In vitro Xanthine Oxidase (XO) and Albumin Denaturation Inhibition Assay of Barringtonia racemosa L. and Total Phenolic Content Analysis for Potential Anti-Inflammatory Use in Gouty Arthritis

Abstract

Aim:This study was conducted to evaluate the in vitro anti-inflammatory activities and total phenolic content (TPC) of methanolic extracts of infloresence axes, endosperms, leaves, and pericarps of Barringtonia racemosa L.Methods:The anti-inflammatory study was conducted by assessing the potential through xanthine oxidase (XO) and albumin denaturation inhibition assays. Meanwhile, the TPC in the extracts were assessed by Folin-Ciocalteu assay.Results:In the XO inhibition assay, the infloresence axes extract was found to exert the highest inhibition capacity at 0.1% (w/v) with 59.54 ± 0.001% inhibition followed by leaves (58.82 ± 0.001%), pericarps (57.99 ± 0.003%), and endosperms (57.20 ± 0.003%) extracts. Similarly in the albumin denaturation inhibition assay, the infloresence axes extract had shown the greatest inhibition capacity with 70.58 ± 0.004% inhibition followed by endosperms (66.80 ± 0.024%), leaves (65.29 ± 0.006%), and pericarps extracts (43.33 ± 0.002%). Meanwhile, for TPC analysis, leaves extract was found to have the highest phenolic content (53.94 ± 0.000 mg gallic acid equivalent [GAE]/g DW) followed by infloresence axes (31.54 ± 0.001 mg GAE/g DW), endosperms (22.63 ± 0.001 mg GAE/g DW), and the least was found in pericarps (15.54 ± 0.001 mg GAE/g DW).Conclusion:The results indeed verified the in vitro anti-inflammatory activities of B. racemosa and supported its potential to be used in alleviating gouty arthritis and XO-related diseases.

In vitro biological activities of aqueous extracts of Tetrapleura tetraptera (Schumach. & Thonn.) taub. and Aframomum citratum (C. Pereira) K.Schum from three Agroecologic Zones in Cameroon

“…Inflammation is implicated in the pathogenesis of many diseases (arthritis, stroke, cancer, diabetes etc.) and protein denaturation, lipoxygenase activity are well correlated with the appearance of the inflammatory response [30]. The extracts from ZONE V are the most active and T. tetraptera extracts have at equal concentration the most effective activities.…”

Enzyme‐Assisted Extraction of Triterpenoid Saponins from Pseuderanthemum palatiferum (Nees) Radlk. Dry Leaf Powder and Bioactivities Examination of Extracts

“…The IC 50 value could not be determined because high extract concentration increases the shade of reaction solution. Additionally, ethanol partly precipitates albumin and forms complexes with several protein . Instead, IC 20 was calculated.…”

Evaluation of anti-inflammatory property of the roots of Borassus flabellifer

“…The IC 50 of REBF calculated to inhibit protein denaturation was found to be 2.1mg, and similar regression analysis shows that the IC 50 of diclofenac was 1.02 mgFigure 3. Denaturation of tissue proteins is correlated with pathological responses such as hypersensitivity, which leads to in lammatory diseases such as arthritis, and thus, any compound of plant origin which can inhibit protein denaturation will have signi icant therapeutic potential as an anti-in lammatory drug (Osman et al, 2016).…”