In vitro trypanocidal activity of the Egyptian plant Schinopsis lorentizii against trypomastigote and amastigote forms of Trypanosoma cruzi

Rashed, Khaled; Ferreira, Daniele da Silva; Esperandim, Viviane Rodrigues; Marçal, Maria Gabriela; Sequeira, Breno Mumic; Flauzino, Lúzio Gabriel Bocalon; Cunha, Wilson Roberto

doi:10.7324/japs.2016.60610

Cited by 7 publications

(5 citation statements)

References 16 publications

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“…The microplate was incubated at 37 °C for 24 hours. Afterwards, the activity was quantitatively verified by counting the trypomastigote forms in agreement with the technique previously described in the literature (Rashed et al 2016 (Esperandim et al 2013).…”

Section: In Vitro Evaluation Of Trypanocidal and Cytotoxic Activitiesmentioning

confidence: 86%

In vitro antiparasitic activity and chemical composition of the essential oil from Protium ovatum leaves (Burceraceae)

Estevam

Deus

Silva

et al. 2017

An. Acad. Bras. Ciênc.

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Leishmaniasis and trypanosomiasis are globally widespread parasitic diseases which have been responsible for high mortality rates. Since drugs available for their treatment are highly hepatotoxic, nephrotoxic and cardiotoxic, adherence to therapy has been affected. Thus, the search for new, more effective and safer drugs for the treatment of these diseases is necessary. Natural products have stood out as an alternative to searching for new bioactive molecules with therapeutic potential. In this study, the chemical composition and antiparasitic activity of the essential oil from Protium ovatum leaves against trypomastigote forms of Trypanosoma cruzi and the promastigote forms of Leishmania amazonensis were evaluated. The essential oil was promising against trypomastigote forms of T. cruzi (IC 50 = 28.55 μg.mL -1 ) and L.amazonensis promastigotes (IC 50 = 2.28 μg.mL -1 ). Eighteen chemical constituents were identified by GasChromatography coupled to Mass Spectrometry (GC-MS) in the essential oil, whose major constituents were spathulenol (17.6 %), caryophyllene oxide (16.4 %), β-caryophyllene (14.0 %) and myrcene (8.4 %).In addition, the essential oil from P. ovatum leaves had moderate cytotoxicity against LLCMK 2 adherent epithelial cell at the concentration range under analysis (CC 50 = 150.9 μg.mL -1 ). It should be highlighted that this is the first report of the chemical composition and anti-Trypanosoma cruzi and anti-Leishmania amazonensis activities of the essential oil from Protium ovatum leaves.

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Section: In Vitro Evaluation Of Trypanocidal and Cytotoxic Activitiesmentioning

confidence: 86%

In vitro antiparasitic activity and chemical composition of the essential oil from Protium ovatum leaves (Burceraceae)

Estevam

Deus

Silva

et al. 2017

An. Acad. Bras. Ciênc.

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“…The trypomastigote forms were maintained in RPMI medium and the parasites were transferred to fresh medium every 48 h to furnish free parasite forms. The assay conducted after 24 h was based on the methodology of Rashed et al (2016). Approximately 1 x 10 6 trypomastigotes was added to each well in a 96-well microtiter plate.…”

Section: In Vitro Trypanocidal and Cytotoxic Activitiesmentioning

confidence: 99%

“…All experiments were performed in triplicate. The percentage of cell viability was determined by the following formula: % cell viability = 1 -[(Y-N)/(N-P)] x 100, where Y = absorbance of wells containing cells and essential oil at different concentrations; N = negative control; and P = positive control (Rashed et al, 2016). (Adams, 2007); %RA: relative area (peak area relative to the total peak area in the GC-FID chromatogram).…”

Section: In Vitro Trypanocidal and Cytotoxic Activitiesmentioning

confidence: 99%

Chemical composition and biological activities of essential oil from flowers of Psidium guajava (Myrtaceae)

et al. 2021

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Xylella fastidiosa is a plant-pathogenic bacterium that lives inside host xylem vessels, where it forms biofilm which is believed to be responsible for disrupting the passage of water and nutrients. Pectobacterium carotovorum is a Gram-negative plant-specific bacterium that causes not only soft rot in various plant hosts, but also blackleg in potato by plant cell wall degradation. Chagas disease, which is caused by Trypanosoma cruzi, has been commonly treated with nifurtimox and benzonidazole, two drugs that cause several side effects. As a result, the use of natural products for treating bacterial and neglected diseases has increased in recent years and plants have become a promising alternative to developing new medicines. Therefore, this study aimed to determine, for the first time, the chemical composition of essential oil from Psidium guajava flowers (PG-EO) and to evaluate its in vitro anti-Xylella fastidiosa, anti-Pectobacterium carotovorum, anti-Trypanosoma cruzi and cytotoxic activities. PG-EO was obtained by hydrodistillation in a Clevenger apparatus while its chemical composition was determined by gas chromatography-flame ionization detection (GC-FID) and gas chromatography-mass spectrometry (GC-MS). Major compounds identified in PG-EO were α-cadinol (37.8%), β-caryophyllene (12.2%), nerolidol (9.1%), α-selinene (8.8%), β-selinene (7.4%) and caryophyllene oxide (7.2%). Results showed that the PG-EO had strong trypanocidal activity against the trypomastigote forms of Trypanosoma cruzi (IC50 = 14.6 μg/mL), promising antibacterial activity against X. fastidiosa (MIC = 12.5 μg/mL) and P. carotovorum (MIC = 62.5 μg/mL), and moderate cytotoxicity against LLCMK2 adherent epithelial cells in the concentration range (CC50 = 250.5 μg/mL). In short, the PG-EO can be considered a new source of bioactive compounds for the development of pesticides and trypanocide drugs.

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“…Microplate was incubated at 37.0 ºC, 5.0 % of CO2 for 24 hours. The anti-T. cruzi activity of microalgae extracts were evaluated by direct quantification of the parasites in a Neubauer chamber (Rashed et al 2016), in which parasite lyses percentage was determined by comparison of the control group and treatment. The efficacy of C. vulgaris and T. obliquus on T. cruzi was determined according Osorio et al (2007), classified as highly active, active, moderately active and inactive.…”

Section: Antiparasitic Assaymentioning

confidence: 99%

Green microalgae as a potential source of trypanocide compounds

Júnior

Silva

Oliveira³

et al. 2023

Natural Product Research

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Due to the limitations of Chagas disease therapy, microalgae can be promising in the search of new trypanocidal compounds, since these organisms produce bioactive compounds with large pharmaceutical applications, including antiparasitic effects. In this work, trypanocidal activity of aqueous extract of Tetradesmus obliquus and, for the first time, aqueous extract of Chlorella vulgaris, were evaluated against trypomastigote forms of Trypanosoma cruzi. In addition, cytotoxic activity employing Vero cells was evaluated. Our results showed that C. vulgaris and T. obliquus present trypanocidal activity (IC50 = 32.9 µg ml -1 and 36.4 µg ml -1 , respectively), however, C. vulgaris did not present cytotoxic effects in Vero cells (CC50 > 600 µg ml -1 ) and displayed a higher selectivity against trypomastigotes forms of T. cruzi (SI > 18). Thus, microalgae extracts, particularly the aqueous extract of C. vulgaris, are promising potential candidates for the development of natural antichagasic drugs.

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In vitro trypanocidal activity of the Egyptian plant Schinopsis lorentizii against trypomastigote and amastigote forms of Trypanosoma cruzi

Cited by 7 publications

References 16 publications

In vitro antiparasitic activity and chemical composition of the essential oil from Protium ovatum leaves (Burceraceae)

In vitro antiparasitic activity and chemical composition of the essential oil from Protium ovatum leaves (Burceraceae)

Chemical composition and biological activities of essential oil from flowers of Psidium guajava (Myrtaceae)

Green microalgae as a potential source of trypanocide compounds

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