In vitro Wound Healing Efficacy of Silver Nanoparticles Synthesized from Aqueous Extract of Turbinaria conoides.

Abstract

The wound healing potentials of brown algae Turbinaria conoides aqueous extract (TCAe) and silver nanoparticles synthesized utilizing T. conoides aqueous extract (TCAgNPs) were investigated in this study. TCAgNPs and TCAe were tested for cytotoxicity on human dermal fibroblast cells using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, which revealed that TCAgNPs and TCAe were not cytotoxic and may be tested for medicinal qualities. TCAgNPs and TCAe were tested for wound healing efficacy using a wound scratch assay on human dermal fibroblast cells. The damaged cells were subjected to TCAgNPs and TCAe, which demonstrated stronger wound repair activities than the control (Untreated). The cell cycle study of human dermal fibroblast primary cell lines treated with TCAgNPs and TCAe, as well as those not treated, was performed using flow cytometry to determine the DNA content of the nuclei. These findings show that TCAgNPs-treated cells proliferated more than TCAe and control-treated cells, implying that cell proliferation is boosted, which aids the wound-healing process. During immunoblot analysis, the TCAgNPs-treated group showed higher collagen and fibronectin expression than the TCAe-treated group. Our findings imply that TCAgNPs and TCAe can repair wounds in vitro and could be used as a source of wound healing agents.