of 0.163 mg/ml. The AAL also exhibited higher protein denaturation potential with IC50 value of 0.370 mg/ml. The bark extract exhibited better cytotoxic activity as compared to leaf extracts on the TC1 murine cell line. The phytochemical study revealed higher total phenol (25.64 mg GAE/g), flavonoid (205.09 mg QE/g), and tannin content (251.17 mg GAE/g) in AAL. The GC-MS analysis revealed presence of several compounds in AAL extract. Conclusion: The result of the present study highlights the antidiabetic, antioxidant and cytotoxic activities of mangrove plant Avicennia alba.

GCMS and FTIR analysis of ethanol and methanol leave extract of Urena lobata (Caesar weed) for bioactive phytochemical constituents

“…For example, 1-Eicosanol, an alcohol has been reported to possess antimalarial, antifungal, and antioxidant activities 31 while Methyl stearate has proven to be a potent anti-inflammatory,intestinal lipid metabolic regulator, GABA aminotransferase inhibitor,antinociceptive, anthelmintic, and a potent gastric inhibitor 42 . On the other hand, Dodecyl acrylate, a typical acrylate ester is known to be antioxidant and antimicrobial 36 while 6,11-Dimethyl-2,6,10 dodecatrien-1ol has been researched to possess antimicrobial activities 46 .…”

In Vitro Alpha-Glucosidase Inhibitory Effect of Etlingera Elatior Ethanol Extract Growing in Gayo Highland, Aceh Province, Indonesia

“…44 In addition, alkaloids, flavonoid glucosides, and saponins, which are also found in E. elatior flowers, are among other potential hypoglycemic agents that play a crucial role in the prevention of diabetes by hindering α-glucosidase enzyme activity. 29,[45][46][47][48] In Indonesian society, young flowers of this plant have been widely used as food ingredients in traditional dishes and empirically used as traditional medicine. 16 In addition to the leaves and flowers, the extract of fruit was another E. elatior plant part effectively hindering α-glucosidase activity, with percent inhibition of almost 40.0% and IC 50 value of less than 50 μg/mL.…”