In vitro wound healing activity of Scrophularia striata hydroalcoholic extract

Haddadi, Rasool; Tamri, Pari; Jooni, F. Javani

doi:10.1016/j.sajb.2019.01.002

Cited by 18 publications

(9 citation statements)

References 14 publications

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“…In this study, we showed that SSE had no significant effect on MMP8 expression. Previous studies have shown the anti-inflammatory, antioxidant, and wound-healing properties of the hydroalcoholic extract of S. striata (28)(29)(30). Based on these findings, we examined the effect of the hydroalcoholic extract of S. striata on the modulation of ECM in vitro.…”

Section: Discussionmentioning

confidence: 94%

Modulation of Extracellular Matrix by Scrophularia striata Extract in Vitro: A Potential Antiscarring Agent

Tamri

Haddadi

Jouni

2020

Jundishapur J Nat Pharm Prod

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Background: Hypertrophic scars are the consequences of the aberration of normal wound healing. To date, therapeutic strategies for abnormal scarring have been unsuccessful. ‎‏The abnormal extracellular‎ matrix is one of the most important contributing factors to ‎hypertrophic scars. ‏Scrophularia striata has been used in Iranian folk medicine for the treatment of burn wounds. ‏The plant extract accelerates wound healing and attenuates scar formation. Objectives: The study was performed to investigate the effects of Scrophularia striata hydroalcoholic extract (SSE) on MMP1, MMP8, fibronectin, collagen type І, and total collagen produced by human skin fibroblasts in the culture medium. Methods: The effects of SSE on the expression of MMP1, MMP8, fibronectin, and collagen type І in human skin fibroblast (HSF) were evaluated using Q-PCR and Western blotting methods. In addition, the effect of SSE on the total collagen content was measured in cultured HSF using Red Sirius Kit. Results: SSE significantly induced the expression of MMP1 and suppressed the production of fibronectin at the mRNA and protein levels. The total collagen content was significantly lower in SSE-treated cells than in untreated cells. SSE did not have any significant effect on MMP8 and collagen type І expression. Conclusions: The results of this study revealed that SSE could modulate the extracellular matrix turnover and had the potential for the prevention and treatment of hypertrophic scars.

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Section: Discussionmentioning

confidence: 94%

Modulation of Extracellular Matrix by Scrophularia striata Extract in Vitro: A Potential Antiscarring Agent

Tamri

Haddadi

Jouni

2020

Jundishapur J Nat Pharm Prod

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“…This compound possesses anti-inflammatory, antioxidant, and antimicrobial properties (7,11,12). With potential for the wound-healing activity, CA is a key component of many plants such as Scrophularia striata (25,26), Cinnamon verum (27), Inula viscosa (28), and Stevia rebaudiana and may contribute to the enhancement of wound healing by these plants.…”

Section: Discussionmentioning

confidence: 99%

Investigation of Healing Effects of Cinnamic Acid in a Full-Thickness Wound Model in Rabbit

Naghavi

Tamri

Asl

2021

Jundishapur J Nat Pharm Prod

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Background: Wounds represent a major global problem for health care systems, clinicians, patients, and their families. Cinnamic Acid (CA) is a naturally occurring phenolic compound that possesses anti-inflammatory, antimicrobial, and antioxidant properties. Objectives: This study aimed to investigate the effects of CA on skin wound-healing in the animal model. Methods: Full-thickness wounds were created on the back of white New Zealand rabbits of both sexes. Animals were divided into six groups (six animals and 12 wounds in each group). Negative control received no treatment, while positive control was treated with phenytoin cream, vehicle group with eucerin, and test groups with 0.1, 1, and 10% CA ointments. The healing activity of CA was evaluated by determining the wound closure rate and hydroxyproline content of wound tissue samples. In addition, the histopathological study of tissue samples of different groups was performed using hematoxylin and eosin staining. Results: The rate of wound closure and hydroxyproline levels of tissue samples in animals treated with CA 0.1% were significantly (P < 0.05) higher than those of no-treatment and vehicle-treated groups. Histological study revealed the increased number of fibroblasts and hair follicles, increased reepithelialization rate, and enhanced neovascularization in CA 0.1%-treated group when compared to no-treatment and vehicle groups. Conclusions: Cinnamic acid at low concentrations (< 1%) is potent for skin wound-healing and could be used as a safe and effective topical healing agent. Further studies are needed to confirm our findings.

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“…A possible explanation is that the cells used in their study were keratinocytes, whereas the cells used in this study were fibroblasts. In another study, human fibroblast cell proliferation significantly increased when treated with Scrophularia striata at 10, 50 and 100 μg/mL after 48h and with at 50, 100 and 200 μg/ml after 72h [46].…”

Section: Cell Proliferation Of Sert and Rhodomyrtonementioning

confidence: 90%

Wound Healing Potential of Rhodomyrtus tomentosa and its Bioactive Compounds- Rhodomyrtone

Kamarudin

Halim

Anuar

et al. 2021

JPRI

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Aims: The present work was aimed to study the phytochemical composition of a crude ethanolic extract of Rhodomyrtus tomentosa [SERT], and the presence of rhodomyrtone and SERT's in vitro wound healing activity. Introduction: Rhodomyrtus tomentosa is native plant to southern and southeastern Asia, India, east to southern China, Taiwan, Philippines, and south to Malaysia. In the traditional Vietnamese, Chinese and Malaysia, all its part, including leaves, roots, buds, and fruits have been used. A need for a new source of wound healing agent is the call for the investigation of the potential of R. tomentosa as the source of health-promoting agent, specifically as a natural wound healing agent. Methodology: SERT was screen for its phytochemicals and the detection of rhodomyrtone using liquid chromatography–mass spectrometry, /Quadrupole time-of-flight [LC-MS/QTOF] analysis. Cell viability, cell proliferation, and migration assay were performed to examine the SERT effect's in vitro wound healing activity on human fibroblast cells [CRL-2522]. Results: The phytochemical study showed the presence of saponins, flavonoids, tannins and steroid in the crude ethanolic extract. The LC-MS analysis of crude ethanolic extract of SERT showed presents of rhodomyrtone which is one of the major compounds in the extract. SERT exhibit proliferative and migratory rate in human fibroblast cells [CRL 2522] in dose-dependent manner, which supports wound healing process. Its bioactive compounds presented wound healing activities at 0.325 up to 2.5 µg/mL. Conclusion: Both SERT and rhodomyrtone portrayed in vitro wound healing activities. Further studies to elucidate the mechanism of action of SERT and rhodomyrtone is recommended.

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In vitro wound healing activity of Scrophularia striata hydroalcoholic extract

Cited by 18 publications

References 14 publications

Modulation of Extracellular Matrix by Scrophularia striata Extract in Vitro: A Potential Antiscarring Agent

Modulation of Extracellular Matrix by Scrophularia striata Extract in Vitro: A Potential Antiscarring Agent

Investigation of Healing Effects of Cinnamic Acid in a Full-Thickness Wound Model in Rabbit

Wound Healing Potential of Rhodomyrtus tomentosa and its Bioactive Compounds- Rhodomyrtone

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