In vitro UV-Visible and FTIR Spectroscopy Study of Low Power He-Ne Laser Irradiation on Human Blood

Abstract

Laser irradiation has biostimulating effect in various cell types low power laser radiation is used clinically for skin and vascular disorders.
 Aims: To investigate the effect of (He-Ne) laser (λ= 632nm, power=1mW) on human whole blood, after irradiated to different times from 10 min to 50 min.
 Study Design: Human Whole Blood Irradiated to (He-Ne) laser(λ= 632nm, power=1mW). 
 Place and Duration of Study: Soba Hospital, Khartoum- Sudan, Institute of Laser, Sudan University of science and technology (SUST), February 2018.
 Methodology: Blood samples were taken from healthy volunteers; blood sample irradiated to (He-Ne) laser and control compared; FTIR and UV-Vis spectrophotometer were used to study laser radiation effect
 Results: FTIR spectra and UV-vis absorption spectra of blood samples are compared before and after irradiated to He-Ne laser shows increases absorption for 10, and 40 min but it decreases as the exposure time at 30,20 and 50 minutes. This fluctuation of light absorption is known as a biphasic response.
 FTIR spectra of non exposed blood showed the peaks due to O-H (free group), C=O (amide I group), N=O (nitro group), and C-H (aromatic group). N-H (Amino acid (amide II). For all exposure time He-Ne laser (λ= 632nm, power=1mW) irradiation, showed significant changes. Increased in transmittance at different exposure time for all groups (C=O, O-H, N=O, C-O & C-H, N-H) and indicates significant decreasing in their concentration.
 Conclusion: The laser radiation causes changes in the structure and conformational changes in the polypeptide and absorption of blood samples decreased due to increasing ligand electronegativity.

Influence of High Power Nd: YAG Laser on Hardness and Surface Properties of Zirconium Silicate

“…There have been numerous research studies the effect of laser matter irradiation; for example, laser milk pasteurization (Marouf and Sara, 2018;Amna and Marouf, 2018), solar cell surface modification (Marouf et al, 2014, Islam et al, 2020, bee honey irradiation (Al Humira and Marouf, 2017) and production of highly value materials from agricultural waste (Gawbah et al, 2017;Gawbah et al, 2018). There have been numerous research studies the interaction of lowlevel lasers with biological materials such as blood; for examples; studding of He-Ne laser effect on human whole blood (Haimid et al, 2019a), investigating the effect of He-Ne laser on human whole blood (Haimid et al, 2019b), and it also used to induce emission in human teeth to distinguish www.universepg.com l UniversePG between dental caries and sound teeth (Marouf and Khairallah, 2019).…”