In vitro virucidal activity of a styrylpyrone derivative against herpes simplex virus strain KOS-1

Moses, Micheal; Nor, Norefrina Shafinaz Md; Ibrahim, Nazlina

doi:10.1063/1.4895262

Cited by 6 publications

(6 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The different concentrations of extract (1-31 µg/mL) were mixed with DMEM + methylcellulose immediately after the adsorption period and added into all test wells. The cells were then incubated for 48 h. After incubation, the cells were stained using crystal violet and plaques were counted according to published protocol [27].…”

Section: Post-treatment Of Hsv-1-infected Cells With Plant Extractmentioning

confidence: 99%

In vitro virucidal activity of Kyllinga nemoralis aqueous extract against herpes simplex virus

Wahab¹,

Ganasen²,

Rahman³

et al. 2022

J App Biol Biotech

View full text Add to dashboard Cite

Kyllinga nemoralis is an ignored plant distributed in tropical, subtropical, and warm temperature regions around the world. K. nemoralis plant is rich with bioactive molecules exhibiting antiherpetic properties. This study determined the antiviral activities of K. nemoralis stem with leaves aqueous extract against herpes simplex virus type 1 (HSV-1). The stem with leaves of K. nemoralis plant was extracted using aqueous extraction method. The cytotoxicity of K. nemoralis extract was evaluated using a cell viability assay. Plaque reduction assays were carried out to estimate the antiviral activity of K. nemoralis extract against HSV-1. Antiviral studies consisted of post-treatment, pre-treatment, and virucidal assay. Cell viability assay revealed K. nemoralis stem with leaves aqueous extract is non-toxic toward Vero cells. K. nemoralis stem with leaves aqueous extract inhibited HSV-1 replication in Vero cells with 50% effective concentration (EC 50 ) = 0.004 mg/mL and selective index (SI) = 14 when given after adsorption to the cells. The EC 50 against HSV-1 was 0.013 mg/mL and SI = 4.31 when cells were treated 24 h before virus infection. K. nemoralis stem with leaves aqueous extract exhibited direct virucidal activity against HSV-1 with EC 50 = 0.0065 mg/mL and SI = 8.62. The results presented the potential of K. nemoralis as an anti-HSV-1 agent by the following modes: direct damage to viral particle, interruption of virus attachment, and suppression of viral replication cycle.

show abstract

Section: Post-treatment Of Hsv-1-infected Cells With Plant Extractmentioning

confidence: 99%

In vitro virucidal activity of Kyllinga nemoralis aqueous extract against herpes simplex virus

Wahab¹,

Ganasen²,

Rahman³

et al. 2022

J App Biol Biotech

View full text Add to dashboard Cite

show abstract

“…Screening for antiviral activity was performed using 3 different treatments. 17 1) Post-treatment: To evaluate antiviral activity of extract against intracellular replication of DENV-2, cells were inoculated with virus 2 hour before treatment with extract. 2) Pre-treatment: In order to determine the prophylactic anti-HSV-1 activity of extract, virus was inoculated to cells 24 hours after treatment with extract.…”

Section: Antiviral Assaymentioning

confidence: 99%

Antiviral Activity of an Extract from Leaves of the Tropical Plant Cynometra cauliflora

Wahab¹,

Azizul²,

Badya³

et al. 2021

Self Cite

View full text Add to dashboard Cite

Background: Cynometra cauliflora is a species of tree in the family Fabaceae and has been used in folk medicinal preparation. Objectives: In this study, Cynometra cauliflora methanolic leaves extract was tested against clinical isolate herpes simplex virus type-1 (HSV-1). Materials and Methods: The leaves of C. cauliflora plant was extracted using methanol extraction method. Cytotoxicity was assessed using 3-(4,5-dimethylthiazol-2,5-diphenyltetrazolium bromide (MTT) assay. Plaque reduction assays were carried out to evaluate the antiviral activity of C. cauliflora extract against HSV-1. These include post-treatment, pre-treatment and virucidal assays. Results: The value of cytotoxic concentration, CC 50 of C. cauliflora extract was 36 mg/ mL. High antiviral activity was observed in post-treatment. C. cauliflora extract treatment was found to not interfere directly to infectious particle and confer mild protection when given as prophylaxis. Conclusion: This study provides important novel insights on the phytomedicinal properties of C. cauliflora extracts on HSV-1.

show abstract

“…On the other hand, SPD has shown potential anticancer activity and virucidal activity. 23,24 Cancer is a scenario where less apoptosis occurs, resulting in malignant cells that will not die. 25 Apoptosis is reduced when downregulation of p53 protein occurs, which result in enhanced tumour growth and development.…”

Section: Introductionmentioning

confidence: 99%

Styrylpyrone Derivative from Goniothalamus sp.: A Powerful Drug for Fighting Against Herpes Simplex Virus Type 1

Fouzi¹,

Wahab²,

Yan³

et al. 2021

Self Cite

View full text Add to dashboard Cite

Background:The emergence of drug resistance towards Herpes Simplex Virus Type 1 (HSV-1) has encouraged scientists to develop novel lower toxicity and highly effective anti-HSV drugs. Styrylpyrone derivative (SPD) is a bioactive compound isolated from the roots and leaves of Goniothalamus sp. It is believed that this compound possesses antiviral properties against HSV-1. Objective: This paper introduces the interaction of SPD towards HSV-1 through in silico study of molecular docking and molecular dynamic simulation. Materials and Methods: Molecular docking is a computational tool which is used to study the molecular interaction between two or more structures. ADME/T properties of the SPD were generated using the SwissADME online tool in which SPD was found to have a good pharmacokinetic profile. Results: Molecular docking study revealed that SPD has a high docking score of -7.9 Kcal/mol. SPD has a strong affinity with the thymidine kinase (PDB id: 1OF1) producing hydrogen bond and non-polar interaction at the target point of amino acid residue. Conclusion: Molecular docking analysis provides new insight into the structure-based design of SPD compounds with better antiviral activity against HSV-1.

show abstract

In vitro virucidal activity of a styrylpyrone derivative against herpes simplex virus strain KOS-1

Cited by 6 publications

References 0 publications

In vitro virucidal activity of Kyllinga nemoralis aqueous extract against herpes simplex virus

In vitro virucidal activity of Kyllinga nemoralis aqueous extract against herpes simplex virus

Antiviral Activity of an Extract from Leaves of the Tropical Plant Cynometra cauliflora

Styrylpyrone Derivative from Goniothalamus sp.: A Powerful Drug for Fighting Against Herpes Simplex Virus Type 1

Contact Info

Product

Resources

About