In vitro viability of lymphoid cells from lines of mice genetically selected for high or low responsiveness to phytohemagglutinin

An improved method for preparing G-banded chromosomes from mouse peripheral blood

“…It has been reported that PHA and Con A are T-cell specific mitogens (Stobo et al, 1972), whereas LPS is a specific B-cell stimulator (Andersson and Hayry, 1972). It also appears that B-cell response may be under separate genetic control from T-cell response, since selection for high T-cell response to PHA in mice does not select coordinately for high B-cell response (Stiffel et al, 1983). Whatever the precise mechanism, metaphase yield in cultures from low-responding or older mice can be improved by adding LPS.…”