In-Vitro Uptake of Zinc by Boar Spermatozoa

Westmoreland, Nelson; First, N. L.; Hoekstra, W. G.

doi:10.1530/jrf.0.0130223

Cited by 20 publications

(8 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This may be partly due to the absorption of zinc by the spermatozoa suggested by the work of Westmoreland et al (1967) and confirmed by results of our own to be reported shortly.…”

Section: Other Secretionssupporting

confidence: 86%

“…Apart from the values for whole semen reported by Westmoreland, First & Hoekstra (1967), there is no information in the literature on the zinc content of the seminal fluids of the boar. As part of an investigation of the occurrence and functions of zinc in the reproductive system of this animal, we have determined the zinc content of forty-six samples of seminal plasma and seventeen samples of vesicular secretion.…”

Section: Introductionmentioning

confidence: 97%

See 1 more Smart Citation

The Concentrations of Zinc in Boar Seminal Plasma and Vesicular Secretion in Relation to Those of Nitrogenous Substances, Citrate, Galactose and Fructose

Boursnell¹,

Baronos²,

Briggs³

et al. 1972

Reproduction

View full text Add to dashboard Cite

Forty-five samples of seminal plasma obtained from eight boars and seventeen samples of vesicular secretion collected from individual boars had mean zinc contents of 22\m=.\7and 137\m=.\4 \g=m\g/ml, respectively. It was concluded that most of the zinc present in the seminal plasma of the boar is derived from the vesicular secretion.The mean concentrations of the organic constituents studied were: Seminal plasma: total nitrogen 4\m=.\42mg/ml (non-dialysable 88\m=.\7%), citrate 1 \m=.\28 mg/ml. Vesicular secretion: total nitrogen 15\m=.\1 mg/ml (non-dialysable 93\m=.\9%), citrate 6\m=.\07mg/ml, fructose 0\m=.\557mg/ ml, galactose 0\m=.\092 mg/ml. In both seminal plasma and vesicular secretion, the concentration of zinc was positively correlated with that of total and non-dialysable nitrogen and citrate. The correlation with nitrogenous substances was particularly strong in the seminal plasma of individual animals and there was evidence from the partial correlation coefficients that these associations were independent of the zinc-citrate relationship. However, fructose and galactose which do not have an appreciable affinity for zinc under physiological conditions also showed significant positive correlations with zinc (in vesicular secretion) and most of the organic constituents were interrelated in this way, indicating that in such data the influence of chemical combination may be masked by that of other factors which regulate the composition of these fluids.

show abstract

“…This may be partly due to the absorption of zinc by the spermatozoa suggested by the work of Westmoreland et al (1967) and confirmed by results of our own to be reported shortly.…”

Section: Other Secretionssupporting

confidence: 86%

Section: Introductionmentioning

confidence: 97%

The Concentrations of Zinc in Boar Seminal Plasma and Vesicular Secretion in Relation to Those of Nitrogenous Substances, Citrate, Galactose and Fructose

Boursnell¹,

Baronos²,

Briggs³

et al. 1972

Reproduction

View full text Add to dashboard Cite

show abstract

“…With respect to Zn, it has been shown in human that low seminal plasma Zn is associated with low motility, low sperm vitality, and infertility [Chia et al 2000]. In boars it has been shown that, when sperm cells are damaged, Zn accumulates in the sperm cells with a consequent reduction of Zn in SP [Westmoreland et al 1967].…”

Section: Discussionmentioning

confidence: 99%

Boar seminal plasma components and their relation with semen quality

Rodriguez

Rijsselaere

Beek

et al. 2012

Systems Biology in Reproductive Medicine

View full text Add to dashboard Cite

Select boar seminal plasma (SP) components and their relation to semen quality were investigated. Thirty nine boars from three artificial insemination (AI) centers were divided into group A (GA: > 80% normal sperm and >70% motility) and group B (GB: < 80% normal sperm and < 70% motility). Each ejaculate was collected and semen volume, concentration, sperm motility (computer aided semen analysis; CASA), morphology, and vitality (both eosin nigrosin staining) were investigated. The SP was separated and analyzed for aspartate-amino-transferase (AST), γ-glutamyl-transferase (GGT), alkaline phosphatase (ALP) activity, and the concentrations of sodium (Na), potassium (K), chloride (Cl), calcium (Ca), phosphate (PO 4 3-), magnesium (Mg), selenium (Se) and zinc (Zn) were assessed. Repeated measures (2 months interval) were conducted in eight boars of GA from one AI center. The activity of GGT (r = -0.482) and ALP (r = -0.459) was moderately associated ( p < 0.05) with ejaculate volume and strongly associated with concentration (r = 0.580 and r = 0.618, respectively; p = 0.000). Moderate associations ( p < 0.05) were found between ALP (r = 0.439), GGT (r = 0.387), Na (r = -0.428), K (r = 0.354), and Se (r = 0.354) with progressive motility. The SP concentration of Na (r = -0.401), Cl (r = -0.521), and K (r = 0.350) was associated ( p < 0.05) with normal morphology. Only Mg was associated ( p < 0.05) with membrane damage (r = -0.335). The concentration of Na, Cl, and Zn (1681.0 vs. 1701.0 µg/dL) was different between groups ( p < 0.05). Repeated measures showed significant differences in time but only for Na, Mg, and Zn ( p < 0.05). In conclusion, several biochemical components of SP were related to semen quality. The analysis of biochemical parameters could provide extra information about reproductive health of AI boars.

show abstract

“…Removal of zinc from the spermatozoa results in increased oxygen consumption after addition of succinate (Eliasson, Johnsen & Lindholmer, 1971;Huacuja et al, 1973) and increased formation of lipid peroxides (Johnsen & Eliasson, 1978a). The uptake or release of zinc by spermatozoa varies with species (Birnbaum, Hall & Lee, 1961;Lindholmer & Eliasson, 1974 (Westmoreland, First & Hoekstra, 1967).…”

Section: Introductionmentioning

confidence: 99%