In Vitro Uptake of Estradiol by the Anterior Hypothalamus and Hypophysis of the Rat

Kato, Junzo; Inaba, Machiko; Kobayashi, Takuya; Kobayashi, Takashi

doi:10.1530/acta.0.061s193

Cited by 3 publications

(2 citation statements)

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“…The presence of an oestradiol-binding species in the supernatant fraction of the hypothalamus (and possibly also in the cerebellumn) is indicated, but its function is unknown at present. These data, together with previous studies (Eisenfeld & Axelrod, 1966;Kato & Villee, 1967a,b;McGuire & Lisk, 1968;Kato, Inaba, Kobayashi, & Kobayashi, 1969) substantiate the role of the hypothalamus as a target tissue for oestrogen and also demonstrate the nucleus as a primary site for intracellular binding. The specificity ofuptake and retention ofoestradiol into the nucleus may, however, reside with cytoplasmic factors rather than with any inherent nuclear mechanism for hormone concentration.…”

Section: Discussionsupporting

confidence: 79%

Uptake of oestradiol by the rabbit hypothalamus. Specificity of binding by nuclei in vitro

Chader

Villee

1970

Biochemical Journal

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The binding of [6,7-(3)H]oestradiol-17beta to subcellular fractions of the hypothalamus and the cerebellum of the rabbit was studied in vitro. Uptake of steroid was higher in hypothalamic nuclei than in cerebellar nuclei. Lower binding was observed in other fractions of both tissues. After dialysis of the fractions, hypothalamic nuclei retained a high percentage of oestradiol whereas cerebellar nuclei lost most of the bound steroid. Supernatant fractions of both tissues retained a significant proportion of label after dialysis and after gel filtration on Sephadex G-200. No specific binding was observed in these fractions when subjected to sucrose-density-gradient centrifugation. Purification of nuclei followed by incubation with labelled oestradiol in the absence of the supernatant fraction resulted in loss of binding of steroid by hypothalamic nuclei. Incubation of the purified hypothalamic nuclei with supernatant fraction maintained the binding specificity of hormone retention.

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Section: Discussionsupporting

confidence: 79%

Uptake of oestradiol by the rabbit hypothalamus. Specificity of binding by nuclei in vitro

Chader

Villee

1970

Biochemical Journal

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“…The concentration of uterine estradiol binding sites increases fourfold between day 1 and 10 and then remains relatively constant through day 22 [4]. A concentration gradient between pituitary and plasma is observed after day 10 in rats [11]. In a study starting at day 5, a concentration of labeled estradiol in median eminence and hypothalamus calculated as ratio to cerebral cortex is not observed until day 20-30 [20].…”

Section: Umbilical Vessel Levels Of Estrone and Estradiol: Normalsmentioning

confidence: 95%

Unconjugated Estrogens in the Perinatal Period

Angsusingha

Stinson

et al. 1973

Pediatr Res

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ExtractHigh levels of unconjugated estrone ((Ei) 3-hydroxyestra-l ,3,5(10)-triene-17-one) and estradiol ((E 2 ) estra-1,3,5(10)-triene-3,17/3-diol) were found at term in maternal venous, umbilical vein, and umbilical artery plasma. For estrone the respective values in nanograms per milliliter ±SD were 12.8 ± 5.9, 25.1 ± 6.5, and 13.2 ± 7.7. For estradiol the respective values were 17.3 ± 9.2, 8.1 ± 4.0, and 5.1 ± 3.2. For estrone, levels for umbilical vein were higher than those in the paired maternal and umbilical artery; estradiol was higher in maternal vein than in the paired umbilical vessels. Absence of the fetal adrenals was associated with low levels of estrone and estradiol for maternal and umbilical vessels, whereas, in anencephaly, only the maternal levels were markedly diminished. An initially rapid, and then a slower decline in both estrogens was found in normal infants during the first 72 hr of life. Speculation

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Effect of Oestradiol-3,17ß Dipropionate on Spermatogenesis and Lysosomal Enzymes in the Rat Tests

Elkington¹,

Blackshaw²

1971

Aust. Jnl. Of Bio. Sci.

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Daily intramuscular injections (20 f-Lgjday) of oestradiol-3, 17,8 dipropionate in adult male rats depressed body growth and reduced the weight of the testes and seminal vesicles between 4 and 8 days of treatment.The proportion of soluble acid phosphatase (E.C. 3.1.3.2), acid proteinase (E.C. 3.4.4.23), and ,8-glucuronidase (E.C. 3.2.1.31), increased during oestradiol treatment. There was also an increase in the total amount of acid proteinase and ,8.glucuronidase but no significant change in total acid phosphatase activity with oestrogen treatment. These changes in hydrolase activity coincided with a decrease in the yield of late primary spermatocytes from early primary spermatocytes, and long spermatidsfromroundspermatids. Histochemical evidence supported the biochemical and histochemical findings, with increasing tubular staining of acid phosphatase and lipid in the testis during treatment.It is concluded that lysosomal enzymes are important in testicular degeneration and probably in normal spermatogenesis.

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In Vitro Uptake of Estradiol by the Anterior Hypothalamus and Hypophysis of the Rat

Cited by 3 publications

References 0 publications

Uptake of oestradiol by the rabbit hypothalamus. Specificity of binding by nuclei in vitro

Uptake of oestradiol by the rabbit hypothalamus. Specificity of binding by nuclei in vitro

Unconjugated Estrogens in the Perinatal Period

Effect of Oestradiol-3,17ß Dipropionate on Spermatogenesis and Lysosomal Enzymes in the Rat Tests

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