In vitro vasorelaxation mechanisms of bioactive compounds extracted from Hibiscus sabdariffa on rat thoracic aorta

Abstract

BackgroundIn this study, we suggested characterizing the vasodilator effects and the phytochemical characteristics of a plant with food usage also used in traditional treatment of arterial high blood pressure in Senegal.MethodsVascular effects of crude extract of dried and powdered calyces of Hibiscus sabdariffa were evaluated on isolated thoracic aorta of male Wistar rats on organ chambers. The crude extract was also enriched by liquid-liquid extraction. The various cyclohexane, dichloromethane, ethyl acetate, butanol extracts obtained as well as the residual marc were subjected to Sephadex LH-20 column chromatography. The different methanolic eluate fractions were then analyzed by Thin Layer (TLC) and High Performance Liquid Chromatography (HPLC) and their vascular effects also evaluated.ResultsThe H. Sabdariffa crude extract induced mainly endothelium-dependent relaxant effects. The endothelium-dependent relaxations result from NOS activation and those who not dependent to endothelium from activation of smooth muscle potassium channels. The phytochemical analysis revealed the presence of phenolic acids in the ethyl acetate extract and anthocyans in the butanolic extract. The biological efficiency of the various studied extracts, in term of vasorelaxant capacity, showed that: Butanol extract > Crude extract > Residual marc > Ethyl acetate extract. These results suggest that the strong activity of the butanolic extract is essentially due to the presence of anthocyans found in its fractions 43-67.ConclusionThese results demonstrate the vasodilator potential of hibiscus sabdariffa and contribute to his valuation as therapeutic alternative.

Detection of potential AcrAB-TolC multidrug efflux pump inhibitor in calyces extract of Hibiscus sabdariffa

“…Separation of H. sabdariffa extract on TLC showed similar profile to that been reported by Sarr et al, which was interpreted as following; blue zone: Phenolic acids, yellow-orange: Flavonols, and yellowgreen: Flavones [32]. TLC-bioassay plate overlaid with bacterial medium agar suspension only one inhibition zone, corresponding to phenolic acids, was formed which is related to the antimicrobial compound.…”

“…The ×10 concentrated extract was subjected to separation by TLC, 50 µl were loaded on 20 cm width TLC plates (Macherey-Nagel, Germany), separation was conducted by ethyl acetate/acetic acid/formic acid/water mixture (100:11:11:26) [32]. After drying, two plates were overlaid, separately; one with E. coli TG1-LB suspension (500 µl of OD 600 ~1.0 in 50 ml LB -7.5% agar) supplemented with a sub-lethal concentration of CV 0.78 µg/ml, the other plate was overlaid with E. coli TG1-LB.…”

Roselle Calyx Ethanol Extract Stimulates Oral Mucosal Wound Healing

“…The aim of wound care, which must occur in a physiologic environment conducive to tissue repair and regeneration, is to promote healing in the shortest time possible, to prevent secondary infections, and to minimize pain, discomfort, and scarring [3,4]. Wound healing is a complex process that involves three main phases: (1) The activation of different types of inflammatory cells or responses, (2) the formulation of granulation tissue as a result of cell proliferation during healing, and (3) the remodeling of tissues [1,5]. The healing process is influenced by several factors such as infection, nutrition, drugs, and hormones; the type, depth, and sites of wounds and certain disease conditions may also impact healing [4].…”

“…Localized topical regimens are considered standard treatment for mild cases of oral ulcers [4,5,9]. Many indigenous tribes around the world have long used annual herbaceous plants for their wound healing properties, although these have not received the attention of orthodox medical practitioners as potential sources of healing agents that could prove useful in the treatment of wounds or oral mucosal ulcers [1,3,4].…”

“…A number of medicinal plants, such as Jatropha cucas, Morinda citrifolia, Cassia alata, and Hibiscus sabdariffa Linn. (roselle), among others, have been employed in folk medicine for wound care [5]. Many parts of the roselle plant, for instance, are of value, including the leaves, yellow or reddish calyxes, seeds, and fibers.…”