In Vitro Versus in Situ Ruminal Biohydrogenation of Unsaturated Fatty Acids from a Raw or Extruded Mixture of Ground Canola Seed/Canola Meal

Enjalbert, Francis; Eynard, Philippe; Nicot, Marie-Claude; Troegeler‐Meynadier, Annabelle; Bayourthe, Corine; Moncoulon, R.

doi:10.3168/jds.s0022-0302(03)73613-3

Cited by 37 publications

(62 citation statements)

References 27 publications

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“…Previously, the in vitro biohydrogenation rate of 18:1c-9 was reportedly similar between raw and extruded canola, being half that of PUFA (Enjalbert et al, 2003). When feeding ruminants processed canola, the lower biohydrogenation rate and higher intestinal absorption of 18:1c-9 has resulted in an appreciable transfer to milk (Tymchuk et al, 1998) and adipose tissue (Huard et al, 1998).…”

Section: Total Lipidsmentioning

confidence: 99%

Influence of oilseed supplement ranging in n-6/n-3 ratio on fatty acid composition and Δ5-, Δ6-desaturase protein expression in steer muscles

Turner

Mitchell

Duynisveld

et al. 2012

Animal

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This study investigated effects of roasted or extruded oilseed supplementation ranging in n-6/n-3 ratios from 0.3 to 5.0 on the fatty acid composition and expression of delta-5 desaturase (D5d) and D6-desaturase (D6d) protein in commercial steer cheek (m. masseter) and diaphragm (pars costalis diaphragmatis) muscles. In general, the n-6/n-3 ratio of the diet had a subsequent effect on the muscle n-6/n-3 ratio (P , 0.05), with muscle 18:2n-6 and 18:3n-3 content relating to proportion of dietary soya bean and linseed (P , 0.01). Compared with canola, pure linseed and soya bean diets reduced 14:1c-9 and 16:1c-9 (P , 0.05) but increased 18:1t-11 and c-9,t-11 conjugated linoleic acid (CLA) content (P , 0.01). Oilseed processing had a minor influence but extruded oilseeds increase 18:1t-11 and c-9,t-11 CLA compared with roasted (P , 0.05). Polar lipid 18:3n-3 and n-3 long-chain polyunsaturated fatty acid (LC, >20 carbons PUFA) derivative content increased in relation to dietary linseed supplementation in the diaphragm (P , 0.01), whereas only 18:3n-3 was increased in the cheek (P , 0.01). Protein expression did not differ between diets; however, in each muscle the D5d protein expression had a stronger association with the desaturase products rather than the precursors. The relationship between D5d protein expression and the muscle LC n-6/n-3 ratio was negative in both muscles (P , 0.05). The relationship between D6d protein expression and the LC n-6/n-3 ratio was positive in the cheek (P , 0.001) and negative in the diaphragm (P , 0.05). In conclusion, diet n-6/n-3 ratio affected muscle 18:2n-6 and 18:3n-3 deposition, whereas the D5d and D6d protein expression had some influence on the polar lipid LC-PUFA profile. Results reaffirm that processed oilseeds can be used to increase the proportion of fatty acids potentially beneficial for human health, by influencing the formation of LC-PUFA and reducing the n-6/n-3 ratio.

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Section: Total Lipidsmentioning

confidence: 99%

Influence of oilseed supplement ranging in n-6/n-3 ratio on fatty acid composition and Δ5-, Δ6-desaturase protein expression in steer muscles

Turner

Mitchell

Duynisveld

et al. 2012

Animal

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“…for t L and P0P 0 for t 5L (Enjalbert et al 2003). a, b Values in the same row with different letters differ (P B0.05).…”

mentioning

confidence: 99%

“…The in situ methods have the advantage to incubate substrates in a more physiological milieu than in vitro procedures (Enjalbert et al 2003) and no information is available on the use of the in situ method to study the effects of ionophores on ruminal biohydrogenation of unsaturated FA. Therefore, the present study was conducted to compare the effects of lasalocid or monensin on biohydrogenation of C 18:2 from sunflower seed and C 18:3 from flaxseed using the in situ procedure.…”

mentioning

confidence: 99%

“…The model proposed by Enjalbert et al (2003) relies on the decrease in unsaturated FA proportions in the bag residue relative to proportions in the non-incubated 0 h substrate. This model corrects for physical losses of unsaturated FA from the polyester bags that would result in an overestimation of biohydrogenation.…”

mentioning

confidence: 99%

“…Ruminal biohydrogenation parameters were calculated using the following model suggested by Enjalbert et al (2003):…”

mentioning

confidence: 99%

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Effects of lasalocid or monensin on in situ biohydrogenation of flaxseed and sunflower seed unsaturated fatty acids

Martineau¹,

Petit²,

Benchaar³

et al. 2008

Can. J. Anim. Sci.

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R. 2008. Effects of lasalocid or monensin on in situ biohydrogenation of flaxseed and sunflower seed unsaturated fatty acids. Can. J. Anim. Sci. 88: 335Á339. The objective of this study was to compare the effects of two ionophores, lasalocid and monensin (24 mg kg (1 dry matter), on in situ biohydrogenation of C 18:2 and C 18:3 from whole sunflower seed and flaxseed, respectively. There were no treatment effects on biohydrogenation of C 18:2 . Ionophore supplementation did not affect the lag time, but monensin decreased the rate of biohydrogenation of C 18:3 compared with control and lasalocid.Key words: Ionophore, monensin, lasalocid, biohydrogenation (in situ), sunflower seed, flaxseed Martineau, R., Petit, H. V., Benchaar, C., Lapierre, H., Ouellet, D. R., Pellerin, D. et Berthiaume, R. 2008. Effets du lasalocid ou du monensin sur la biohydroge´nation ruminale in situ des acides gras insature´s du lin et du tournesol. Can. J. Anim. Sci. 88: 335Á339. L'objectif de cette e´tude e´tait de comparer les effets du lasalocid ou monensin sur la biohydroge´nation in situ du C 18:2 et du C 18:3 provenant respectivement du tournesol ou du lin entier. Il n'y a pas eu d'effets significatifs sur la biohydroge´nation du C 18:2 . La comple´mentation en ionophore n'a pas affecte´le temps de re´ponse mais le monensin a diminue´le taux de biohydroge´nation du C 18:3 comparativement au te´moin et au lasalocid.

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Assessing rumen biohydrogenation and its manipulation in vivo, in vitro and in situ

Fievez

Vlaeminck

Jenkins

et al. 2007

Euro J Lipid Sci & Tech

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Assessing rumen biohydrogenation and its manipulation in vivo, in vitro and in situReference techniques to study rumen biohydrogenation (BH) rely on the comparison of intake and duodenal or (ab)omasal flows of polyunsaturated fatty acids (PUFA), whereas the net BH of PUFA to their saturated end-products gives a quantitative measure of accumulating BH intermediates. The current review paper aims at evaluating alternative in vivo, in vitro and in sacco techniques to simulate reference in vivo results of unprotected PUFA sources, as well as strategies for overcoming or manipulating BH. In vivo rumen sampling approaches show potential but require further investigation, whereas in sacco results are inappropriate. In vitro 24-h batch incubations and continuous cultures approach in vivo BH of unprotected C 18 PUFA sources and are useful to assess the pH value at which dissociation of calcium salts of fatty acids occurs, but overestimate the degree of rumen inertness of formaldehyde-treated oil(seeds) and marine products. Batch or continuous cultures provide an accurate estimate (0.6) of the proportion of hydrogenated C 18 PUFA that are converted into their saturated end-product, except for incubations with high levels of fermentable substrate (.1.0 g/100 mL) in combination with high amounts of C 18 PUFA (.0.5 mg/mL) or in incubations with EPA and DHA.

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In Vitro Versus in Situ Ruminal Biohydrogenation of Unsaturated Fatty Acids from a Raw or Extruded Mixture of Ground Canola Seed/Canola Meal

Cited by 37 publications

References 27 publications

Influence of oilseed supplement ranging in n-6/n-3 ratio on fatty acid composition and Δ5-, Δ6-desaturase protein expression in steer muscles

Influence of oilseed supplement ranging in n-6/n-3 ratio on fatty acid composition and Δ5-, Δ6-desaturase protein expression in steer muscles

Effects of lasalocid or monensin on in situ biohydrogenation of flaxseed and sunflower seed unsaturated fatty acids

Assessing rumen biohydrogenation and its manipulation in vivo, in vitro and in situ

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